Synchrotron-based infrared microspectroscopy unveils the biomolecular response of healthy and tumour cell lines to neon minibeam radiation therapy

Radioresistant tumours remain complex to manage with current radiotherapy (RT) techniques. Heavy ion beams were proposed for their treatment given their advantageous radiobiological properties. However, previous studies with patients resulted in serious adverse effects in the surrounding healthy tis...

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Autores: González-Vegas, Roberto|||0009-0000-4806-3092, Seksek, Olivier|||0000-0002-3053-3022, Bertho, Annaïg|||0009-0002-4526-0861, Bergs, J., Hirayama, R., Inaniwa, Taku, Matsufuji, N., Shimokawa, Takashi|||0000-0001-6549-9277, Prezado, Yolanda|||0000-0001-5957-2327, Yousef, Ibraheem|||0000-0001-7818-8611, Martínez-Rovira, Immaculada|||0000-0002-2918-489X
Tipo de recurso: artículo
Fecha de publicación:2024
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:310126
Acceso en línea:https://ddd.uab.cat/record/310126
https://dx.doi.org/urn:doi:10.1039/d4an01038h
Access Level:acceso abierto
Palabra clave:Damage
Ftir
Ions
Spectroscopy
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spelling Synchrotron-based infrared microspectroscopy unveils the biomolecular response of healthy and tumour cell lines to neon minibeam radiation therapyGonzález-Vegas, Roberto|||0009-0000-4806-3092Seksek, Olivier|||0000-0002-3053-3022Bertho, Annaïg|||0009-0002-4526-0861Bergs, J.Hirayama, R.Inaniwa, TakuMatsufuji, N.Shimokawa, Takashi|||0000-0001-6549-9277Prezado, Yolanda|||0000-0001-5957-2327Yousef, Ibraheem|||0000-0001-7818-8611Martínez-Rovira, Immaculada|||0000-0002-2918-489XDamageFtirIonsSpectroscopyRadioresistant tumours remain complex to manage with current radiotherapy (RT) techniques. Heavy ion beams were proposed for their treatment given their advantageous radiobiological properties. However, previous studies with patients resulted in serious adverse effects in the surrounding healthy tissues. Heavy ion RT could therefore benefit from the tissue-sparing effects of minibeam radiation therapy (MBRT). To investigate the potential of this combination, here we assessed the biochemical response to neon MBRT (NeMBRT) through synchrotron-based Fourier transform infrared microspectroscopy (SR-FTIRM). Healthy (BJ) and tumour (B16-F10) cell lines were subjected to seamless (broad beam) neon RT (NeBB) and NeMBRT at HIMAC. SR-FTIRM measurements were conducted at the MIRAS beamline of ALBA Synchrotron. Principal component analysis (PCA) permitted to assess the biochemical effects after the irradiations and 24 hours post-irradiation for the different RT modalities and doses. For the healthy cells, NeMBRT resulted in the most dissimilar spectral signatures from non-irradiated cells early after irradiations, mainly due to protein conformational modifications. Nevertheless, most of the damage appeared to recover one day post-RT; conversely, protein- and nucleic acid-related IR bands were strongly affected by NeBB 24 hours after treatment, suggesting superior oxidative damage and nucleic acid degradation. Tumour cells appeared to be less sensitive to NeBB than to NeMBRT shortly after RT. Still, after one day, both NeBB and the high-dose NeMBRT regions yielded important spectral modifications, suggestive of cell death processes, protein oxidation or oxidative stress. Lipid-associated spectral changes, especially due to the NeBB and NeMBRT peak groups for the tumour cell line, were consistent with reactive oxygen species attacks. 22024-01-0120242024-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/310126https://dx.doi.org/urn:doi:10.1039/d4an01038hreponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengEuropean Commission https://doi.org/10.13039/501100000780 817908Ministerio de Ciencia, Innovación y Universidades https://doi.org/10.13039/100014440 PRE2021-097298Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2020-114079RA-I00Ministerio de Ciencia, Innovación y Universidades https://doi.org/10.13039/100014440 RYC2018-024043-IAgència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2021/SGR-00607open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:3101262026-06-06T12:50:31Z
dc.title.none.fl_str_mv Synchrotron-based infrared microspectroscopy unveils the biomolecular response of healthy and tumour cell lines to neon minibeam radiation therapy
title Synchrotron-based infrared microspectroscopy unveils the biomolecular response of healthy and tumour cell lines to neon minibeam radiation therapy
spellingShingle Synchrotron-based infrared microspectroscopy unveils the biomolecular response of healthy and tumour cell lines to neon minibeam radiation therapy
González-Vegas, Roberto|||0009-0000-4806-3092
Damage
Ftir
Ions
Spectroscopy
title_short Synchrotron-based infrared microspectroscopy unveils the biomolecular response of healthy and tumour cell lines to neon minibeam radiation therapy
title_full Synchrotron-based infrared microspectroscopy unveils the biomolecular response of healthy and tumour cell lines to neon minibeam radiation therapy
title_fullStr Synchrotron-based infrared microspectroscopy unveils the biomolecular response of healthy and tumour cell lines to neon minibeam radiation therapy
title_full_unstemmed Synchrotron-based infrared microspectroscopy unveils the biomolecular response of healthy and tumour cell lines to neon minibeam radiation therapy
title_sort Synchrotron-based infrared microspectroscopy unveils the biomolecular response of healthy and tumour cell lines to neon minibeam radiation therapy
dc.creator.none.fl_str_mv González-Vegas, Roberto|||0009-0000-4806-3092
Seksek, Olivier|||0000-0002-3053-3022
Bertho, Annaïg|||0009-0002-4526-0861
Bergs, J.
Hirayama, R.
Inaniwa, Taku
Matsufuji, N.
Shimokawa, Takashi|||0000-0001-6549-9277
Prezado, Yolanda|||0000-0001-5957-2327
Yousef, Ibraheem|||0000-0001-7818-8611
Martínez-Rovira, Immaculada|||0000-0002-2918-489X
author González-Vegas, Roberto|||0009-0000-4806-3092
author_facet González-Vegas, Roberto|||0009-0000-4806-3092
Seksek, Olivier|||0000-0002-3053-3022
Bertho, Annaïg|||0009-0002-4526-0861
Bergs, J.
Hirayama, R.
Inaniwa, Taku
Matsufuji, N.
Shimokawa, Takashi|||0000-0001-6549-9277
Prezado, Yolanda|||0000-0001-5957-2327
Yousef, Ibraheem|||0000-0001-7818-8611
Martínez-Rovira, Immaculada|||0000-0002-2918-489X
author_role author
author2 Seksek, Olivier|||0000-0002-3053-3022
Bertho, Annaïg|||0009-0002-4526-0861
Bergs, J.
Hirayama, R.
Inaniwa, Taku
Matsufuji, N.
Shimokawa, Takashi|||0000-0001-6549-9277
Prezado, Yolanda|||0000-0001-5957-2327
Yousef, Ibraheem|||0000-0001-7818-8611
Martínez-Rovira, Immaculada|||0000-0002-2918-489X
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Damage
Ftir
Ions
Spectroscopy
topic Damage
Ftir
Ions
Spectroscopy
description Radioresistant tumours remain complex to manage with current radiotherapy (RT) techniques. Heavy ion beams were proposed for their treatment given their advantageous radiobiological properties. However, previous studies with patients resulted in serious adverse effects in the surrounding healthy tissues. Heavy ion RT could therefore benefit from the tissue-sparing effects of minibeam radiation therapy (MBRT). To investigate the potential of this combination, here we assessed the biochemical response to neon MBRT (NeMBRT) through synchrotron-based Fourier transform infrared microspectroscopy (SR-FTIRM). Healthy (BJ) and tumour (B16-F10) cell lines were subjected to seamless (broad beam) neon RT (NeBB) and NeMBRT at HIMAC. SR-FTIRM measurements were conducted at the MIRAS beamline of ALBA Synchrotron. Principal component analysis (PCA) permitted to assess the biochemical effects after the irradiations and 24 hours post-irradiation for the different RT modalities and doses. For the healthy cells, NeMBRT resulted in the most dissimilar spectral signatures from non-irradiated cells early after irradiations, mainly due to protein conformational modifications. Nevertheless, most of the damage appeared to recover one day post-RT; conversely, protein- and nucleic acid-related IR bands were strongly affected by NeBB 24 hours after treatment, suggesting superior oxidative damage and nucleic acid degradation. Tumour cells appeared to be less sensitive to NeBB than to NeMBRT shortly after RT. Still, after one day, both NeBB and the high-dose NeMBRT regions yielded important spectral modifications, suggestive of cell death processes, protein oxidation or oxidative stress. Lipid-associated spectral changes, especially due to the NeBB and NeMBRT peak groups for the tumour cell line, were consistent with reactive oxygen species attacks.
publishDate 2024
dc.date.none.fl_str_mv 2
2024-01-01
2024
2024-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/310126
https://dx.doi.org/urn:doi:10.1039/d4an01038h
url https://ddd.uab.cat/record/310126
https://dx.doi.org/urn:doi:10.1039/d4an01038h
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv European Commission https://doi.org/10.13039/501100000780 817908
Ministerio de Ciencia, Innovación y Universidades https://doi.org/10.13039/100014440 PRE2021-097298
Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2020-114079RA-I00
Ministerio de Ciencia, Innovación y Universidades https://doi.org/10.13039/100014440 RYC2018-024043-I
Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2021/SGR-00607
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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