Efficacy of Environmental Sampling Devices for Listeria monocytogenes Detection in a Ready-to-Eat Production Facility

[EN] Listeria monocytogenes is considered a highly persistent risk for public health in food production facilities. Food business operators manufacturing ready-to-eat foods (RTE) are required to sample processing areas for L. monocytogenes as part of their environmental monitoring plans. The aim of...

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Autores: Tomás Fornés, David, Fornés-Pérez, Alba|||0000-0002-9058-5959, Barat Baviera, José Manuel|||0000-0001-8487-7114, Moreno Trigos, Mª Yolanda|||0000-0003-3688-5157, Fuentes López, Ana|||0000-0002-4144-2396
Tipo de recurso: artículo
Fecha de publicación:2026
País:España
Institución:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:dnet:riunet______::e0746bf67c0f37e447d9ff23269c8f35
Acceso en línea:https://riunet.upv.es/handle/10251/235973
Access Level:acceso abierto
Palabra clave:Listeria spp.
Listeria monocytogenes
Surface sampling
Environmental monitoring
Biofilm formation
Microbial contamination
Test method
Microbial genomics
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spelling Efficacy of Environmental Sampling Devices for Listeria monocytogenes Detection in a Ready-to-Eat Production FacilityTomás Fornés, DavidFornés-Pérez, Alba|||0000-0002-9058-5959Barat Baviera, José Manuel|||0000-0001-8487-7114Moreno Trigos, Mª Yolanda|||0000-0003-3688-5157Fuentes López, Ana|||0000-0002-4144-2396Listeria spp.Listeria monocytogenesSurface samplingEnvironmental monitoringBiofilm formationMicrobial contaminationTest methodMicrobial genomics[EN] Listeria monocytogenes is considered a highly persistent risk for public health in food production facilities. Food business operators manufacturing ready-to-eat foods (RTE) are required to sample processing areas for L. monocytogenes as part of their environmental monitoring plans. The aim of the study was to identify suitable sampling devices, demonstrating the crucial role of the sampling technique in the method performance for L. monocytogenes monitoring. Detection of Listeria spp. and L. monocytogenes from surfaces in an RTE food production facility was evaluated by using two different sampling methods (swabs and sponges). When using swabs, 46 sampling points were negative for both targets. However, when sampling same points with sponges, 30% samples (14 out of 46) were positive for Listeria spp. with 8 samples (17%) positive for L. monocytogenes. During subsequent in vitro experiments, L. monocytogenes strains spiked onto three different surfaces (stainless steel, Teflon and epoxy) showed recoveries of between 76% and 93% when using sponges, while in swabs, recoveries where always below 50%. All L. monocytogenes strains isolated belonged to the major clonal complexes (CC) circulating in Europe in food industry (e.g., CC121 and CC9) and none of them are considered among the hypervirulent strains. Genomic analysis, including new tools for source tracking (Gene Up Typer, bioMérieux) showed differences between strains isolated from different risk hygienic zones.MDPI AGDepartamento de Tecnología de AlimentosInstituto Universitario de Ingeniería del Agua y del Medio AmbienteInstituto Universitario de Ingeniería de Alimentos (FoodUPV)Escuela Técnica Superior de Ingeniería Agronómica y del Medio NaturalGrupo de Investigación e Innovación AlimentariaRepositorio Institucional de la Universitat Politècnica de València Riunet20262026-04-10journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://riunet.upv.es/handle/10251/235973reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Reconocimiento (by)http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:dnet:riunet______::e0746bf67c0f37e447d9ff23269c8f352026-06-13T07:49:27Z
dc.title.none.fl_str_mv Efficacy of Environmental Sampling Devices for Listeria monocytogenes Detection in a Ready-to-Eat Production Facility
title Efficacy of Environmental Sampling Devices for Listeria monocytogenes Detection in a Ready-to-Eat Production Facility
spellingShingle Efficacy of Environmental Sampling Devices for Listeria monocytogenes Detection in a Ready-to-Eat Production Facility
Tomás Fornés, David
Listeria spp.
Listeria monocytogenes
Surface sampling
Environmental monitoring
Biofilm formation
Microbial contamination
Test method
Microbial genomics
title_short Efficacy of Environmental Sampling Devices for Listeria monocytogenes Detection in a Ready-to-Eat Production Facility
title_full Efficacy of Environmental Sampling Devices for Listeria monocytogenes Detection in a Ready-to-Eat Production Facility
title_fullStr Efficacy of Environmental Sampling Devices for Listeria monocytogenes Detection in a Ready-to-Eat Production Facility
title_full_unstemmed Efficacy of Environmental Sampling Devices for Listeria monocytogenes Detection in a Ready-to-Eat Production Facility
title_sort Efficacy of Environmental Sampling Devices for Listeria monocytogenes Detection in a Ready-to-Eat Production Facility
dc.creator.none.fl_str_mv Tomás Fornés, David
Fornés-Pérez, Alba|||0000-0002-9058-5959
Barat Baviera, José Manuel|||0000-0001-8487-7114
Moreno Trigos, Mª Yolanda|||0000-0003-3688-5157
Fuentes López, Ana|||0000-0002-4144-2396
author Tomás Fornés, David
author_facet Tomás Fornés, David
Fornés-Pérez, Alba|||0000-0002-9058-5959
Barat Baviera, José Manuel|||0000-0001-8487-7114
Moreno Trigos, Mª Yolanda|||0000-0003-3688-5157
Fuentes López, Ana|||0000-0002-4144-2396
author_role author
author2 Fornés-Pérez, Alba|||0000-0002-9058-5959
Barat Baviera, José Manuel|||0000-0001-8487-7114
Moreno Trigos, Mª Yolanda|||0000-0003-3688-5157
Fuentes López, Ana|||0000-0002-4144-2396
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Departamento de Tecnología de Alimentos
Instituto Universitario de Ingeniería del Agua y del Medio Ambiente
Instituto Universitario de Ingeniería de Alimentos (FoodUPV)
Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural
Grupo de Investigación e Innovación Alimentaria
Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv Listeria spp.
Listeria monocytogenes
Surface sampling
Environmental monitoring
Biofilm formation
Microbial contamination
Test method
Microbial genomics
topic Listeria spp.
Listeria monocytogenes
Surface sampling
Environmental monitoring
Biofilm formation
Microbial contamination
Test method
Microbial genomics
description [EN] Listeria monocytogenes is considered a highly persistent risk for public health in food production facilities. Food business operators manufacturing ready-to-eat foods (RTE) are required to sample processing areas for L. monocytogenes as part of their environmental monitoring plans. The aim of the study was to identify suitable sampling devices, demonstrating the crucial role of the sampling technique in the method performance for L. monocytogenes monitoring. Detection of Listeria spp. and L. monocytogenes from surfaces in an RTE food production facility was evaluated by using two different sampling methods (swabs and sponges). When using swabs, 46 sampling points were negative for both targets. However, when sampling same points with sponges, 30% samples (14 out of 46) were positive for Listeria spp. with 8 samples (17%) positive for L. monocytogenes. During subsequent in vitro experiments, L. monocytogenes strains spiked onto three different surfaces (stainless steel, Teflon and epoxy) showed recoveries of between 76% and 93% when using sponges, while in swabs, recoveries where always below 50%. All L. monocytogenes strains isolated belonged to the major clonal complexes (CC) circulating in Europe in food industry (e.g., CC121 and CC9) and none of them are considered among the hypervirulent strains. Genomic analysis, including new tools for source tracking (Gene Up Typer, bioMérieux) showed differences between strains isolated from different risk hygienic zones.
publishDate 2026
dc.date.none.fl_str_mv 2026
2026-04-10
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://riunet.upv.es/handle/10251/235973
url https://riunet.upv.es/handle/10251/235973
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento (by)
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento (by)
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI AG
publisher.none.fl_str_mv MDPI AG
dc.source.none.fl_str_mv reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname:Universitat Politècnica de València (UPV)
instname_str Universitat Politècnica de València (UPV)
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
repository.name.fl_str_mv
repository.mail.fl_str_mv
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