Dendritic platinum nanoparticles shielded by Pt-S PEGylation as intracellular reactors for bioorthogonal uncaging chemistry
Beyond their classical role as cytotoxics, Platinum (Pt) coordination complexes recently joined the selected group of transition metals capable of performing bioorthogonal reactions in living environments. To minimize their reactivity towards nucleophiles, which limit their catalytic performance, we...
| Autores: | , , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2025 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/389639 |
| Acceso en línea: | http://hdl.handle.net/10261/389639 |
| Access Level: | acceso abierto |
| Palabra clave: | Anticancer drugs Platinum Bioorthogonal Heterogeneous Catalysis |
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España |
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| dc.title.none.fl_str_mv |
Dendritic platinum nanoparticles shielded by Pt-S PEGylation as intracellular reactors for bioorthogonal uncaging chemistry |
| title |
Dendritic platinum nanoparticles shielded by Pt-S PEGylation as intracellular reactors for bioorthogonal uncaging chemistry |
| spellingShingle |
Dendritic platinum nanoparticles shielded by Pt-S PEGylation as intracellular reactors for bioorthogonal uncaging chemistry García-Peiro, José I. Anticancer drugs Platinum Bioorthogonal Heterogeneous Catalysis |
| title_short |
Dendritic platinum nanoparticles shielded by Pt-S PEGylation as intracellular reactors for bioorthogonal uncaging chemistry |
| title_full |
Dendritic platinum nanoparticles shielded by Pt-S PEGylation as intracellular reactors for bioorthogonal uncaging chemistry |
| title_fullStr |
Dendritic platinum nanoparticles shielded by Pt-S PEGylation as intracellular reactors for bioorthogonal uncaging chemistry |
| title_full_unstemmed |
Dendritic platinum nanoparticles shielded by Pt-S PEGylation as intracellular reactors for bioorthogonal uncaging chemistry |
| title_sort |
Dendritic platinum nanoparticles shielded by Pt-S PEGylation as intracellular reactors for bioorthogonal uncaging chemistry |
| dc.creator.none.fl_str_mv |
García-Peiro, José I. Ortega-Liébana, M. Carmen Adam, Catherine Lorente-Macías, Álvaro Travnickova, Jana Patton, E. Elizabeth Guerrero-López, Paula García-Aznar, José Manuel Hueso, José L. Santamaría, Jesús Unciti-Broceta, Asier |
| author |
García-Peiro, José I. |
| author_facet |
García-Peiro, José I. Ortega-Liébana, M. Carmen Adam, Catherine Lorente-Macías, Álvaro Travnickova, Jana Patton, E. Elizabeth Guerrero-López, Paula García-Aznar, José Manuel Hueso, José L. Santamaría, Jesús Unciti-Broceta, Asier |
| author_role |
author |
| author2 |
Ortega-Liébana, M. Carmen Adam, Catherine Lorente-Macías, Álvaro Travnickova, Jana Patton, E. Elizabeth Guerrero-López, Paula García-Aznar, José Manuel Hueso, José L. Santamaría, Jesús Unciti-Broceta, Asier |
| author2_role |
author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
European Commission European Research Council Engineering and Physical Sciences Research Council (UK) Melanoma Research Alliance (US) Cancer Research UK Junta de Andalucía Gobierno de Aragón Zebra Foundation Agencia Estatal de Investigación (España) Ministerio de Ciencia, Innovación y Universidades (España) Instituto de Salud Carlos III Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72] |
| dc.subject.none.fl_str_mv |
Anticancer drugs Platinum Bioorthogonal Heterogeneous Catalysis |
| topic |
Anticancer drugs Platinum Bioorthogonal Heterogeneous Catalysis |
| description |
Beyond their classical role as cytotoxics, Platinum (Pt) coordination complexes recently joined the selected group of transition metals capable of performing bioorthogonal reactions in living environments. To minimize their reactivity towards nucleophiles, which limit their catalytic performance, we investigated the use of Pt(0) with different forms, sizes and surface functionalization. We report herein the development of PEGylated Pt nanodendrites with the capacity to activate prodyes and prodrugs in cell culture and in vivo. Their dendritic morphology together with their surface shielding through Pt-S-bonded PEGylation synergistically contributed to create catalytic nanoreactors compatible with the highly-crowded and reductive environment of the cell cytoplasm, thereby facilitating in situ bioorthogonal drug uncaging in cancer cells in 2D and 3D culture, including in microfluidic systems, and xenografted in zebrafish. |
| publishDate |
2025 |
| dc.date.none.fl_str_mv |
2025 2025 2025 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article http://purl.org/coar/resource_type/c_6501 Publisher's version info:eu-repo/semantics/publishedVersion |
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article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/10261/389639 |
| url |
http://hdl.handle.net/10261/389639 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
#PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/EC/H2020/841990 info:eu-repo/grantAgreement/EC/H2020/742684 info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/CEX2023-001286-S The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.1002/anie.202424037 https://doi.org/10.1002/anie.202424037 Sí |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
| dc.publisher.none.fl_str_mv |
Wiley-VCH |
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Wiley-VCH |
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reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC instname:Consejo Superior de Investigaciones Científicas (CSIC) |
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Consejo Superior de Investigaciones Científicas (CSIC) |
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DIGITAL.CSIC. Repositorio Institucional del CSIC |
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DIGITAL.CSIC. Repositorio Institucional del CSIC |
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1869425238870065152 |
| spelling |
Dendritic platinum nanoparticles shielded by Pt-S PEGylation as intracellular reactors for bioorthogonal uncaging chemistryGarcía-Peiro, José I.Ortega-Liébana, M. CarmenAdam, CatherineLorente-Macías, ÁlvaroTravnickova, JanaPatton, E. ElizabethGuerrero-López, PaulaGarcía-Aznar, José ManuelHueso, José L.Santamaría, JesúsUnciti-Broceta, AsierAnticancer drugsPlatinumBioorthogonalHeterogeneousCatalysisBeyond their classical role as cytotoxics, Platinum (Pt) coordination complexes recently joined the selected group of transition metals capable of performing bioorthogonal reactions in living environments. To minimize their reactivity towards nucleophiles, which limit their catalytic performance, we investigated the use of Pt(0) with different forms, sizes and surface functionalization. We report herein the development of PEGylated Pt nanodendrites with the capacity to activate prodyes and prodrugs in cell culture and in vivo. Their dendritic morphology together with their surface shielding through Pt-S-bonded PEGylation synergistically contributed to create catalytic nanoreactors compatible with the highly-crowded and reductive environment of the cell cytoplasm, thereby facilitating in situ bioorthogonal drug uncaging in cancer cells in 2D and 3D culture, including in microfluidic systems, and xenografted in zebrafish.We are grateful to the EC (H2020-MSCA-IF-2018-841990) and EPSRC (EP/S010289/1) for financial support. We also would like to acknowledge the European Research Council (ERC) through the Catalytic Dual-Function Devices Against Cancer (CADENCE-742684) project, RLDI/2015-021. EEP is funded by the MRC (MC_UU_00035/13), Melanoma Research Alliance and Rosetrees Trust (MRA Awards 687306, 917226). This work was supported by the Cancer Research UK Scotland Centre (CTRQQR-2021\100006). MCOL is grateful to the Institute of Genetics and Cancer (IGC) for financial support through the Hastie Career Advancement Fund 2020, and the Consejeria de Universidad Investigacion e Innovacion by ERDF Andalusian Program 2021–2027 (A-EXP-278-UGR23). We thank IGC Zebrafish facility for supporting zebrafish management and IGC Imaging Facility for their support with imaging experiments. P.G.-L. gratefully acknowledges the support of the Government of Aragon (Grant No. 2021–25). The synthesis of materials has been performed by the Platform of Production of Biomaterials and Nanoparticles of the NANBIOSIS ICTS, more specifically by the Nanoparticle Synthesis Unit of the CIBER in BioEngineering, Biomaterials & Nanomedicine (CIBER-BBN). The TEM studies were conducted at the Laboratorio de Microscopias Avanzadas, Instituto de Nanociencia y Materiales de Aragon, Universidad de Zaragoza, Spain. (SAI and ELECMI). Ayuda CEX2023-001286-S financiada por MICIU/AEI/10.13039/501100011033.With funding from the Spanish government through the "Severo Ochoa Centre of Excellence" accreditation (CEX2023-001286-S).Peer reviewedWiley-VCHEuropean CommissionEuropean Research CouncilEngineering and Physical Sciences Research Council (UK)Melanoma Research Alliance (US)Cancer Research UKJunta de AndalucíaGobierno de AragónZebra FoundationAgencia Estatal de Investigación (España)Ministerio de Ciencia, Innovación y Universidades (España)Instituto de Salud Carlos IIIConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252025info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/389639reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/EC/H2020/841990info:eu-repo/grantAgreement/EC/H2020/742684info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/CEX2023-001286-SThe underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.1002/anie.202424037https://doi.org/10.1002/anie.202424037Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3896392026-05-22T06:33:51Z |
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15,81155 |