Design and Synthesis of Hollow Particles Based on Bacterial Cellulose and Polyhydroxybutyrate for Microbial Entrapping Using Coaxial Electrospray Technology

The design of biocarriers presents an effective approach for preserving bioactive elements and enabling controlled release in specific environments. This study introduces a novel biocarrier structure composed of two biodegradable, non-toxic, yet inherently incompatible bacterial biopolymers: bacteri...

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Autores: Rivero-Buceta, Virginia, García-Fernández, Luis, Campano, Cristina, Hernández-Herreros, Natalia, Aguilar, María Rosa, Prieto, M. Auxiliadora
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2025
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/385968
Acceso en línea:http://hdl.handle.net/10261/385968
Access Level:acceso abierto
Palabra clave:Bacterial cellulose
Polyhydroxyalkanoates
Double-shelled hollow particles
Electrospray
Bacterial trapping
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spelling Design and Synthesis of Hollow Particles Based on Bacterial Cellulose and Polyhydroxybutyrate for Microbial Entrapping Using Coaxial Electrospray TechnologyRivero-Buceta, VirginiaGarcía-Fernández, LuisCampano, CristinaHernández-Herreros, NataliaAguilar, María RosaPrieto, M. AuxiliadoraBacterial cellulosePolyhydroxyalkanoatesDouble-shelled hollow particlesElectrosprayBacterial trappingThe design of biocarriers presents an effective approach for preserving bioactive elements and enabling controlled release in specific environments. This study introduces a novel biocarrier structure composed of two biodegradable, non-toxic, yet inherently incompatible bacterial biopolymers: bacterial cellulose (BC), a hydrophilic porous polymer known for its high water-holding capacity (up to 400 times its dry weight) and tensile strength, and polyhydroxybutyrate (PHB), a hydrophobic polymer characterized by its excellent barrier properties and UV stability. Using a coaxial electrospray technique, double-shelled hollow particles (DSHP) with a spherical architecture and an average diameter of 360 µm were produced. These particles consist of an outer PHB shell that serves as a protective barrier, and an inner BC-based layer designed to support microbial viability. To ensure structural integrity and enhance compatibility between the polymers, PHB chains were grafted onto BC, achieving a modification degree of 31%, prior to electrospraying. The resulting DSHP demonstrated an internal cavity capable of housing bacterial loads up to 108 CFU/mL, maintaining cell viability for at least 2 days and enabling controlled release profile. Additionally, the optimized electrospray conditions ensured high reproducibility and stability. This promising particle configuration offers potential applicability across various fields, from biomedicine to environmental applications.The authors received financial support from the European Union’s Horizon 2020 Research and Innovation Program under grant agreement no. 870294 (Mix-Up), the CSIC Interdisciplinary Thematic Platform (PTI+) Sustainable Plastics towards a Circular Economy (PTI-Susplast+), the Community of Madrid (P2018/NMT4389), and the grants BIOCIR (PID2020-112766RB-C21) and OPENVIRO (PID2023-146557OB-C21) funded by MCIN/AEI/ 10.13039/501100011033.Peer reviewedElsevierEuropean CommissionConsejo Superior de Investigaciones Científicas (España)Comunidad de MadridMinisterio de Ciencia e Innovación (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252025info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/385968reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/EC/H2020/870294info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-112766RB-C21info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2023-146557OB-C21https://doi.org/10.1016/j.carpta.2025.100791Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3859682026-05-22T06:33:51Z
dc.title.none.fl_str_mv Design and Synthesis of Hollow Particles Based on Bacterial Cellulose and Polyhydroxybutyrate for Microbial Entrapping Using Coaxial Electrospray Technology
title Design and Synthesis of Hollow Particles Based on Bacterial Cellulose and Polyhydroxybutyrate for Microbial Entrapping Using Coaxial Electrospray Technology
spellingShingle Design and Synthesis of Hollow Particles Based on Bacterial Cellulose and Polyhydroxybutyrate for Microbial Entrapping Using Coaxial Electrospray Technology
Rivero-Buceta, Virginia
Bacterial cellulose
Polyhydroxyalkanoates
Double-shelled hollow particles
Electrospray
Bacterial trapping
title_short Design and Synthesis of Hollow Particles Based on Bacterial Cellulose and Polyhydroxybutyrate for Microbial Entrapping Using Coaxial Electrospray Technology
title_full Design and Synthesis of Hollow Particles Based on Bacterial Cellulose and Polyhydroxybutyrate for Microbial Entrapping Using Coaxial Electrospray Technology
title_fullStr Design and Synthesis of Hollow Particles Based on Bacterial Cellulose and Polyhydroxybutyrate for Microbial Entrapping Using Coaxial Electrospray Technology
title_full_unstemmed Design and Synthesis of Hollow Particles Based on Bacterial Cellulose and Polyhydroxybutyrate for Microbial Entrapping Using Coaxial Electrospray Technology
title_sort Design and Synthesis of Hollow Particles Based on Bacterial Cellulose and Polyhydroxybutyrate for Microbial Entrapping Using Coaxial Electrospray Technology
dc.creator.none.fl_str_mv Rivero-Buceta, Virginia
García-Fernández, Luis
Campano, Cristina
Hernández-Herreros, Natalia
Aguilar, María Rosa
Prieto, M. Auxiliadora
author Rivero-Buceta, Virginia
author_facet Rivero-Buceta, Virginia
García-Fernández, Luis
Campano, Cristina
Hernández-Herreros, Natalia
Aguilar, María Rosa
Prieto, M. Auxiliadora
author_role author
author2 García-Fernández, Luis
Campano, Cristina
Hernández-Herreros, Natalia
Aguilar, María Rosa
Prieto, M. Auxiliadora
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv European Commission
Consejo Superior de Investigaciones Científicas (España)
Comunidad de Madrid
Ministerio de Ciencia e Innovación (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Bacterial cellulose
Polyhydroxyalkanoates
Double-shelled hollow particles
Electrospray
Bacterial trapping
topic Bacterial cellulose
Polyhydroxyalkanoates
Double-shelled hollow particles
Electrospray
Bacterial trapping
description The design of biocarriers presents an effective approach for preserving bioactive elements and enabling controlled release in specific environments. This study introduces a novel biocarrier structure composed of two biodegradable, non-toxic, yet inherently incompatible bacterial biopolymers: bacterial cellulose (BC), a hydrophilic porous polymer known for its high water-holding capacity (up to 400 times its dry weight) and tensile strength, and polyhydroxybutyrate (PHB), a hydrophobic polymer characterized by its excellent barrier properties and UV stability. Using a coaxial electrospray technique, double-shelled hollow particles (DSHP) with a spherical architecture and an average diameter of 360 µm were produced. These particles consist of an outer PHB shell that serves as a protective barrier, and an inner BC-based layer designed to support microbial viability. To ensure structural integrity and enhance compatibility between the polymers, PHB chains were grafted onto BC, achieving a modification degree of 31%, prior to electrospraying. The resulting DSHP demonstrated an internal cavity capable of housing bacterial loads up to 108 CFU/mL, maintaining cell viability for at least 2 days and enabling controlled release profile. Additionally, the optimized electrospray conditions ensured high reproducibility and stability. This promising particle configuration offers potential applicability across various fields, from biomedicine to environmental applications.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/385968
url http://hdl.handle.net/10261/385968
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/EC/H2020/870294
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-112766RB-C21
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2023-146557OB-C21
https://doi.org/10.1016/j.carpta.2025.100791
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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