Analysis of autophagic vesicles in mitotic cells

The detection of autophagic vesicles in interphase cells is well characterized with markers such as LC3, SQSTM1 (also known as p62) and LAMP2, which are commonly used in immunofluorescence and biochemistry assays to evaluate the status of autophagy in adherent cells. During mitosis, cells undergo im...

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Detalles Bibliográficos
Autores: Almacellas i Canals, Eugènia, García Cajide, Marta, Tauler Girona, Albert, Mauvezin, Caroline
Tipo de recurso: capítulo de libro
Fecha de publicación:2022
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/209208
Acceso en línea:https://hdl.handle.net/2445/209208
Access Level:acceso abierto
Palabra clave:Lisosomes
Autofàgia
Lysosomes
Autophagy
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spelling Analysis of autophagic vesicles in mitotic cellsAlmacellas i Canals, EugèniaGarcía Cajide, MartaTauler Girona, AlbertMauvezin, CarolineLisosomesAutofàgiaLysosomesAutophagyThe detection of autophagic vesicles in interphase cells is well characterized with markers such as LC3, SQSTM1 (also known as p62) and LAMP2, which are commonly used in immunofluorescence and biochemistry assays to evaluate the status of autophagy in adherent cells. During mitosis, cells undergo important morphological changes which alter the position of the central plane, therefore the imaging on dividing cells has to be specifically designed. Here, we describe a method to label and image autophagic vesicles in mitotic cells to systematically analyze their number, morphology and distribution.20242022info:eu-repo/semantics/bookPart11 p.application/pdfapplication/pdfhttps://hdl.handle.net/2445/209208Llibres / Capítols de llibre (Bioquímica i Biomedicina Molecular)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésVersió postprint del capítol del llibre publicat a: https://doi.org/10.1007/978-1-0716-2071-7_9Autophagy and Cancer. Methods in Molecular Biology, vol 2445, Norberg, H., Norberg, E. (eds), ISBN: 978-1-0716-2071-7, p. 127-137https://doi.org/10.1007/978-1-0716-2071-7_9(c) Almacellas i Canals et al., 2022info:eu-repo/semantics/openAccessoai:recercat.cat:2445/2092082026-05-29T05:05:01Z
dc.title.none.fl_str_mv Analysis of autophagic vesicles in mitotic cells
title Analysis of autophagic vesicles in mitotic cells
spellingShingle Analysis of autophagic vesicles in mitotic cells
Almacellas i Canals, Eugènia
Lisosomes
Autofàgia
Lysosomes
Autophagy
title_short Analysis of autophagic vesicles in mitotic cells
title_full Analysis of autophagic vesicles in mitotic cells
title_fullStr Analysis of autophagic vesicles in mitotic cells
title_full_unstemmed Analysis of autophagic vesicles in mitotic cells
title_sort Analysis of autophagic vesicles in mitotic cells
dc.creator.none.fl_str_mv Almacellas i Canals, Eugènia
García Cajide, Marta
Tauler Girona, Albert
Mauvezin, Caroline
author Almacellas i Canals, Eugènia
author_facet Almacellas i Canals, Eugènia
García Cajide, Marta
Tauler Girona, Albert
Mauvezin, Caroline
author_role author
author2 García Cajide, Marta
Tauler Girona, Albert
Mauvezin, Caroline
author2_role author
author
author
dc.subject.none.fl_str_mv Lisosomes
Autofàgia
Lysosomes
Autophagy
topic Lisosomes
Autofàgia
Lysosomes
Autophagy
description The detection of autophagic vesicles in interphase cells is well characterized with markers such as LC3, SQSTM1 (also known as p62) and LAMP2, which are commonly used in immunofluorescence and biochemistry assays to evaluate the status of autophagy in adherent cells. During mitosis, cells undergo important morphological changes which alter the position of the central plane, therefore the imaging on dividing cells has to be specifically designed. Here, we describe a method to label and image autophagic vesicles in mitotic cells to systematically analyze their number, morphology and distribution.
publishDate 2022
dc.date.none.fl_str_mv 2022
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/bookPart
format bookPart
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/209208
url https://hdl.handle.net/2445/209208
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del capítol del llibre publicat a: https://doi.org/10.1007/978-1-0716-2071-7_9
Autophagy and Cancer. Methods in Molecular Biology, vol 2445, Norberg, H., Norberg, E. (eds), ISBN: 978-1-0716-2071-7, p. 127-137
https://doi.org/10.1007/978-1-0716-2071-7_9
dc.rights.none.fl_str_mv (c) Almacellas i Canals et al., 2022
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Almacellas i Canals et al., 2022
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 11 p.
application/pdf
application/pdf
dc.source.none.fl_str_mv Llibres / Capítols de llibre (Bioquímica i Biomedicina Molecular)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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