Molecular Detection and Identification of Chlamydiaceae in the Eyes of Wild and Domestic Ruminant Hosts from Northern Spain

Infections by Chlamydiae are associated with ocular disease in humans and animals. In this study, the presence and diversity of Chlamydia spp. was assessed in diseased and healthy eyes of domestic sheep and wild ruminants that share mountain habitats in northern Spain. The presence of Chlamydia spp....

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Autores: Dias-Alves, Andrea, Cabezón Ponsoda, Óscar, Borel, Nicole, López Olvera, Jorge R., Mentaberre García, Gregorio, Lavín González, Santiago, Fernández-Aguilar, Xavier
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Recursos:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10459.1/71054
Acesso em linha:https://doi.org/10.3390/pathogens10030383
http://hdl.handle.net/10459.1/71054
Access Level:acceso abierto
Palavra-chave:Chlamydia pecorum
Domestic sheep
Infectious keratoconjunctivitis
Ocular disease
Pyrenean chamois
Rupicapra pyrenaica
Wildlife-livestock interface
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spelling Molecular Detection and Identification of Chlamydiaceae in the Eyes of Wild and Domestic Ruminant Hosts from Northern SpainDias-Alves, AndreaCabezón Ponsoda, ÓscarBorel, NicoleLópez Olvera, Jorge R.Mentaberre García, GregorioLavín González, SantiagoFernández-Aguilar, XavierChlamydia pecorumDomestic sheepInfectious keratoconjunctivitisOcular diseasePyrenean chamoisRupicapra pyrenaicaWildlife-livestock interfaceInfections by Chlamydiae are associated with ocular disease in humans and animals. In this study, the presence and diversity of Chlamydia spp. was assessed in diseased and healthy eyes of domestic sheep and wild ruminants that share mountain habitats in northern Spain. The presence of Chlamydia spp. was tested by real-time PCR in 1786 conjunctival swabs collected from both eyes of 893 animals from mountain habitats in northern Spain, and chlamydial species were identified in the positive samples by ArrayTube microarray methods. Chlamydial DNA was detected in 0.6% (CI95% 0.2–1.3) of the Pyrenean chamois (Rupicapra pyrenaica) and 1.4% (CI95% <0.01–8.1) of the sheep (Ovis aries) sampled, with Chlamydia pecorum the only chlamydial species identified. No association of C. pecorum with ocular disease or co-infection with Mycoplasma conjunctivae was found. Further studies on the pathogenesis of infectious keratoconjunctivitis are needed to better understand the ecology of C. pecorum and its possible role as a ruminant pathogen at the wildlife–livestock interface.This study was funded by the research project CGL2009-11631 of the Spanish MICINN. X. Fernández Aguilar received a predoctoral grant (FI-DGR 2013) from the Generalitat de Catalunya.MDPI202120212021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://doi.org/10.3390/pathogens10030383http://hdl.handle.net/10459.1/71054http://hdl.handle.net/10459.1/71054reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)Inglésinfo:eu-repo/grantAgreement/MICINN//CGL2009-11631Reproducció del document publicat a: https://doi.org/10.3390/pathogens10030383Pathogens, 2021, vol. 10, núm. 3, article 383cc-by (c) Dias-Alves et al., 2021info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/oai:recercat.cat:10459.1/710542026-05-29T05:05:01Z
dc.title.none.fl_str_mv Molecular Detection and Identification of Chlamydiaceae in the Eyes of Wild and Domestic Ruminant Hosts from Northern Spain
title Molecular Detection and Identification of Chlamydiaceae in the Eyes of Wild and Domestic Ruminant Hosts from Northern Spain
spellingShingle Molecular Detection and Identification of Chlamydiaceae in the Eyes of Wild and Domestic Ruminant Hosts from Northern Spain
Dias-Alves, Andrea
Chlamydia pecorum
Domestic sheep
Infectious keratoconjunctivitis
Ocular disease
Pyrenean chamois
Rupicapra pyrenaica
Wildlife-livestock interface
title_short Molecular Detection and Identification of Chlamydiaceae in the Eyes of Wild and Domestic Ruminant Hosts from Northern Spain
title_full Molecular Detection and Identification of Chlamydiaceae in the Eyes of Wild and Domestic Ruminant Hosts from Northern Spain
title_fullStr Molecular Detection and Identification of Chlamydiaceae in the Eyes of Wild and Domestic Ruminant Hosts from Northern Spain
title_full_unstemmed Molecular Detection and Identification of Chlamydiaceae in the Eyes of Wild and Domestic Ruminant Hosts from Northern Spain
title_sort Molecular Detection and Identification of Chlamydiaceae in the Eyes of Wild and Domestic Ruminant Hosts from Northern Spain
dc.creator.none.fl_str_mv Dias-Alves, Andrea
Cabezón Ponsoda, Óscar
Borel, Nicole
López Olvera, Jorge R.
Mentaberre García, Gregorio
Lavín González, Santiago
Fernández-Aguilar, Xavier
author Dias-Alves, Andrea
author_facet Dias-Alves, Andrea
Cabezón Ponsoda, Óscar
Borel, Nicole
López Olvera, Jorge R.
Mentaberre García, Gregorio
Lavín González, Santiago
Fernández-Aguilar, Xavier
author_role author
author2 Cabezón Ponsoda, Óscar
Borel, Nicole
López Olvera, Jorge R.
Mentaberre García, Gregorio
Lavín González, Santiago
Fernández-Aguilar, Xavier
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Chlamydia pecorum
Domestic sheep
Infectious keratoconjunctivitis
Ocular disease
Pyrenean chamois
Rupicapra pyrenaica
Wildlife-livestock interface
topic Chlamydia pecorum
Domestic sheep
Infectious keratoconjunctivitis
Ocular disease
Pyrenean chamois
Rupicapra pyrenaica
Wildlife-livestock interface
description Infections by Chlamydiae are associated with ocular disease in humans and animals. In this study, the presence and diversity of Chlamydia spp. was assessed in diseased and healthy eyes of domestic sheep and wild ruminants that share mountain habitats in northern Spain. The presence of Chlamydia spp. was tested by real-time PCR in 1786 conjunctival swabs collected from both eyes of 893 animals from mountain habitats in northern Spain, and chlamydial species were identified in the positive samples by ArrayTube microarray methods. Chlamydial DNA was detected in 0.6% (CI95% 0.2–1.3) of the Pyrenean chamois (Rupicapra pyrenaica) and 1.4% (CI95% <0.01–8.1) of the sheep (Ovis aries) sampled, with Chlamydia pecorum the only chlamydial species identified. No association of C. pecorum with ocular disease or co-infection with Mycoplasma conjunctivae was found. Further studies on the pathogenesis of infectious keratoconjunctivitis are needed to better understand the ecology of C. pecorum and its possible role as a ruminant pathogen at the wildlife–livestock interface.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.3390/pathogens10030383
http://hdl.handle.net/10459.1/71054
http://hdl.handle.net/10459.1/71054
url https://doi.org/10.3390/pathogens10030383
http://hdl.handle.net/10459.1/71054
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/MICINN//CGL2009-11631
Reproducció del document publicat a: https://doi.org/10.3390/pathogens10030383
Pathogens, 2021, vol. 10, núm. 3, article 383
dc.rights.none.fl_str_mv cc-by (c) Dias-Alves et al., 2021
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
rights_invalid_str_mv cc-by (c) Dias-Alves et al., 2021
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812429

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