Piglet innate immune response to Streptococcus suis colonization is modulated by the virulence of the strain
Streptococcus suis is a zoonotic pathogen of swine involved in arthritis, polyserositis, and meningitis. Colonization of piglets by S. suis is very common and occurs early in life. The clinical outcome of infection is influenced by the virulence of the S. suis strains and the immunity of the animals...
| Autores: | , , , , , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2021 |
| País: | España |
| Institución: | Universitat Autònoma de Barcelona |
| Repositorio: | Dipòsit Digital de Documents de la UAB |
| Idioma: | inglés |
| OAI Identifier: | oai:ddd.uab.cat:266221 |
| Acceso en línea: | https://ddd.uab.cat/record/266221 https://dx.doi.org/urn:doi:10.1186/s13567-021-01013-w |
| Access Level: | acceso abierto |
| Palabra clave: | Streptococcus suis Colonization Innate immunity Pig immunity Gene expression Bacterial virulence |
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Piglet innate immune response to Streptococcus suis colonization is modulated by the virulence of the strainNeila-Ibáñez, Carlos María|||0000-0001-7233-2475Brogaard, Louise|||0000-0003-4571-6822Pailler-García, Lola|||0000-0003-1211-7057Martínez Martínez, Jorge|||0000-0001-5246-7276Segalés Coma, Joaquim|||0000-0002-1539-7261Segura, Mariela|||0000-0002-7489-4882Heegaard, Peter M. H.|||0000-0003-4506-4932Aragón, Virginia|||0000-0002-3470-6015Streptococcus suisColonizationInnate immunityPig immunityGene expressionBacterial virulenceStreptococcus suis is a zoonotic pathogen of swine involved in arthritis, polyserositis, and meningitis. Colonization of piglets by S. suis is very common and occurs early in life. The clinical outcome of infection is influenced by the virulence of the S. suis strains and the immunity of the animals. Here, the role of innate immunity was studied in cesarean-derived colostrum-deprived piglets inoculated intranasally with either virulent S. suis strain 10 (S10) or non-virulent S. suis strain T15. Colonization of the inoculated piglets was confirmed at the end of the study by PCR and immunohistochemistry. Fever (≥40.5 °C) was more prevalent in piglets inoculated with S10 compared to T15 at 4 h after inoculation. During the 3 days of monitoring, no other major clinical signs were detected. Accordingly, only small changes in transcription of genes associated with the antibacterial innate immune response were observed at systemic sites, with S10 inducing an earlier response than T15 in blood. Local inflammatory response to the inoculation, evaluated by transcriptional analysis of selected genes in nasal swabs, was more sustained in piglets inoculated with the virulent S10, as demonstrated by transcription of inflammation-related genes, such as IL1B, IL1A, and IRF7. In contrast, most of the gene expression changes in trachea, lungs, and associated lymph nodes were observed in response to the non-virulent T15 strain. Thus, S. suis colonization in the absence of systemic infection induces an innate immune response in piglets that appears to be related to the virulence potential of the colonizing strain. 22021-01-0120212021-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/266221https://dx.doi.org/urn:doi:10.1186/s13567-021-01013-wreponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengEuropean Commission https://doi.org/10.13039/501100000780 727966open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2662212026-06-06T12:50:31Z |
| dc.title.none.fl_str_mv |
Piglet innate immune response to Streptococcus suis colonization is modulated by the virulence of the strain |
| title |
Piglet innate immune response to Streptococcus suis colonization is modulated by the virulence of the strain |
| spellingShingle |
Piglet innate immune response to Streptococcus suis colonization is modulated by the virulence of the strain Neila-Ibáñez, Carlos María|||0000-0001-7233-2475 Streptococcus suis Colonization Innate immunity Pig immunity Gene expression Bacterial virulence |
| title_short |
Piglet innate immune response to Streptococcus suis colonization is modulated by the virulence of the strain |
| title_full |
Piglet innate immune response to Streptococcus suis colonization is modulated by the virulence of the strain |
| title_fullStr |
Piglet innate immune response to Streptococcus suis colonization is modulated by the virulence of the strain |
| title_full_unstemmed |
Piglet innate immune response to Streptococcus suis colonization is modulated by the virulence of the strain |
| title_sort |
Piglet innate immune response to Streptococcus suis colonization is modulated by the virulence of the strain |
| dc.creator.none.fl_str_mv |
Neila-Ibáñez, Carlos María|||0000-0001-7233-2475 Brogaard, Louise|||0000-0003-4571-6822 Pailler-García, Lola|||0000-0003-1211-7057 Martínez Martínez, Jorge|||0000-0001-5246-7276 Segalés Coma, Joaquim|||0000-0002-1539-7261 Segura, Mariela|||0000-0002-7489-4882 Heegaard, Peter M. H.|||0000-0003-4506-4932 Aragón, Virginia|||0000-0002-3470-6015 |
| author |
Neila-Ibáñez, Carlos María|||0000-0001-7233-2475 |
| author_facet |
Neila-Ibáñez, Carlos María|||0000-0001-7233-2475 Brogaard, Louise|||0000-0003-4571-6822 Pailler-García, Lola|||0000-0003-1211-7057 Martínez Martínez, Jorge|||0000-0001-5246-7276 Segalés Coma, Joaquim|||0000-0002-1539-7261 Segura, Mariela|||0000-0002-7489-4882 Heegaard, Peter M. H.|||0000-0003-4506-4932 Aragón, Virginia|||0000-0002-3470-6015 |
| author_role |
author |
| author2 |
Brogaard, Louise|||0000-0003-4571-6822 Pailler-García, Lola|||0000-0003-1211-7057 Martínez Martínez, Jorge|||0000-0001-5246-7276 Segalés Coma, Joaquim|||0000-0002-1539-7261 Segura, Mariela|||0000-0002-7489-4882 Heegaard, Peter M. H.|||0000-0003-4506-4932 Aragón, Virginia|||0000-0002-3470-6015 |
| author2_role |
author author author author author author author |
| dc.subject.none.fl_str_mv |
Streptococcus suis Colonization Innate immunity Pig immunity Gene expression Bacterial virulence |
| topic |
Streptococcus suis Colonization Innate immunity Pig immunity Gene expression Bacterial virulence |
| description |
Streptococcus suis is a zoonotic pathogen of swine involved in arthritis, polyserositis, and meningitis. Colonization of piglets by S. suis is very common and occurs early in life. The clinical outcome of infection is influenced by the virulence of the S. suis strains and the immunity of the animals. Here, the role of innate immunity was studied in cesarean-derived colostrum-deprived piglets inoculated intranasally with either virulent S. suis strain 10 (S10) or non-virulent S. suis strain T15. Colonization of the inoculated piglets was confirmed at the end of the study by PCR and immunohistochemistry. Fever (≥40.5 °C) was more prevalent in piglets inoculated with S10 compared to T15 at 4 h after inoculation. During the 3 days of monitoring, no other major clinical signs were detected. Accordingly, only small changes in transcription of genes associated with the antibacterial innate immune response were observed at systemic sites, with S10 inducing an earlier response than T15 in blood. Local inflammatory response to the inoculation, evaluated by transcriptional analysis of selected genes in nasal swabs, was more sustained in piglets inoculated with the virulent S10, as demonstrated by transcription of inflammation-related genes, such as IL1B, IL1A, and IRF7. In contrast, most of the gene expression changes in trachea, lungs, and associated lymph nodes were observed in response to the non-virulent T15 strain. Thus, S. suis colonization in the absence of systemic infection induces an innate immune response in piglets that appears to be related to the virulence potential of the colonizing strain. |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2 2021-01-01 2021 2021-01-01 |
| dc.type.none.fl_str_mv |
Article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://ddd.uab.cat/record/266221 https://dx.doi.org/urn:doi:10.1186/s13567-021-01013-w |
| url |
https://ddd.uab.cat/record/266221 https://dx.doi.org/urn:doi:10.1186/s13567-021-01013-w |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.relation.none.fl_str_mv |
European Commission https://doi.org/10.13039/501100000780 727966 |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/ |
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info:eu-repo/semantics/openAccess |
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open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/ |
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openAccess |
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application/pdf |
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reponame:Dipòsit Digital de Documents de la UAB instname:Universitat Autònoma de Barcelona |
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Universitat Autònoma de Barcelona |
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Dipòsit Digital de Documents de la UAB |
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