Nesfatin-1 in human and murine cardiomyocytes: synthesis, secretion, and mobilization of GLUT-4

Feijoo Bandin, Sandra; Rodríguez Penas, Diego; García Rua, Vanessa; Mosquera Leal, Ana; Otero Santiago, Manuel Francisco; Pereira Lopez, Eva Maria; Rubio Álvarez, José; Martínez Soto, María Isabel; Seoane Camino, Luisa Maria; Gualillo ., Oreste; Calaza Cabanas, Manuel; García-Caballero Parada, Tomas; Portolés, M.; Roselló-Lletí, E.; Diéguez González, Carlos; Rivera, M.; González Juanatey, José Ramón; Lago Paz, Francisca

Nesfatin-1 in human and murine cardiomyocytes: synthesis, secretion, and mobilization of GLUT-4

Nesfatin-1, a satiety-inducing peptide identified in hypothalamic regions that regulate energy balance, is an integral regulator of energy homeostasis and a putative glucose-dependent insulin coadjuvant. We investigated its production by human cardiomyocytes and its effects on glucose uptake, in the...

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Autores:	Feijoo Bandin, Sandra, Rodríguez Penas, Diego, García Rua, Vanessa, Mosquera Leal, Ana, Otero Santiago, Manuel Francisco, Pereira Lopez, Eva Maria, Rubio Álvarez, José, Martínez Soto, María Isabel, Seoane Camino, Luisa Maria, Gualillo ., Oreste, Calaza Cabanas, Manuel, García-Caballero Parada, Tomas, Portolés, M., Roselló-Lletí, E., Diéguez González, Carlos, Rivera, M., González Juanatey, José Ramón, Lago Paz, Francisca
Tipo de recurso:	artículo
Fecha de publicación:	2013
País:	España
Institución:	Servizo Galego de Saúde (SERGAS)
Repositorio:	RUNA. Repositorio da Consellería de Sanidade e Sergas
OAI Identifier:	oai:runa.sergas.gal:20.500.11940/1982
Acceso en línea:	http://hdl.handle.net/20.500.11940/1982
Access Level:	acceso abierto
Palabra clave:	Animal Feed Animals Calcium-Binding Proteins Cells, Cultured DNA-Binding Proteins Diet Dietary Fats/pharmacology Female Gene Expression Regulation Glucose Glucose Transporter Type 4 Humans Male Mice Myocytes, Cardiac Nerve Tissue Proteins Nucleobindins RNA, Messenger Rats Rats, Sprague-Dawley

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spelling	Nesfatin-1 in human and murine cardiomyocytes: synthesis, secretion, and mobilization of GLUT-4Feijoo Bandin, SandraRodríguez Penas, DiegoGarcía Rua, VanessaMosquera Leal, AnaOtero Santiago, Manuel FranciscoPereira Lopez, Eva MariaRubio Álvarez, JoséMartínez Soto, María IsabelSeoane Camino, Luisa MariaGualillo ., OresteCalaza Cabanas, ManuelGarcía-Caballero Parada, TomasPortolés, M.Roselló-Lletí, E.Diéguez González, CarlosRivera, M.González Juanatey, José RamónLago Paz, FranciscaAnimal FeedAnimalsCalcium-Binding ProteinsCells, CulturedDNA-Binding ProteinsDietDietary Fats/pharmacologyFemaleGene Expression RegulationGlucoseGlucose Transporter Type 4HumansMaleMiceMyocytes, CardiacNerve Tissue ProteinsNucleobindinsRNA, MessengerRatsRats, Sprague-DawleyNesfatin-1, a satiety-inducing peptide identified in hypothalamic regions that regulate energy balance, is an integral regulator of energy homeostasis and a putative glucose-dependent insulin coadjuvant. We investigated its production by human cardiomyocytes and its effects on glucose uptake, in the main cardiac glucose transporter GLUT-4 and in intracellular signaling. Quantitative RT-PCR, Western blots, confocal immunofluorescence microscopy, and ELISA of human and murine cardiomyocytes and/or cardiac tissue showed that cardiomyocytes can synthesize and secrete nesfatin-1. Confocal microscopy of cultured cardiomyocytes after GLUT-4 labeling showed that nesfatin-1 mobilizes this glucose transporter to cell peripherals. The rate of 2-deoxy-D-[(3)H]glucose incorporation demonstrated that nesfatin-1 induces glucose uptake by HL-1 cells and cultured cardiomyocytes. Nesfatin-1 induced dose- and time-dependent increases in the phosphorylation of ERK1/2, AKT, and AS160. In murine and human cardiac tissue, nesfatin-1 levels varied with diet and coronary health. In conclusion, human and murine cardiomyocytes can synthesize and secrete nesfatin-1, which is able to induce glucose uptake and the mobilization of the glucose transporter GLUT-4 in these cells. Nesfatin-1 cardiac levels are regulated by diet and coronary health.2013info:eu-repo/semantics/articlehttp://hdl.handle.net/20.500.11940/1982reponame:RUNA. Repositorio da Consellería de Sanidade e Sergasinstname:Servizo Galego de Saúde (SERGAS)Inglésinfo:eu-repo/semantics/openAccessoai:runa.sergas.gal:20.500.11940/19822026-06-12T08:40:47Z
dc.title.none.fl_str_mv	Nesfatin-1 in human and murine cardiomyocytes: synthesis, secretion, and mobilization of GLUT-4
title	Nesfatin-1 in human and murine cardiomyocytes: synthesis, secretion, and mobilization of GLUT-4
spellingShingle	Nesfatin-1 in human and murine cardiomyocytes: synthesis, secretion, and mobilization of GLUT-4 Feijoo Bandin, Sandra Animal Feed Animals Calcium-Binding Proteins Cells, Cultured DNA-Binding Proteins Diet Dietary Fats/pharmacology Female Gene Expression Regulation Glucose Glucose Transporter Type 4 Humans Male Mice Myocytes, Cardiac Nerve Tissue Proteins Nucleobindins RNA, Messenger Rats Rats, Sprague-Dawley
title_short	Nesfatin-1 in human and murine cardiomyocytes: synthesis, secretion, and mobilization of GLUT-4
title_full	Nesfatin-1 in human and murine cardiomyocytes: synthesis, secretion, and mobilization of GLUT-4
title_fullStr	Nesfatin-1 in human and murine cardiomyocytes: synthesis, secretion, and mobilization of GLUT-4
title_full_unstemmed	Nesfatin-1 in human and murine cardiomyocytes: synthesis, secretion, and mobilization of GLUT-4
title_sort	Nesfatin-1 in human and murine cardiomyocytes: synthesis, secretion, and mobilization of GLUT-4
dc.creator.none.fl_str_mv	Feijoo Bandin, Sandra Rodríguez Penas, Diego García Rua, Vanessa Mosquera Leal, Ana Otero Santiago, Manuel Francisco Pereira Lopez, Eva Maria Rubio Álvarez, José Martínez Soto, María Isabel Seoane Camino, Luisa Maria Gualillo ., Oreste Calaza Cabanas, Manuel García-Caballero Parada, Tomas Portolés, M. Roselló-Lletí, E. Diéguez González, Carlos Rivera, M. González Juanatey, José Ramón Lago Paz, Francisca
author	Feijoo Bandin, Sandra
author_facet	Feijoo Bandin, Sandra Rodríguez Penas, Diego García Rua, Vanessa Mosquera Leal, Ana Otero Santiago, Manuel Francisco Pereira Lopez, Eva Maria Rubio Álvarez, José Martínez Soto, María Isabel Seoane Camino, Luisa Maria Gualillo ., Oreste Calaza Cabanas, Manuel García-Caballero Parada, Tomas Portolés, M. Roselló-Lletí, E. Diéguez González, Carlos Rivera, M. González Juanatey, José Ramón Lago Paz, Francisca
author_role	author
author2	Rodríguez Penas, Diego García Rua, Vanessa Mosquera Leal, Ana Otero Santiago, Manuel Francisco Pereira Lopez, Eva Maria Rubio Álvarez, José Martínez Soto, María Isabel Seoane Camino, Luisa Maria Gualillo ., Oreste Calaza Cabanas, Manuel García-Caballero Parada, Tomas Portolés, M. Roselló-Lletí, E. Diéguez González, Carlos Rivera, M. González Juanatey, José Ramón Lago Paz, Francisca
author2_role	author author author author author author author author author author author author author author author author author
dc.subject.none.fl_str_mv	Animal Feed Animals Calcium-Binding Proteins Cells, Cultured DNA-Binding Proteins Diet Dietary Fats/pharmacology Female Gene Expression Regulation Glucose Glucose Transporter Type 4 Humans Male Mice Myocytes, Cardiac Nerve Tissue Proteins Nucleobindins RNA, Messenger Rats Rats, Sprague-Dawley
topic	Animal Feed Animals Calcium-Binding Proteins Cells, Cultured DNA-Binding Proteins Diet Dietary Fats/pharmacology Female Gene Expression Regulation Glucose Glucose Transporter Type 4 Humans Male Mice Myocytes, Cardiac Nerve Tissue Proteins Nucleobindins RNA, Messenger Rats Rats, Sprague-Dawley
description	Nesfatin-1, a satiety-inducing peptide identified in hypothalamic regions that regulate energy balance, is an integral regulator of energy homeostasis and a putative glucose-dependent insulin coadjuvant. We investigated its production by human cardiomyocytes and its effects on glucose uptake, in the main cardiac glucose transporter GLUT-4 and in intracellular signaling. Quantitative RT-PCR, Western blots, confocal immunofluorescence microscopy, and ELISA of human and murine cardiomyocytes and/or cardiac tissue showed that cardiomyocytes can synthesize and secrete nesfatin-1. Confocal microscopy of cultured cardiomyocytes after GLUT-4 labeling showed that nesfatin-1 mobilizes this glucose transporter to cell peripherals. The rate of 2-deoxy-D-[(3)H]glucose incorporation demonstrated that nesfatin-1 induces glucose uptake by HL-1 cells and cultured cardiomyocytes. Nesfatin-1 induced dose- and time-dependent increases in the phosphorylation of ERK1/2, AKT, and AS160. In murine and human cardiac tissue, nesfatin-1 levels varied with diet and coronary health. In conclusion, human and murine cardiomyocytes can synthesize and secrete nesfatin-1, which is able to induce glucose uptake and the mobilization of the glucose transporter GLUT-4 in these cells. Nesfatin-1 cardiac levels are regulated by diet and coronary health.
publishDate	2013
dc.date.none.fl_str_mv	2013
dc.type.none.fl_str_mv	info:eu-repo/semantics/article
format	article
dc.identifier.none.fl_str_mv	http://hdl.handle.net/20.500.11940/1982
url	http://hdl.handle.net/20.500.11940/1982
dc.language.none.fl_str_mv	Inglés
language_invalid_str_mv	Inglés
dc.rights.none.fl_str_mv	info:eu-repo/semantics/openAccess
eu_rights_str_mv	openAccess
dc.source.none.fl_str_mv	reponame:RUNA. Repositorio da Consellería de Sanidade e Sergas instname:Servizo Galego de Saúde (SERGAS)
instname_str	Servizo Galego de Saúde (SERGAS)
reponame_str	RUNA. Repositorio da Consellería de Sanidade e Sergas
collection	RUNA. Repositorio da Consellería de Sanidade e Sergas
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_	1869425214852431872
score	15,300719

Nesfatin-1 in human and murine cardiomyocytes: synthesis, secretion, and mobilization of GLUT-4

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