A role for Myosin in triggering and executing amnioserosa cell delaminations during dorsal closure

The remodeling of epithelial tissues is a critical process in morphogenesis, often involving the apoptotic removal of individual cells while preserving tissue integrity. In Drosophila, the amnioserosa—a highly dynamic extra-embryonic tissue—undergoes extensive remodeling, culminating in its complete...

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Detalles Bibliográficos
Autores: Gorfinkiel Haim, Nicole, Ferrer, Yanara, Recalde, Jon, Gutiérrez, Javier, Sáez, Guillermo
Tipo de recurso: artículo
Fecha de publicación:2025
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/125215
Acceso en línea:https://hdl.handle.net/20.500.14352/125215
Access Level:acceso abierto
Palabra clave:577.21
576
Cell delamination
Actomyosin contractility
Rho
Caspase activity
Biología celular (Biología)
Biología molecular (Biología)
2407 Biología Celular
2415 Biología Molecular
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oai_identifier_str oai:docta.ucm.es:20.500.14352/125215
network_acronym_str ES
network_name_str España
repository_id_str
spelling A role for Myosin in triggering and executing amnioserosa cell delaminations during dorsal closureGorfinkiel Haim, NicoleFerrer, YanaraRecalde, JonGutiérrez, JavierSáez, Guillermo577.21576Cell delaminationActomyosin contractilityRhoCaspase activityBiología celular (Biología)Biología molecular (Biología)2407 Biología Celular2415 Biología MolecularThe remodeling of epithelial tissues is a critical process in morphogenesis, often involving the apoptotic removal of individual cells while preserving tissue integrity. In Drosophila, the amnioserosa—a highly dynamic extra-embryonic tissue—undergoes extensive remodeling, culminating in its complete elimination at the end of dorsal closure. While apoptotic cell delaminations in the amnioserosa have been proposed to contribute to dorsal closure, the cellular mechanisms underlying this process remain poorly understood. In this study, we have investigated actomyosin dynamics during cell delaminations and analyzed the consequences of perturbing non-muscle Myosin activity globally in the entire tissue as well as locally in groups of cells. We found that Myosin plays an essential role in both triggering and executing cell delaminations, with high Myosin contractility promoting cell delamination via caspase activation. Additionally, our results suggest that cell delaminations are governed by both cell-autonomous Myosin dynamics and mechanical cues from the tissue environment. Together, these findings provide new insights into the regulation of epithelial cell removal and the complex interplay between apoptotic and mechanical signals during tissue remodeling.Nature ResearchUniversidad Complutense de Madrid20252025-09-0120252025-09-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/125215reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)InglésengAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 PID2020-114533GB-C22 MECANISMOS MECANOSENSIBLES EN LA COORDINACION DE LA ACTIVIDAD CELULAR DURANTE LA MORFOGENESIS EPITELIALopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/1252152026-06-02T12:44:21Z
dc.title.none.fl_str_mv A role for Myosin in triggering and executing amnioserosa cell delaminations during dorsal closure
title A role for Myosin in triggering and executing amnioserosa cell delaminations during dorsal closure
spellingShingle A role for Myosin in triggering and executing amnioserosa cell delaminations during dorsal closure
Gorfinkiel Haim, Nicole
577.21
576
Cell delamination
Actomyosin contractility
Rho
Caspase activity
Biología celular (Biología)
Biología molecular (Biología)
2407 Biología Celular
2415 Biología Molecular
title_short A role for Myosin in triggering and executing amnioserosa cell delaminations during dorsal closure
title_full A role for Myosin in triggering and executing amnioserosa cell delaminations during dorsal closure
title_fullStr A role for Myosin in triggering and executing amnioserosa cell delaminations during dorsal closure
title_full_unstemmed A role for Myosin in triggering and executing amnioserosa cell delaminations during dorsal closure
title_sort A role for Myosin in triggering and executing amnioserosa cell delaminations during dorsal closure
dc.creator.none.fl_str_mv Gorfinkiel Haim, Nicole
Ferrer, Yanara
Recalde, Jon
Gutiérrez, Javier
Sáez, Guillermo
author Gorfinkiel Haim, Nicole
author_facet Gorfinkiel Haim, Nicole
Ferrer, Yanara
Recalde, Jon
Gutiérrez, Javier
Sáez, Guillermo
author_role author
author2 Ferrer, Yanara
Recalde, Jon
Gutiérrez, Javier
Sáez, Guillermo
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 577.21
576
Cell delamination
Actomyosin contractility
Rho
Caspase activity
Biología celular (Biología)
Biología molecular (Biología)
2407 Biología Celular
2415 Biología Molecular
topic 577.21
576
Cell delamination
Actomyosin contractility
Rho
Caspase activity
Biología celular (Biología)
Biología molecular (Biología)
2407 Biología Celular
2415 Biología Molecular
description The remodeling of epithelial tissues is a critical process in morphogenesis, often involving the apoptotic removal of individual cells while preserving tissue integrity. In Drosophila, the amnioserosa—a highly dynamic extra-embryonic tissue—undergoes extensive remodeling, culminating in its complete elimination at the end of dorsal closure. While apoptotic cell delaminations in the amnioserosa have been proposed to contribute to dorsal closure, the cellular mechanisms underlying this process remain poorly understood. In this study, we have investigated actomyosin dynamics during cell delaminations and analyzed the consequences of perturbing non-muscle Myosin activity globally in the entire tissue as well as locally in groups of cells. We found that Myosin plays an essential role in both triggering and executing cell delaminations, with high Myosin contractility promoting cell delamination via caspase activation. Additionally, our results suggest that cell delaminations are governed by both cell-autonomous Myosin dynamics and mechanical cues from the tissue environment. Together, these findings provide new insights into the regulation of epithelial cell removal and the complex interplay between apoptotic and mechanical signals during tissue remodeling.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025-09-01
2025
2025-09-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/125215
url https://hdl.handle.net/20.500.14352/125215
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 PID2020-114533GB-C22 MECANISMOS MECANOSENSIBLES EN LA COORDINACION DE LA ACTIVIDAD CELULAR DURANTE LA MORFOGENESIS EPITELIAL
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Nature Research
publisher.none.fl_str_mv Nature Research
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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