Effect of silk fibroin microparticles on cellular immunity and liver of gilthead seabream (Sparus aurata L.) with and without experimental skin injuries

Silk fibroin microparticles (SF) were administered in the diet of gilthead seabream with or without experimental skin wounds to study the effects on cellular immunity and liver. A commercial diet was enriched with different SF contents: 0, 50 and 100 mg kg-1 (control, SF50 and SF100 diet, respective...

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Detalles Bibliográficos
Autores: Albaladejo-Riad, Nora, Espinosa-Ruiz, Cristóbal, Esteban Abad, María de los Ángeles
Tipo de recurso: conjunto de datos
Fecha de publicación:2023
País:España
Institución:Universidad de Murcia
Repositorio:DIGITUM. Depósito Digital Institucional de la Universidad de Murcia
OAI Identifier:oai:digitum.um.es:10201/131523
Acceso en línea:http://hdl.handle.net/10201/131523
Access Level:acceso abierto
Palabra clave:Gene expression
Leucocytes
Liver stress
Silk fibroin
Skin wound
Microscopy
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spelling Effect of silk fibroin microparticles on cellular immunity and liver of gilthead seabream (Sparus aurata L.) with and without experimental skin injuriesAlbaladejo-Riad, NoraEspinosa-Ruiz, CristóbalEsteban Abad, María de los ÁngelesGene expressionLeucocytesLiver stressSilk fibroinSkin woundMicroscopySilk fibroin microparticles (SF) were administered in the diet of gilthead seabream with or without experimental skin wounds to study the effects on cellular immunity and liver. A commercial diet was enriched with different SF contents: 0, 50 and 100 mg kg-1 (control, SF50 and SF100 diet, respectively). The animals were fed for 30 days and half of them were sampled. Similar experimental wounds were then performed to the rest of fish and they continued to be fed the same diet. At 7 days post-wounding, samples were taken from the wounded fish. Cellular immunity was studied on head kidney leucocytes (phagocytosis, respiratory and peroxidase content) and liver status (histological study and gene expression) were studied. Our results showed that experimental wounds affect both cellular immunity (by decreasing leucocyte respiratory burst and peroxidase activity) and altered liver histology (by inducing vascularization and congestion of blood vessels). In addition, it affects the expression of genes indicative of oxidative stress, endoplasmic reticulum stress and apoptosis. More specifically, it was the highest dose of silk fibroin (SF100) that significantly increased the phagocytic capacity of leucocytes as well as vascularization in the liver. In addition, increased expression of antioxidant genes (cat and gsr) and decreased expression of genes related to reticulum endoplasmic stress (grp94 and grp170) and apoptosis (nos and jnk) were detected in these fish fed with SF100 and wounded. In conclusion, fed fish with SF100 had many beneficial effects as cellular immunostimulant and hepatoprotection in wounded fish. Its use could be of great interest for stress management in farmed fish conditions.ElsevierBiología Celular e Histología20232023info:eu-repo/semantics/datasetapplication/vnd.ms-excel2application/vnd.ms-excelhttp://hdl.handle.net/10201/131523reponame:DIGITUM. Depósito Digital Institucional de la Universidad de Murciainstname:Universidad de MurciaInglésSilk fibroin microparticles were kindly provided by Prof. G. Víllora and G. Díaz-Baños (Department of Chemical Engineering, Faculty of Chemistry, University of Murcia, Spain). N.A.R. is pursuing her Ph.D. degree with a scholarship granted by the MINECO (FPU18/02544). This work was supported by the MINECO co-funded by the European Regional Development Funds (ERDF/FEDER) (grant no. AGL2017-88370-C3-1-R) and is part of the ThinkInAzul programme supported by MCIN with funding from European Union Next Generation EU (PRTR-C17.I1) and by the Comunidad Autónoma de la Región de Murcia-Fundación Séneca (Spain).info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0/oai:digitum.um.es:10201/1315232026-05-27T12:40:41Z
dc.title.none.fl_str_mv Effect of silk fibroin microparticles on cellular immunity and liver of gilthead seabream (Sparus aurata L.) with and without experimental skin injuries
title Effect of silk fibroin microparticles on cellular immunity and liver of gilthead seabream (Sparus aurata L.) with and without experimental skin injuries
spellingShingle Effect of silk fibroin microparticles on cellular immunity and liver of gilthead seabream (Sparus aurata L.) with and without experimental skin injuries
Albaladejo-Riad, Nora
Gene expression
Leucocytes
Liver stress
Silk fibroin
Skin wound
Microscopy
title_short Effect of silk fibroin microparticles on cellular immunity and liver of gilthead seabream (Sparus aurata L.) with and without experimental skin injuries
title_full Effect of silk fibroin microparticles on cellular immunity and liver of gilthead seabream (Sparus aurata L.) with and without experimental skin injuries
title_fullStr Effect of silk fibroin microparticles on cellular immunity and liver of gilthead seabream (Sparus aurata L.) with and without experimental skin injuries
title_full_unstemmed Effect of silk fibroin microparticles on cellular immunity and liver of gilthead seabream (Sparus aurata L.) with and without experimental skin injuries
title_sort Effect of silk fibroin microparticles on cellular immunity and liver of gilthead seabream (Sparus aurata L.) with and without experimental skin injuries
dc.creator.none.fl_str_mv Albaladejo-Riad, Nora
Espinosa-Ruiz, Cristóbal
Esteban Abad, María de los Ángeles
author Albaladejo-Riad, Nora
author_facet Albaladejo-Riad, Nora
Espinosa-Ruiz, Cristóbal
Esteban Abad, María de los Ángeles
author_role author
author2 Espinosa-Ruiz, Cristóbal
Esteban Abad, María de los Ángeles
author2_role author
author
dc.contributor.none.fl_str_mv Biología Celular e Histología
dc.subject.none.fl_str_mv Gene expression
Leucocytes
Liver stress
Silk fibroin
Skin wound
Microscopy
topic Gene expression
Leucocytes
Liver stress
Silk fibroin
Skin wound
Microscopy
description Silk fibroin microparticles (SF) were administered in the diet of gilthead seabream with or without experimental skin wounds to study the effects on cellular immunity and liver. A commercial diet was enriched with different SF contents: 0, 50 and 100 mg kg-1 (control, SF50 and SF100 diet, respectively). The animals were fed for 30 days and half of them were sampled. Similar experimental wounds were then performed to the rest of fish and they continued to be fed the same diet. At 7 days post-wounding, samples were taken from the wounded fish. Cellular immunity was studied on head kidney leucocytes (phagocytosis, respiratory and peroxidase content) and liver status (histological study and gene expression) were studied. Our results showed that experimental wounds affect both cellular immunity (by decreasing leucocyte respiratory burst and peroxidase activity) and altered liver histology (by inducing vascularization and congestion of blood vessels). In addition, it affects the expression of genes indicative of oxidative stress, endoplasmic reticulum stress and apoptosis. More specifically, it was the highest dose of silk fibroin (SF100) that significantly increased the phagocytic capacity of leucocytes as well as vascularization in the liver. In addition, increased expression of antioxidant genes (cat and gsr) and decreased expression of genes related to reticulum endoplasmic stress (grp94 and grp170) and apoptosis (nos and jnk) were detected in these fish fed with SF100 and wounded. In conclusion, fed fish with SF100 had many beneficial effects as cellular immunostimulant and hepatoprotection in wounded fish. Its use could be of great interest for stress management in farmed fish conditions.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/dataset
format dataset
dc.identifier.none.fl_str_mv http://hdl.handle.net/10201/131523
url http://hdl.handle.net/10201/131523
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Silk fibroin microparticles were kindly provided by Prof. G. Víllora and G. Díaz-Baños (Department of Chemical Engineering, Faculty of Chemistry, University of Murcia, Spain). N.A.R. is pursuing her Ph.D. degree with a scholarship granted by the MINECO (FPU18/02544). This work was supported by the MINECO co-funded by the European Regional Development Funds (ERDF/FEDER) (grant no. AGL2017-88370-C3-1-R) and is part of the ThinkInAzul programme supported by MCIN with funding from European Union Next Generation EU (PRTR-C17.I1) and by the Comunidad Autónoma de la Región de Murcia-Fundación Séneca (Spain).
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.format.none.fl_str_mv application/vnd.ms-excel
2
application/vnd.ms-excel
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITUM. Depósito Digital Institucional de la Universidad de Murcia
instname:Universidad de Murcia
instname_str Universidad de Murcia
reponame_str DIGITUM. Depósito Digital Institucional de la Universidad de Murcia
collection DIGITUM. Depósito Digital Institucional de la Universidad de Murcia
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.301603

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