Fluoxetine increased adult neurogenesis is mediated by 5-HT3 receptor

Adult neurogenesis is an aspect of structural plasticity that remains active during adulthood in some brain regions. One of them is the subgranular zone (SGZ) of the dentate gyrus of the hippocampus. Adult neurogenesis is reduced by different factors and in disorders of the CNS, including major depr...

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Autores: Olivas-Cano, I, Rodriguez-Andreu, J M, Blasco-Ibanez, J M, Crespo, C, Nacher, J, Varea, E
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:INCLIVA
Repositorio:r-INCLIVA. Repositorio Institucional de Producción Científica de INCLIVA
OAI Identifier:oai:incliva.fundanetsuite.com:p17113
Acceso en línea:https://incliva.portalinvestigacion.com/publicaciones/17113
Access Level:acceso abierto
Palabra clave:Serotonin receptor 3
Adult neurogenesis
Fluoxetine
Ondansetron
Hippocampus
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spelling Fluoxetine increased adult neurogenesis is mediated by 5-HT3 receptorOlivas-Cano, IRodriguez-Andreu, J MBlasco-Ibanez, J MCrespo, CNacher, JVarea, ESerotonin receptor 3Adult neurogenesisFluoxetineOndansetronHippocampusAdult neurogenesis is an aspect of structural plasticity that remains active during adulthood in some brain regions. One of them is the subgranular zone (SGZ) of the dentate gyrus of the hippocampus. Adult neurogenesis is reduced by different factors and in disorders of the CNS, including major depression. Antidepressant treatments, such as chronic fluoxetine administration, recover the normal level of adult neurogenesis. Fluoxetine treatment increases the free concentration of the neurotransmitter serotonin and this monoamine is implicated in the regulation of the neurogenic process; however, the target of the action of this neurotransmitter has not been fully elucidated. In this study, we have tried to determine the relevance of the serotonin receptor 3 (5-HT3) in the hippocampal neurogenesis of adult rats. We have used fluorescent immunohistochemistry to study the expression of the 5-HT3 receptor in different neurogenesis stages in the SGZ, identifying its expression in stem cells, amplifying neural progenitors and immature neurons. Moreover, we have studied the impact of a 5-HT3 antagonist (ondansetron) in the fluoxetine-induced adult neurogenesis. We observed that fluoxetine alone increases the number of both proliferating cells (ki67 positive) and immature neurons (DCX positive) in the SGZ. By contrast, co-treatment with ondansetron blocked the increase in proliferation and neurogenesis. This study demonstrates that the activation of 5-HT3 receptors is necessary for the increase of adult neurogenesis induced by fluoxetine.ELSEVIER IRELAND LTD2023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://incliva.portalinvestigacion.com/publicaciones/17113NEUROSCIENCE LETTERSISSN: 03043940ISSNe: 18727972reponame:r-INCLIVA. Repositorio Institucional de Producción Científica de INCLIVAinstname:INCLIVAInglésinfo:eu-repo/semantics/openAccessoai:incliva.fundanetsuite.com:p171132026-06-07T16:35:31Z
dc.title.none.fl_str_mv Fluoxetine increased adult neurogenesis is mediated by 5-HT3 receptor
title Fluoxetine increased adult neurogenesis is mediated by 5-HT3 receptor
spellingShingle Fluoxetine increased adult neurogenesis is mediated by 5-HT3 receptor
Olivas-Cano, I
Serotonin receptor 3
Adult neurogenesis
Fluoxetine
Ondansetron
Hippocampus
title_short Fluoxetine increased adult neurogenesis is mediated by 5-HT3 receptor
title_full Fluoxetine increased adult neurogenesis is mediated by 5-HT3 receptor
title_fullStr Fluoxetine increased adult neurogenesis is mediated by 5-HT3 receptor
title_full_unstemmed Fluoxetine increased adult neurogenesis is mediated by 5-HT3 receptor
title_sort Fluoxetine increased adult neurogenesis is mediated by 5-HT3 receptor
dc.creator.none.fl_str_mv Olivas-Cano, I
Rodriguez-Andreu, J M
Blasco-Ibanez, J M
Crespo, C
Nacher, J
Varea, E
author Olivas-Cano, I
author_facet Olivas-Cano, I
Rodriguez-Andreu, J M
Blasco-Ibanez, J M
Crespo, C
Nacher, J
Varea, E
author_role author
author2 Rodriguez-Andreu, J M
Blasco-Ibanez, J M
Crespo, C
Nacher, J
Varea, E
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Serotonin receptor 3
Adult neurogenesis
Fluoxetine
Ondansetron
Hippocampus
topic Serotonin receptor 3
Adult neurogenesis
Fluoxetine
Ondansetron
Hippocampus
description Adult neurogenesis is an aspect of structural plasticity that remains active during adulthood in some brain regions. One of them is the subgranular zone (SGZ) of the dentate gyrus of the hippocampus. Adult neurogenesis is reduced by different factors and in disorders of the CNS, including major depression. Antidepressant treatments, such as chronic fluoxetine administration, recover the normal level of adult neurogenesis. Fluoxetine treatment increases the free concentration of the neurotransmitter serotonin and this monoamine is implicated in the regulation of the neurogenic process; however, the target of the action of this neurotransmitter has not been fully elucidated. In this study, we have tried to determine the relevance of the serotonin receptor 3 (5-HT3) in the hippocampal neurogenesis of adult rats. We have used fluorescent immunohistochemistry to study the expression of the 5-HT3 receptor in different neurogenesis stages in the SGZ, identifying its expression in stem cells, amplifying neural progenitors and immature neurons. Moreover, we have studied the impact of a 5-HT3 antagonist (ondansetron) in the fluoxetine-induced adult neurogenesis. We observed that fluoxetine alone increases the number of both proliferating cells (ki67 positive) and immature neurons (DCX positive) in the SGZ. By contrast, co-treatment with ondansetron blocked the increase in proliferation and neurogenesis. This study demonstrates that the activation of 5-HT3 receptors is necessary for the increase of adult neurogenesis induced by fluoxetine.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://incliva.portalinvestigacion.com/publicaciones/17113
url https://incliva.portalinvestigacion.com/publicaciones/17113
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv ELSEVIER IRELAND LTD
publisher.none.fl_str_mv ELSEVIER IRELAND LTD
dc.source.none.fl_str_mv NEUROSCIENCE LETTERS
ISSN: 03043940
ISSNe: 18727972
reponame:r-INCLIVA. Repositorio Institucional de Producción Científica de INCLIVA
instname:INCLIVA
instname_str INCLIVA
reponame_str r-INCLIVA. Repositorio Institucional de Producción Científica de INCLIVA
collection r-INCLIVA. Repositorio Institucional de Producción Científica de INCLIVA
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repository.mail.fl_str_mv
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