Micro-matrix solid-phase dispersion method for the multiresidue determination of tetracycline antibiotics and their transformation products in radish
Parent tetracyclines and their transformation products can be present in vegetables growth in soils amended with manure, treated sewage sludge, or irrigated with reclaimed urban wastewater. The presence of some tetracycline antibiotics and transformation products is already regulated in foodstuffs o...
| Autores: | , , , , |
|---|---|
| Tipo de documento: | artigo |
| Estado: | Versão publicada |
| Data de publicação: | 2026 |
| País: | España |
| Recursos: | Universidad de Sevilla (US) |
| Repositório: | idUS. Depósito de Investigación de la Universidad de Sevilla |
| OAI Identifier: | oai:dnet:idus________::3e537d942700c0fb382aa7f71f9ae451 |
| Acesso em linha: | https://hdl.handle.net/11441/185113 https://doi.org/10.1016/j.microc.2026.117682 |
| Access Level: | Acceso aberto |
| Palavra-chave: | μ-MSPD Online SPE LC-MS/MS Epimers Greenness assessment ComplexGAPI AGREEprep |
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Micro-matrix solid-phase dispersion method for the multiresidue determination of tetracycline antibiotics and their transformation products in radishGarcía-Criado, NoeliaMartín Bueno, JuliaSantos Morcillo, Juan LuisAparicio Gómez, IreneAlonso Álvarez, Estebanμ-MSPDOnline SPELC-MS/MSEpimersGreenness assessmentComplexGAPIAGREEprepParent tetracyclines and their transformation products can be present in vegetables growth in soils amended with manure, treated sewage sludge, or irrigated with reclaimed urban wastewater. The presence of some tetracycline antibiotics and transformation products is already regulated in foodstuffs of animal origin but not yet in vegetables. In this paper, an analytical method is proposed for the determination of seven parent tetracycline antibiotics and six transformation products. Selected transformation products correspond to epimers and dehydration products, which have been reported to be the most toxic ones. The proposed method is based on miniaturized matrix solid-phase dispersion (μ-MSPD) method combined with online solid-phase extraction–liquid chromatography–tandem mass spectrometry (online SPE–LC–MS/MS) determination. The method has been validated in radish edible parts (leaves and bulb), to be used for food monitoring, and in roots, to be used in pollutant plant uptake and translocation studies. Proposed method provides MQLs lower than 5ngg⁻¹ dw, accuracy in the range 85–100%, and RSD% lower than 5.5%, for most of the compounds and matrices. Application of the method to radish samples revealed the presence of transformation products of tetracycline antibiotics even in samples where their respective parent compound was not detected. Greenness assessment, using ComplexGAPI and AGREEprep Green Analytical Chemistry metrics, revealed the highest greenness degree of the proposed method in comparison to other methods reported in literature for determination of tetracycline antibiotics in vegetables.ElsevierQuímica AnalíticaFQM344: Análisis Químico Industrial y MedioambientalMinisterio de Ciencia e Innovación (MICIN). EspañaMinisterio de Ciencia, Innovación y Universidades (MICIU). España2026info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/185113https://doi.org/10.1016/j.microc.2026.117682reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésMicrochemical Journal, 224, 117682. PID2020-117641RB-I00PRE2021–100799https://www.sciencedirect.com/science/article/pii/S0026265X26008842info:eu-repo/semantics/openAccessoai:dnet:idus________::3e537d942700c0fb382aa7f71f9ae4512026-06-17T12:51:07Z |
| dc.title.none.fl_str_mv |
Micro-matrix solid-phase dispersion method for the multiresidue determination of tetracycline antibiotics and their transformation products in radish |
| title |
Micro-matrix solid-phase dispersion method for the multiresidue determination of tetracycline antibiotics and their transformation products in radish |
| spellingShingle |
Micro-matrix solid-phase dispersion method for the multiresidue determination of tetracycline antibiotics and their transformation products in radish García-Criado, Noelia μ-MSPD Online SPE LC-MS/MS Epimers Greenness assessment ComplexGAPI AGREEprep |
| title_short |
Micro-matrix solid-phase dispersion method for the multiresidue determination of tetracycline antibiotics and their transformation products in radish |
| title_full |
Micro-matrix solid-phase dispersion method for the multiresidue determination of tetracycline antibiotics and their transformation products in radish |
| title_fullStr |
Micro-matrix solid-phase dispersion method for the multiresidue determination of tetracycline antibiotics and their transformation products in radish |
| title_full_unstemmed |
Micro-matrix solid-phase dispersion method for the multiresidue determination of tetracycline antibiotics and their transformation products in radish |
| title_sort |
Micro-matrix solid-phase dispersion method for the multiresidue determination of tetracycline antibiotics and their transformation products in radish |
| dc.creator.none.fl_str_mv |
García-Criado, Noelia Martín Bueno, Julia Santos Morcillo, Juan Luis Aparicio Gómez, Irene Alonso Álvarez, Esteban |
| author |
García-Criado, Noelia |
| author_facet |
García-Criado, Noelia Martín Bueno, Julia Santos Morcillo, Juan Luis Aparicio Gómez, Irene Alonso Álvarez, Esteban |
| author_role |
author |
| author2 |
Martín Bueno, Julia Santos Morcillo, Juan Luis Aparicio Gómez, Irene Alonso Álvarez, Esteban |
| author2_role |
author author author author |
| dc.contributor.none.fl_str_mv |
Química Analítica FQM344: Análisis Químico Industrial y Medioambiental Ministerio de Ciencia e Innovación (MICIN). España Ministerio de Ciencia, Innovación y Universidades (MICIU). España |
| dc.subject.none.fl_str_mv |
μ-MSPD Online SPE LC-MS/MS Epimers Greenness assessment ComplexGAPI AGREEprep |
| topic |
μ-MSPD Online SPE LC-MS/MS Epimers Greenness assessment ComplexGAPI AGREEprep |
| description |
Parent tetracyclines and their transformation products can be present in vegetables growth in soils amended with manure, treated sewage sludge, or irrigated with reclaimed urban wastewater. The presence of some tetracycline antibiotics and transformation products is already regulated in foodstuffs of animal origin but not yet in vegetables. In this paper, an analytical method is proposed for the determination of seven parent tetracycline antibiotics and six transformation products. Selected transformation products correspond to epimers and dehydration products, which have been reported to be the most toxic ones. The proposed method is based on miniaturized matrix solid-phase dispersion (μ-MSPD) method combined with online solid-phase extraction–liquid chromatography–tandem mass spectrometry (online SPE–LC–MS/MS) determination. The method has been validated in radish edible parts (leaves and bulb), to be used for food monitoring, and in roots, to be used in pollutant plant uptake and translocation studies. Proposed method provides MQLs lower than 5ngg⁻¹ dw, accuracy in the range 85–100%, and RSD% lower than 5.5%, for most of the compounds and matrices. Application of the method to radish samples revealed the presence of transformation products of tetracycline antibiotics even in samples where their respective parent compound was not detected. Greenness assessment, using ComplexGAPI and AGREEprep Green Analytical Chemistry metrics, revealed the highest greenness degree of the proposed method in comparison to other methods reported in literature for determination of tetracycline antibiotics in vegetables. |
| publishDate |
2026 |
| dc.date.none.fl_str_mv |
2026 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/11441/185113 https://doi.org/10.1016/j.microc.2026.117682 |
| url |
https://hdl.handle.net/11441/185113 https://doi.org/10.1016/j.microc.2026.117682 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
Microchemical Journal, 224, 117682. PID2020-117641RB-I00 PRE2021–100799 https://www.sciencedirect.com/science/article/pii/S0026265X26008842 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf application/pdf |
| dc.publisher.none.fl_str_mv |
Elsevier |
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Elsevier |
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reponame:idUS. Depósito de Investigación de la Universidad de Sevilla instname:Universidad de Sevilla (US) |
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Universidad de Sevilla (US) |
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idUS. Depósito de Investigación de la Universidad de Sevilla |
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idUS. Depósito de Investigación de la Universidad de Sevilla |
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15,811543 |