Detection of tetrodotoxins in juvenile pufferfish Lagocephalus sceleratus (Gmelin, 1789) from the North Aegean Sea (Greece) by an electrochemical magnetic bead-based immunosensing tool

Two small Lagocephalus sceleratus juveniles were captured in picarel targeting catches from North Aegean Sea (Greece) in the autumn of 2017. An electrochemical immunosensing tool using magnetic beads as immobilisation support was developed and applied to the rapid screening of tetrodotoxins (TTXs),...

Descripción completa

Detalles Bibliográficos
Autores: Leonardo, Sandra, Kiparissis, Sotiris, Rambla-Alegre, Maria, Almarza, Sergi, Roque, Ana, Andrée, Karl Blyth, Christidis, Aristidis, Flores, Cintia, Caixach, Josep, Campbell, Katrina, Elliott, Christopher T. T., Aligizaki, Katerina, Diogène, Jorge, Campàs, Mònica
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2019
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/201952
Acceso en línea:http://hdl.handle.net/10261/201952
Access Level:acceso abierto
Palabra clave:Juvenile
Electrochemical
Immunoassay
Lagocephalus sceleratus
Liquid chromatography-high resolution mass spectrometry (LC-HRMS)
Tetrodotoxin
Magnetic bead
Pufferfish
http://metadata.un.org/sdg/4
Ensure inclusive and equitable quality education and promote lifelong learning opportunities for all
id ES_fa144a7e664ff00ef8fbbbe292644b17
oai_identifier_str oai:digital.csic.es:10261/201952
network_acronym_str ES
network_name_str España
repository_id_str
spelling Detection of tetrodotoxins in juvenile pufferfish Lagocephalus sceleratus (Gmelin, 1789) from the North Aegean Sea (Greece) by an electrochemical magnetic bead-based immunosensing toolLeonardo, SandraKiparissis, SotirisRambla-Alegre, MariaAlmarza, SergiRoque, AnaAndrée, Karl BlythChristidis, AristidisFlores, CintiaCaixach, JosepCampbell, KatrinaElliott, Christopher T. T.Aligizaki, KaterinaDiogène, JorgeCampàs, MònicaJuvenileElectrochemicalImmunoassayLagocephalus sceleratusLiquid chromatography-high resolution mass spectrometry (LC-HRMS)TetrodotoxinMagnetic beadPufferfishhttp://metadata.un.org/sdg/4Ensure inclusive and equitable quality education and promote lifelong learning opportunities for allTwo small Lagocephalus sceleratus juveniles were captured in picarel targeting catches from North Aegean Sea (Greece) in the autumn of 2017. An electrochemical immunosensing tool using magnetic beads as immobilisation support was developed and applied to the rapid screening of tetrodotoxins (TTXs), potent neurotoxins that constitute a food safety hazard when present in seafood. This tool revealed the presence of TTXs in both individuals. Results were compared with those provided by mELISA and LC-HRMS, the latter confirming the presence of TTX. Some of the tissues contained TTX contents close to or above 2 mg/kg. L. sceleratus juveniles had been considered as non-toxic and, to our knowledge, this is the first report of high TTX levels in small L. sceleratus individuals. Such specimens can be mistaken with other edible species, posing a threat to consumers. The availability of low-cost and user-friendly tools for TTXs detection will contribute to guarantee seafood safety. © 2019 Elsevier LtdThe research leading to these results has received funding from the Ministerio de Ciencia, Innovación y Universidades through the CIGUASENSING (BIO2017-87946-C2-2-R) project. The authors wish to express their gratitude to Dimitris Lachouvaris for collecting the samples and to Dr Nikolaos Lambadariou for providing the map. The authors also acknowledge support from CERCA Programme/Generalitat de Catalunya.Peer reviewedElsevierFlores, Cintia [0000-0002-7766-5639]Caixach, Josep [0000-0003-2326-9443]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202020202019info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/201952reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.1016/j.foodchem.2019.03.148Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2019522026-05-22T06:33:51Z
dc.title.none.fl_str_mv Detection of tetrodotoxins in juvenile pufferfish Lagocephalus sceleratus (Gmelin, 1789) from the North Aegean Sea (Greece) by an electrochemical magnetic bead-based immunosensing tool
title Detection of tetrodotoxins in juvenile pufferfish Lagocephalus sceleratus (Gmelin, 1789) from the North Aegean Sea (Greece) by an electrochemical magnetic bead-based immunosensing tool
spellingShingle Detection of tetrodotoxins in juvenile pufferfish Lagocephalus sceleratus (Gmelin, 1789) from the North Aegean Sea (Greece) by an electrochemical magnetic bead-based immunosensing tool
Leonardo, Sandra
Juvenile
Electrochemical
Immunoassay
Lagocephalus sceleratus
Liquid chromatography-high resolution mass spectrometry (LC-HRMS)
Tetrodotoxin
Magnetic bead
Pufferfish
http://metadata.un.org/sdg/4
Ensure inclusive and equitable quality education and promote lifelong learning opportunities for all
title_short Detection of tetrodotoxins in juvenile pufferfish Lagocephalus sceleratus (Gmelin, 1789) from the North Aegean Sea (Greece) by an electrochemical magnetic bead-based immunosensing tool
title_full Detection of tetrodotoxins in juvenile pufferfish Lagocephalus sceleratus (Gmelin, 1789) from the North Aegean Sea (Greece) by an electrochemical magnetic bead-based immunosensing tool
title_fullStr Detection of tetrodotoxins in juvenile pufferfish Lagocephalus sceleratus (Gmelin, 1789) from the North Aegean Sea (Greece) by an electrochemical magnetic bead-based immunosensing tool
title_full_unstemmed Detection of tetrodotoxins in juvenile pufferfish Lagocephalus sceleratus (Gmelin, 1789) from the North Aegean Sea (Greece) by an electrochemical magnetic bead-based immunosensing tool
title_sort Detection of tetrodotoxins in juvenile pufferfish Lagocephalus sceleratus (Gmelin, 1789) from the North Aegean Sea (Greece) by an electrochemical magnetic bead-based immunosensing tool
dc.creator.none.fl_str_mv Leonardo, Sandra
Kiparissis, Sotiris
Rambla-Alegre, Maria
Almarza, Sergi
Roque, Ana
Andrée, Karl Blyth
Christidis, Aristidis
Flores, Cintia
Caixach, Josep
Campbell, Katrina
Elliott, Christopher T. T.
Aligizaki, Katerina
Diogène, Jorge
Campàs, Mònica
author Leonardo, Sandra
author_facet Leonardo, Sandra
Kiparissis, Sotiris
Rambla-Alegre, Maria
Almarza, Sergi
Roque, Ana
Andrée, Karl Blyth
Christidis, Aristidis
Flores, Cintia
Caixach, Josep
Campbell, Katrina
Elliott, Christopher T. T.
Aligizaki, Katerina
Diogène, Jorge
Campàs, Mònica
author_role author
author2 Kiparissis, Sotiris
Rambla-Alegre, Maria
Almarza, Sergi
Roque, Ana
Andrée, Karl Blyth
Christidis, Aristidis
Flores, Cintia
Caixach, Josep
Campbell, Katrina
Elliott, Christopher T. T.
Aligizaki, Katerina
Diogène, Jorge
Campàs, Mònica
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Flores, Cintia [0000-0002-7766-5639]
Caixach, Josep [0000-0003-2326-9443]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Juvenile
Electrochemical
Immunoassay
Lagocephalus sceleratus
Liquid chromatography-high resolution mass spectrometry (LC-HRMS)
Tetrodotoxin
Magnetic bead
Pufferfish
http://metadata.un.org/sdg/4
Ensure inclusive and equitable quality education and promote lifelong learning opportunities for all
topic Juvenile
Electrochemical
Immunoassay
Lagocephalus sceleratus
Liquid chromatography-high resolution mass spectrometry (LC-HRMS)
Tetrodotoxin
Magnetic bead
Pufferfish
http://metadata.un.org/sdg/4
Ensure inclusive and equitable quality education and promote lifelong learning opportunities for all
description Two small Lagocephalus sceleratus juveniles were captured in picarel targeting catches from North Aegean Sea (Greece) in the autumn of 2017. An electrochemical immunosensing tool using magnetic beads as immobilisation support was developed and applied to the rapid screening of tetrodotoxins (TTXs), potent neurotoxins that constitute a food safety hazard when present in seafood. This tool revealed the presence of TTXs in both individuals. Results were compared with those provided by mELISA and LC-HRMS, the latter confirming the presence of TTX. Some of the tissues contained TTX contents close to or above 2 mg/kg. L. sceleratus juveniles had been considered as non-toxic and, to our knowledge, this is the first report of high TTX levels in small L. sceleratus individuals. Such specimens can be mistaken with other edible species, posing a threat to consumers. The availability of low-cost and user-friendly tools for TTXs detection will contribute to guarantee seafood safety. © 2019 Elsevier Ltd
publishDate 2019
dc.date.none.fl_str_mv 2019
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/201952
url http://hdl.handle.net/10261/201952
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1016/j.foodchem.2019.03.148
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869425152008126464
score 15,811543

Similares en LA Referencia