Effects of liposomal formulation of citicoline in experimental diabetes-induced retinal neurodegeneration

Diabetic retinopathy (DR) has been classically considered a microcirculatory disease of the retina. However, there is growing evidence to suggest that retinal neurodegeneration is also an early event in the pathogenesis of DR. Citicoline has been successfully used as a neuroprotective agent in the t...

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Autores: Bogdanov, Patricia|||0000-0003-0669-4958, Sampedro, Joel|||0000-0002-8463-7740, Solà-Adell, Cristina, Simó-Servat, Olga|||0000-0003-3483-652X, Russo, Carla, Varela-Sende, Luisa, Simó Canonge, Rafael|||0000-0003-0475-3096, Hernández, Cristina|||0000-0002-3109-1721
Tipo de recurso: artículo
Fecha de publicación:2018
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:201109
Acceso en línea:https://ddd.uab.cat/record/201109
https://dx.doi.org/urn:doi:10.3390/ijms19082458
Access Level:acceso abierto
Palabra clave:Citicoline
Diabetic retinopathy
Retinal neurodegeneration
Db/db mouse
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spelling Effects of liposomal formulation of citicoline in experimental diabetes-induced retinal neurodegenerationBogdanov, Patricia|||0000-0003-0669-4958Sampedro, Joel|||0000-0002-8463-7740Solà-Adell, CristinaSimó-Servat, Olga|||0000-0003-3483-652XRusso, CarlaVarela-Sende, LuisaSimó Canonge, Rafael|||0000-0003-0475-3096Hernández, Cristina|||0000-0002-3109-1721CiticolineDiabetic retinopathyRetinal neurodegenerationDb/db mouseDiabetic retinopathy (DR) has been classically considered a microcirculatory disease of the retina. However, there is growing evidence to suggest that retinal neurodegeneration is also an early event in the pathogenesis of DR. Citicoline has been successfully used as a neuroprotective agent in the treatment of glaucoma but their effects on DR remain to be elucidated. On this basis, the main aim of the present study was to evaluate the effect of topical administration of citicoline in liposomal formulation on retinal neurodegeneration in db/db mouse and to investigate the underlying mechanisms of action. The treatment (citicoline or vehicle) was topically administered twice daily for 15 days. Retinal analyses were performed in vivo by electroretinography and ex vivo by using Western blot and immunofluorescence measurements. We found that the liposomal formulation of citicoline prevented glial activation and neural apoptosis in the diabetic retina. The main mechanism implicated in these beneficial effects were the inhibition of the downregulation of synaptophysin and its anti-inflammatory properties by means of preventing the upregulation of NF-κB and TNF-α (Tumor Necrosis Factor α) induced by diabetes. Overall, these results suggest that topical administration of citicoline in liposomal formulation could be considered as a new strategy for treating the early stages of DR. 22018-01-0120182018-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/201109https://dx.doi.org/urn:doi:10.3390/ijms19082458reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2011092026-06-06T12:50:31Z
dc.title.none.fl_str_mv Effects of liposomal formulation of citicoline in experimental diabetes-induced retinal neurodegeneration
title Effects of liposomal formulation of citicoline in experimental diabetes-induced retinal neurodegeneration
spellingShingle Effects of liposomal formulation of citicoline in experimental diabetes-induced retinal neurodegeneration
Bogdanov, Patricia|||0000-0003-0669-4958
Citicoline
Diabetic retinopathy
Retinal neurodegeneration
Db/db mouse
title_short Effects of liposomal formulation of citicoline in experimental diabetes-induced retinal neurodegeneration
title_full Effects of liposomal formulation of citicoline in experimental diabetes-induced retinal neurodegeneration
title_fullStr Effects of liposomal formulation of citicoline in experimental diabetes-induced retinal neurodegeneration
title_full_unstemmed Effects of liposomal formulation of citicoline in experimental diabetes-induced retinal neurodegeneration
title_sort Effects of liposomal formulation of citicoline in experimental diabetes-induced retinal neurodegeneration
dc.creator.none.fl_str_mv Bogdanov, Patricia|||0000-0003-0669-4958
Sampedro, Joel|||0000-0002-8463-7740
Solà-Adell, Cristina
Simó-Servat, Olga|||0000-0003-3483-652X
Russo, Carla
Varela-Sende, Luisa
Simó Canonge, Rafael|||0000-0003-0475-3096
Hernández, Cristina|||0000-0002-3109-1721
author Bogdanov, Patricia|||0000-0003-0669-4958
author_facet Bogdanov, Patricia|||0000-0003-0669-4958
Sampedro, Joel|||0000-0002-8463-7740
Solà-Adell, Cristina
Simó-Servat, Olga|||0000-0003-3483-652X
Russo, Carla
Varela-Sende, Luisa
Simó Canonge, Rafael|||0000-0003-0475-3096
Hernández, Cristina|||0000-0002-3109-1721
author_role author
author2 Sampedro, Joel|||0000-0002-8463-7740
Solà-Adell, Cristina
Simó-Servat, Olga|||0000-0003-3483-652X
Russo, Carla
Varela-Sende, Luisa
Simó Canonge, Rafael|||0000-0003-0475-3096
Hernández, Cristina|||0000-0002-3109-1721
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Citicoline
Diabetic retinopathy
Retinal neurodegeneration
Db/db mouse
topic Citicoline
Diabetic retinopathy
Retinal neurodegeneration
Db/db mouse
description Diabetic retinopathy (DR) has been classically considered a microcirculatory disease of the retina. However, there is growing evidence to suggest that retinal neurodegeneration is also an early event in the pathogenesis of DR. Citicoline has been successfully used as a neuroprotective agent in the treatment of glaucoma but their effects on DR remain to be elucidated. On this basis, the main aim of the present study was to evaluate the effect of topical administration of citicoline in liposomal formulation on retinal neurodegeneration in db/db mouse and to investigate the underlying mechanisms of action. The treatment (citicoline or vehicle) was topically administered twice daily for 15 days. Retinal analyses were performed in vivo by electroretinography and ex vivo by using Western blot and immunofluorescence measurements. We found that the liposomal formulation of citicoline prevented glial activation and neural apoptosis in the diabetic retina. The main mechanism implicated in these beneficial effects were the inhibition of the downregulation of synaptophysin and its anti-inflammatory properties by means of preventing the upregulation of NF-κB and TNF-α (Tumor Necrosis Factor α) induced by diabetes. Overall, these results suggest that topical administration of citicoline in liposomal formulation could be considered as a new strategy for treating the early stages of DR.
publishDate 2018
dc.date.none.fl_str_mv 2
2018-01-01
2018
2018-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/201109
https://dx.doi.org/urn:doi:10.3390/ijms19082458
url https://ddd.uab.cat/record/201109
https://dx.doi.org/urn:doi:10.3390/ijms19082458
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
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