Crystallization and preliminary crystallographic characterization of the extrinsic PsbP protein of photosystem II from Spinacia oleracea

Preliminary X-ray diffraction analysis of the extrinsic PsbP protein of photosystem II from spinach (Spinacia oleracea) was performed using N-terminally His-tagged recombinant PsbP protein overexpressed in Escherichia coli. Recombinant PsbP protein (thrombin-digested recombinant His-tagged PsbP) sto...

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Autores: Kohoutova, Jaroslava, Kuta-Smatanova, Ivana, Brynda, Jiri, Lapkouski, Mikalai, Revuelta Doval, José Luis, Arellano, Juan B., Ettrich, Rüdiger
Tipo de recurso: artículo
Fecha de publicación:2009
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/20713
Acceso en línea:http://hdl.handle.net/10261/20713
Access Level:acceso abierto
Palabra clave:Photosystem II
Oxygen Evolving Complex
PsbP
Crystal structures
Spinacia oleracea
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spelling Crystallization and preliminary crystallographic characterization of the extrinsic PsbP protein of photosystem II from Spinacia oleraceaKohoutova, JaroslavaKuta-Smatanova, IvanaBrynda, JiriLapkouski, MikalaiRevuelta Doval, José LuisArellano, Juan B.Ettrich, RüdigerPhotosystem IIOxygen Evolving ComplexPsbPCrystal structuresSpinacia oleraceaPreliminary X-ray diffraction analysis of the extrinsic PsbP protein of photosystem II from spinach (Spinacia oleracea) was performed using N-terminally His-tagged recombinant PsbP protein overexpressed in Escherichia coli. Recombinant PsbP protein (thrombin-digested recombinant His-tagged PsbP) stored in bis-Tris buffer pH 6.00 was crystallized using the sitting-drop vapour diffusion technique with PEG 550 MME as a precipitant and zinc sulfate as an additive. SDS–PAGE analysis of a dissolved crystal showed that the crystals did not contain the degradation products of recombinant PsbP protein. PsbP crystals diffracted to 2.06 A resolution in space group P2(1)2(1)2(1), with unit-cell parameters a = 38.68, b = 46.73, c = 88.9 A.Support from the Institutional Research Concept of the Academy of Science of the Czech Republic (AVOZ60870520, AVOZ50520514 and AVOZ0550506) and from the Ministry of Education of the Czech Republic (LC 06010 and MSM6007665808) and the Grant Agency of the Czech Republic (203/08/0114) are gratefully acknowledged. This work was also funded by grants BFU2007-68107-C02-02/BMC (to JBA) and AGL2005-07245-C03-03 (to JLR) from the Ministerio de Education y Ciencia, Spain. We would also like to thank Ms Esther Fernandez for technical assistance.Peer reviewedBlackwell Publishing201020102009info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501525178 bytesapplication/pdfhttp://hdl.handle.net/10261/20713reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://journals.iucr.org/f/issues/2009/02/00/issconts.htmlinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/207132026-05-22T06:33:51Z
dc.title.none.fl_str_mv Crystallization and preliminary crystallographic characterization of the extrinsic PsbP protein of photosystem II from Spinacia oleracea
title Crystallization and preliminary crystallographic characterization of the extrinsic PsbP protein of photosystem II from Spinacia oleracea
spellingShingle Crystallization and preliminary crystallographic characterization of the extrinsic PsbP protein of photosystem II from Spinacia oleracea
Kohoutova, Jaroslava
Photosystem II
Oxygen Evolving Complex
PsbP
Crystal structures
Spinacia oleracea
title_short Crystallization and preliminary crystallographic characterization of the extrinsic PsbP protein of photosystem II from Spinacia oleracea
title_full Crystallization and preliminary crystallographic characterization of the extrinsic PsbP protein of photosystem II from Spinacia oleracea
title_fullStr Crystallization and preliminary crystallographic characterization of the extrinsic PsbP protein of photosystem II from Spinacia oleracea
title_full_unstemmed Crystallization and preliminary crystallographic characterization of the extrinsic PsbP protein of photosystem II from Spinacia oleracea
title_sort Crystallization and preliminary crystallographic characterization of the extrinsic PsbP protein of photosystem II from Spinacia oleracea
dc.creator.none.fl_str_mv Kohoutova, Jaroslava
Kuta-Smatanova, Ivana
Brynda, Jiri
Lapkouski, Mikalai
Revuelta Doval, José Luis
Arellano, Juan B.
Ettrich, Rüdiger
author Kohoutova, Jaroslava
author_facet Kohoutova, Jaroslava
Kuta-Smatanova, Ivana
Brynda, Jiri
Lapkouski, Mikalai
Revuelta Doval, José Luis
Arellano, Juan B.
Ettrich, Rüdiger
author_role author
author2 Kuta-Smatanova, Ivana
Brynda, Jiri
Lapkouski, Mikalai
Revuelta Doval, José Luis
Arellano, Juan B.
Ettrich, Rüdiger
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Photosystem II
Oxygen Evolving Complex
PsbP
Crystal structures
Spinacia oleracea
topic Photosystem II
Oxygen Evolving Complex
PsbP
Crystal structures
Spinacia oleracea
description Preliminary X-ray diffraction analysis of the extrinsic PsbP protein of photosystem II from spinach (Spinacia oleracea) was performed using N-terminally His-tagged recombinant PsbP protein overexpressed in Escherichia coli. Recombinant PsbP protein (thrombin-digested recombinant His-tagged PsbP) stored in bis-Tris buffer pH 6.00 was crystallized using the sitting-drop vapour diffusion technique with PEG 550 MME as a precipitant and zinc sulfate as an additive. SDS–PAGE analysis of a dissolved crystal showed that the crystals did not contain the degradation products of recombinant PsbP protein. PsbP crystals diffracted to 2.06 A resolution in space group P2(1)2(1)2(1), with unit-cell parameters a = 38.68, b = 46.73, c = 88.9 A.
publishDate 2009
dc.date.none.fl_str_mv 2009
2010
2010
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/20713
url http://hdl.handle.net/10261/20713
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://journals.iucr.org/f/issues/2009/02/00/issconts.html
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 525178 bytes
application/pdf
dc.publisher.none.fl_str_mv Blackwell Publishing
publisher.none.fl_str_mv Blackwell Publishing
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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