Strain-Specific Probiotic Properties of Bifidobacteria and Lactobacilli for the Prevention of Diarrhea Caused by Rotavirus in a Preclinical Model

Probiotic supplementationwithdifferent lactobacilli andbifidobacterial strainshasdemonstrated beneficial effects in infectious diarrhea caused by rotavirus (RV) in young children. Preclinical models of RV infection might be a good strategy to screen for the e cacy of new probiotic strains or to test...

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Detalles Bibliográficos
Autores: Azagra Boronat, Ignasi, Massot Cladera, Malen, Knipping, K., Garssen, J., Ben Amor, Kaouther, Knol, J., Franch i Masferrer, Àngels, Castell, Margarida, Rodríguez Lagunas, María José, Pérez-Cano, Francisco J.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/162197
Acceso en línea:https://hdl.handle.net/2445/162197
Access Level:acceso abierto
Palabra clave:Probiòtics
Lactobacils
Diarrea
Probiotics
Lactobacillus
Diarrhea
Descripción
Sumario:Probiotic supplementationwithdifferent lactobacilli andbifidobacterial strainshasdemonstrated beneficial effects in infectious diarrhea caused by rotavirus (RV) in young children. Preclinical models of RV infection might be a good strategy to screen for the e cacy of new probiotic strains or to test their comparative e cacy. Neonatal Lewis rats were supplemented with Bifidobacterium breve M-16V, Lactobacillus acidophilus NCFM, Lactobacillus helveticus R0052, or Lactobacillus salivarius PS2 from days 2-14 of life. On day five, animals received RV SA-11 orally. Fecal samples were collected daily, weighed, and scored for the calculation of severity and incidence of diarrhea. In addition, fecal pH and fecal viral shedding were measured. Animals were sacrificed at the end of the study and their blood was obtained for the quantification of RV-specific immunoglobulins. RV infection was induced in ~90% of the animals. All probiotics caused a reduction of several clinical variables of severity and incidence of diarrhea, except L. salivarius PS2. L. acidophilus NCFM, B. breve M-16V, and L. helveticus R0052 seemed to be very e ective probiotic strains. In addition, all Lactobacillus strains reduced the viral elimination one day post-inoculation. No di erences were detected in the specific anti-RV humoral response. The present study highlights the strain-specific e ects of probiotics and identifies promising probiotics for use in ameliorating and preventing RV-induced diarrhea in children, for example by including them in infant formulas