Biotic interactions in soil and rhizosphere: predation of Sinorhizobium meliloti by Myxococcus xanthus

Proceedings of the I Congress PIIISA celebrado en la Estación Experimental del Zaidín, Granada, el 26 de abril de 2012.

Detalles Bibliográficos
Autores: Tarifa Álvarez, Gabriela, Villar del Paso, Ana, Pérez González, Clara, Yelamos Lorente, Ángeles, Marín Sánchez, Irene, Ceballos Miján, Nazaret, Peña López, Raúl, Jurado de Haro, Manuel, Muñoz Dorado, José, Pérez, Juana, Jiménez-Zurdo, José I.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2012
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/100163
Acceso en línea:http://hdl.handle.net/10261/100163
Access Level:acceso abierto
Palabra clave:PIIISA
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spelling Biotic interactions in soil and rhizosphere: predation of Sinorhizobium meliloti by Myxococcus xanthusTarifa Álvarez, GabrielaVillar del Paso, AnaPérez González, ClaraYelamos Lorente, ÁngelesMarín Sánchez, IreneCeballos Miján, NazaretPeña López, RaúlJurado de Haro, ManuelMuñoz Dorado, JoséPérez, JuanaJiménez-Zurdo, José I.PIIISAProceedings of the I Congress PIIISA celebrado en la Estación Experimental del Zaidín, Granada, el 26 de abril de 2012.The nitrogen‐fixing legume endosymbiont Sinorhizobium meliloti and the myxobacterium Myxococcus xanthus are both Gram‐negative bacteria which could potentially interact in soil. The objectives of this research work were to assess predation patterns of S. meliloti by M. xanthus and to set up an experimental system to study this process. We first characterized the growth phenotypes of several S. meliloti strains and a reference M. xanthus strain in different culture media. We observed that the best medium for S. meliloti was the rich TY medium, where bacteria exhibited markedly exopolysaccharide (EPS)‐ dependent mucous and non‐mucous phenotypes. In contrast, M. xanthus grew better in CTT medium where it showed a stable typical swarm phenotype. Co‐inoculation assays on CTT plates showed two patterns of S. meliloti responses to predation by myxobacteria, likely dependent on EPS production. We next tagged S. meliloti strains by conjugation with a plasmid‐borne kanamycin (Km) resistance gene. Predation assays in CTT broth revealed disappearance of S. meliloti tagged bacteria in the presence of M. xanthus as inferred from time‐course plate counts in Km‐supplemented TY medium. Our results provide a base‐line to investigate the molecular bases of bacterial predation in the future.It was mainly supported by research project MICINN Consolider‐Ingenio 2010 CSD2009‐00006.Peer reviewedCSIC - Estación Experimental del Zaidín (EEZ)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201420142012info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/100163reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésHigh School Students for Agricultural Science Research. Proceedings of the I Congress PIIISA. https://digital.csic.es/handle/10261/86940Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1001632026-05-22T06:33:51Z
dc.title.none.fl_str_mv Biotic interactions in soil and rhizosphere: predation of Sinorhizobium meliloti by Myxococcus xanthus
title Biotic interactions in soil and rhizosphere: predation of Sinorhizobium meliloti by Myxococcus xanthus
spellingShingle Biotic interactions in soil and rhizosphere: predation of Sinorhizobium meliloti by Myxococcus xanthus
Tarifa Álvarez, Gabriela
PIIISA
title_short Biotic interactions in soil and rhizosphere: predation of Sinorhizobium meliloti by Myxococcus xanthus
title_full Biotic interactions in soil and rhizosphere: predation of Sinorhizobium meliloti by Myxococcus xanthus
title_fullStr Biotic interactions in soil and rhizosphere: predation of Sinorhizobium meliloti by Myxococcus xanthus
title_full_unstemmed Biotic interactions in soil and rhizosphere: predation of Sinorhizobium meliloti by Myxococcus xanthus
title_sort Biotic interactions in soil and rhizosphere: predation of Sinorhizobium meliloti by Myxococcus xanthus
dc.creator.none.fl_str_mv Tarifa Álvarez, Gabriela
Villar del Paso, Ana
Pérez González, Clara
Yelamos Lorente, Ángeles
Marín Sánchez, Irene
Ceballos Miján, Nazaret
Peña López, Raúl
Jurado de Haro, Manuel
Muñoz Dorado, José
Pérez, Juana
Jiménez-Zurdo, José I.
author Tarifa Álvarez, Gabriela
author_facet Tarifa Álvarez, Gabriela
Villar del Paso, Ana
Pérez González, Clara
Yelamos Lorente, Ángeles
Marín Sánchez, Irene
Ceballos Miján, Nazaret
Peña López, Raúl
Jurado de Haro, Manuel
Muñoz Dorado, José
Pérez, Juana
Jiménez-Zurdo, José I.
author_role author
author2 Villar del Paso, Ana
Pérez González, Clara
Yelamos Lorente, Ángeles
Marín Sánchez, Irene
Ceballos Miján, Nazaret
Peña López, Raúl
Jurado de Haro, Manuel
Muñoz Dorado, José
Pérez, Juana
Jiménez-Zurdo, José I.
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv PIIISA
topic PIIISA
description Proceedings of the I Congress PIIISA celebrado en la Estación Experimental del Zaidín, Granada, el 26 de abril de 2012.
publishDate 2012
dc.date.none.fl_str_mv 2012
2014
2014
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/100163
url http://hdl.handle.net/10261/100163
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv High School Students for Agricultural Science Research. Proceedings of the I Congress PIIISA. https://digital.csic.es/handle/10261/86940
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv CSIC - Estación Experimental del Zaidín (EEZ)
publisher.none.fl_str_mv CSIC - Estación Experimental del Zaidín (EEZ)
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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