Immunisation efficacy of a stabilised SARS-CoV-2 spike glycoprotein in two geriatric animal models

AgeisassociatedwithreducedefficacyofvaccinesandlinkedtohigherriskofsevereCOVID-19.Herewe determined the impact of ageing on the efficacy of a SARS-CoV-2 vaccine based on a stabilised Spike glycoprotein (S-29) that had previously shown high efficacy in young animals. Thirteen to 18-month-old golden S...

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Detalles Bibliográficos
Autores: Usai, Carla, Ainsua-Enrich, Erola, Urrea Gales, Victor, Pradenas, Edwards, Lorca-Oró, Cristina, Tarrés Freixas, Ferran, Roca, Núria, Pérez Maíllo, Mónica, Ávila-Nieto, Carlos, Rodríguez de la Concepción, María Luisa, Pedreño-Lopez, Núria, Carabelli, Julieta, Trinité, Benjamin, Ballana, Ester, Riveira-Muñoz, Eva, Izquierdo-Useros, Nuria, Clotet, Bonaventura, Blanco, Julià, Guallar, Victor, Cantero Portillo, Guillermo, Serra Gironella, Joan, Carrillo, Jorge, Segalés, Joaquim
Tipo de recurso: artículo
Fecha de publicación:2024
País:España
Institución:Institut de Recerca i Tecnologia Agroalimentàries (IRTA)
Repositorio:IRTA Pubpro. Open Digital Archive
OAI Identifier:oai:repositori.irta.cat:20.500.12327/2904
Acceso en línea:http://hdl.handle.net/20.500.12327/2904
https://doi.org/10.1038/s41541-024-00840-0
Access Level:acceso abierto
Palabra clave:619
Descripción
Sumario:AgeisassociatedwithreducedefficacyofvaccinesandlinkedtohigherriskofsevereCOVID-19.Herewe determined the impact of ageing on the efficacy of a SARS-CoV-2 vaccine based on a stabilised Spike glycoprotein (S-29) that had previously shown high efficacy in young animals. Thirteen to 18-month-old golden Syrian hamsters (GSH) and 22–23-month-old K18-hCAE2 mice were immunised twice with S-29 protein in AddaVaxTM adjuvant. GSH were intranasally inoculated with SARS-CoV-2 either two weeks or four months after the booster dose, while all K18-hACE2 mice were intranasally inoculated two weeks after the second immunisation. Body weight and clinical signs were recorded daily post-inoculation. Lesions and viral load were investigated in different target tissues. Immunisation induced seroconversion and production of neutralising antibodies; however, animals were only partially protected from weight loss. We observed a significant reduction in the amount of viral RNA and a faster viral protein clearance in the tissues of immunized animals. Infectious particles showed a faster decay in vaccinated animals while tissue lesion development was not altered. In GSH, the shortest interval between immunisation and inoculation reduced RNA levels in the lungs, while the longest interval was equally effective in reducing RNAinnasalturbinates; viral nucleoprotein amount decreased in both tissues. Inmice, immunisation was able to improve the survival of infected animals.Despite the high protection shown in young animals, S-29 efficacy was reduced in the geriatric population.Our research high lights the importance of testing vaccine efficacy in older animals as part of preclinical vaccine evaluation.

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