Optical modulation of neurotransmission using calcium photocurrents through the ion channel LiGluR

A wide range of light-activated molecules (photoswitches and phototriggers) have been used to the study of computational properties of an isolated neuron by acting pre and postsynaptically. However, new tools are being pursued to elicit a presynaptic calcium influx that triggers the release of neuro...

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Detalhes bibliográficos
Autores: Izquierdo Serra, Mercè, Trauner, Dirk, Llobet Berenguer, Artur, 1972-, Gorostiza Langa, Pablo Ignacio
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2013
País:España
Recursos:Universidad de Barcelona
Repositório:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/126087
Acesso em linha:https://hdl.handle.net/2445/126087
Access Level:Acceso aberto
Palavra-chave:Calci
Neurotransmissió
Òptica
Sinapsi
Calcium
Neural transmission
Optics
Synapses
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spelling Optical modulation of neurotransmission using calcium photocurrents through the ion channel LiGluRIzquierdo Serra, MercèTrauner, DirkLlobet Berenguer, Artur, 1972-Gorostiza Langa, Pablo IgnacioCalciNeurotransmissióÒpticaSinapsiCalciumNeural transmissionOpticsSynapsesA wide range of light-activated molecules (photoswitches and phototriggers) have been used to the study of computational properties of an isolated neuron by acting pre and postsynaptically. However, new tools are being pursued to elicit a presynaptic calcium influx that triggers the release of neurotransmitters, most of them based in calcium-permeable Channelrhodopsin-2 mutants. Here we describe a method to control exocytosis of synaptic vesicles through the use of a light-gated glutamate receptor (LiGluR), which has recently been demonstrated that supports secretion by means of calcium influx in chromaffin cells. Expression of LiGluR in hippocampal neurons enables reversible control of neurotransmission with light, and allows modulating the firing rate of the postsynaptic neuron with the wavelength of illumination. This method may be useful for the determination of the complex transfer function of individual synapses.Frontiers Media2013info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/126087Articles publicats en revistes (Patologia i Terapèutica Experimental)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.3389/fnmol.2013.00003Frontiers In Molecular Neuroscience, 2013, vol. 6, num. 3https://doi.org/10.3389/fnmol.2013.00003info:eu-repo/grantAgreement/EC/FP7/210355cc-by (c) Izquierdo Serra, Mercè et al., 2013http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1260872026-05-27T06:46:51Z
dc.title.none.fl_str_mv Optical modulation of neurotransmission using calcium photocurrents through the ion channel LiGluR
title Optical modulation of neurotransmission using calcium photocurrents through the ion channel LiGluR
spellingShingle Optical modulation of neurotransmission using calcium photocurrents through the ion channel LiGluR
Izquierdo Serra, Mercè
Calci
Neurotransmissió
Òptica
Sinapsi
Calcium
Neural transmission
Optics
Synapses
title_short Optical modulation of neurotransmission using calcium photocurrents through the ion channel LiGluR
title_full Optical modulation of neurotransmission using calcium photocurrents through the ion channel LiGluR
title_fullStr Optical modulation of neurotransmission using calcium photocurrents through the ion channel LiGluR
title_full_unstemmed Optical modulation of neurotransmission using calcium photocurrents through the ion channel LiGluR
title_sort Optical modulation of neurotransmission using calcium photocurrents through the ion channel LiGluR
dc.creator.none.fl_str_mv Izquierdo Serra, Mercè
Trauner, Dirk
Llobet Berenguer, Artur, 1972-
Gorostiza Langa, Pablo Ignacio
author Izquierdo Serra, Mercè
author_facet Izquierdo Serra, Mercè
Trauner, Dirk
Llobet Berenguer, Artur, 1972-
Gorostiza Langa, Pablo Ignacio
author_role author
author2 Trauner, Dirk
Llobet Berenguer, Artur, 1972-
Gorostiza Langa, Pablo Ignacio
author2_role author
author
author
dc.subject.none.fl_str_mv Calci
Neurotransmissió
Òptica
Sinapsi
Calcium
Neural transmission
Optics
Synapses
topic Calci
Neurotransmissió
Òptica
Sinapsi
Calcium
Neural transmission
Optics
Synapses
description A wide range of light-activated molecules (photoswitches and phototriggers) have been used to the study of computational properties of an isolated neuron by acting pre and postsynaptically. However, new tools are being pursued to elicit a presynaptic calcium influx that triggers the release of neurotransmitters, most of them based in calcium-permeable Channelrhodopsin-2 mutants. Here we describe a method to control exocytosis of synaptic vesicles through the use of a light-gated glutamate receptor (LiGluR), which has recently been demonstrated that supports secretion by means of calcium influx in chromaffin cells. Expression of LiGluR in hippocampal neurons enables reversible control of neurotransmission with light, and allows modulating the firing rate of the postsynaptic neuron with the wavelength of illumination. This method may be useful for the determination of the complex transfer function of individual synapses.
publishDate 2013
dc.date.none.fl_str_mv 2013
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/126087
url https://hdl.handle.net/2445/126087
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3389/fnmol.2013.00003
Frontiers In Molecular Neuroscience, 2013, vol. 6, num. 3
https://doi.org/10.3389/fnmol.2013.00003
info:eu-repo/grantAgreement/EC/FP7/210355
dc.rights.none.fl_str_mv cc-by (c) Izquierdo Serra, Mercè et al., 2013
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Izquierdo Serra, Mercè et al., 2013
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv Articles publicats en revistes (Patologia i Terapèutica Experimental)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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