Analysis of sperm capacitation and sperm selection bythermotaxis in stallion, bull and rabbit
Assisted reproductive technologies (ARTs) such as in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) are widely used in livestock production. In some species like the horse, IVF is not successful and ICSI is used whereas in others like cattle it happens oppositely. However, thes...
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| Tipo de recurso: | tesis doctoral |
| Fecha de publicación: | 2024 |
| País: | España |
| Institución: | Universidad Complutense de Madrid (UCM) |
| Repositorio: | Docta Complutense |
| Idioma: | inglés |
| OAI Identifier: | oai:docta.ucm.es:20.500.14352/108888 |
| Acceso en línea: | https://hdl.handle.net/20.500.14352/108888 |
| Access Level: | acceso abierto |
| Palabra clave: | 636.082.4(043.2) Reproducción Reproduction Veterinaria 3109 Ciencias Veterinarias |
| Sumario: | Assisted reproductive technologies (ARTs) such as in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) are widely used in livestock production. In some species like the horse, IVF is not successful and ICSI is used whereas in others like cattle it happens oppositely. However, these techniques still yield suboptimal success rates. In the horse, the blastocyst success rate using ICSI with in vitro matured oocytes can be upto 40%. In cattle, the IVF success rate in terms of cleavage can be up to 80% but embryoproduction remains between 30% to 40%. A strategy to improve ARTs is to mimic in vitro the in vivo fertilization process. The spermatozoa guidance through the female genital tract, which includes chemotaxis, rheotaxis and thermotaxis, has been proposed to be a physiological phenomenon for sperm selection. Sperm thermotaxis is defined as the ability of the spermatozoa to navigate in a temperature gradient from the lower to the higher, and it is present in rabbits, humans, mice and bulls making it to be considered a guidance mechanism conserved among species... |
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