Selective Inhibition of Clade A Phosphatases Type 2C by PYR/PYL/RCAR Abscisic Acid Receptors

[EN] Clade A protein phosphatases type 2C (PP2Cs) are negative regulators of abscisic acid (ABA) signaling that are inhibited in an ABA-dependent manner by PYRABACTIN RESISTANCE1 (PYR1)/PYR1-LIKE (PYL)/REGULATORY COMPONENTS OF ABA RECEPTORS (RCAR) intracellular receptors. We provide genetic evidence...

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Autores: Antoni-Alandes, Regina, González Guzmán, Miguel, Rodriguez, Lesia, Rodrigues, Americo, Pizzio Bianchi, Gaston Alfredo, Rodríguez Egea, Pedro Luís|||0000-0002-5886-9425
Tipo de recurso: artículo
Fecha de publicación:2012
País:España
Institución:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:riunet.upv.es:10251/82489
Acceso en línea:https://riunet.upv.es/handle/10251/82489
Access Level:acceso abierto
Palabra clave:ANION CHANNEL SLAC1
PROTEIN-KINASES
ARABIDOPSIS-THALIANA
SIGNAL-TRANSDUCTION
NEGATIVE REGULATOR
STRESS RESPONSES
MESSENGER-RNA
GUARD-CELLS
ABA
DROUGHT
MICROBIOLOGIA
BIOQUIMICA Y BIOLOGIA MOLECULAR
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network_acronym_str ES
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repository_id_str
spelling Selective Inhibition of Clade A Phosphatases Type 2C by PYR/PYL/RCAR Abscisic Acid ReceptorsAntoni-Alandes, ReginaGonzález Guzmán, MiguelRodriguez, LesiaRodrigues, AmericoPizzio Bianchi, Gaston AlfredoRodríguez Egea, Pedro Luís|||0000-0002-5886-9425ANION CHANNEL SLAC1PROTEIN-KINASESARABIDOPSIS-THALIANASIGNAL-TRANSDUCTIONNEGATIVE REGULATORSTRESS RESPONSESMESSENGER-RNAGUARD-CELLSABADROUGHTMICROBIOLOGIABIOQUIMICA Y BIOLOGIA MOLECULAR[EN] Clade A protein phosphatases type 2C (PP2Cs) are negative regulators of abscisic acid (ABA) signaling that are inhibited in an ABA-dependent manner by PYRABACTIN RESISTANCE1 (PYR1)/PYR1-LIKE (PYL)/REGULATORY COMPONENTS OF ABA RECEPTORS (RCAR) intracellular receptors. We provide genetic evidence that a previously uncharacterized member of this PP2C family in Arabidopsis (Arabidopsis thaliana), At5g59220, is a negative regulator of osmotic stress and ABA signaling and that this function was only apparent when double loss-of-function mutants with pp2ca-1/ahg3 were generated. At5g59220-green fluorescent protein and its close relative PP2CA-green fluorescent protein showed a predominant nuclear localization; however, hemagglutinin-tagged versions were also localized to cytosol and microsomal pellets. At5g59220 was selectively inhibited by some PYR/PYL ABA receptors, and close relatives of this PP2C, such as PP2CA/ABA-HYPERSENSITIVE GERMINATION3 (AHG3) and AHG1, showed a contrasting sensitivity to PYR/PYL inhibition. Interestingly, AHG1 was resistant to inhibition by the PYR/PYL receptors tested, which suggests that this seed-specific phosphatase is still able to regulate ABA signaling in the presence of ABA and PYR/PYL receptors and therefore to control the highly active ABA signaling pathway that operates during seed development. Moreover, the differential sensitivity of the phosphatases At5g59220 and PP2CA to inhibition by ABA receptors reveals a functional specialization of PYR/PYL ABA receptors to preferentially inhibit certain PP2Cs.This work was supported by the Ministerio de Ciencia e Innovacion, Fondo Europeo de Desarrollo Regional, and Consejo Superior de Investigaciones Cientificas (grant nos. BIO2008-00221 and BIO2011-23446 to P. L. R; fellowships to R.A. and L.R.; Juan de la Cierva contract to M.G.-G.).American Society of Plant BiologistsInstituto Universitario Mixto de Biología Molecular y Celular de PlantasRepositorio Institucional de la Universitat Politècnica de València Riunet20122012-02-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfapplication/pdfhttps://riunet.upv.es/handle/10251/82489reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengMinisterio de Ciencia e Innovación http://dx.doi.org/10.13039/501100004837 BIO2008-00221 REGULACION POR ACIDO ABSCISICO DE LA RESPUESTA AL ESTRES HIDRICO, CRECIMIENTO Y DESARROLLO VEGETALMinisterio de Ciencia e Innovación http://dx.doi.org/10.13039/501100004837 BIO2011-23446 SEÑALIZACION DE ABA MEDIADA POR LOS RECEPTORES PYR%2FPYL Y SU CONEXION CON LOS MECANISMOS DE RESISTENCIA A SEQUIAopen accesshttp://purl.org/coar/access_right/c_abf2Reserva de todos los derechoshttp://rightsstatements.org/vocab/InC/1.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/824892026-06-13T07:49:27Z
dc.title.none.fl_str_mv Selective Inhibition of Clade A Phosphatases Type 2C by PYR/PYL/RCAR Abscisic Acid Receptors
title Selective Inhibition of Clade A Phosphatases Type 2C by PYR/PYL/RCAR Abscisic Acid Receptors
spellingShingle Selective Inhibition of Clade A Phosphatases Type 2C by PYR/PYL/RCAR Abscisic Acid Receptors
Antoni-Alandes, Regina
ANION CHANNEL SLAC1
PROTEIN-KINASES
ARABIDOPSIS-THALIANA
SIGNAL-TRANSDUCTION
NEGATIVE REGULATOR
STRESS RESPONSES
MESSENGER-RNA
GUARD-CELLS
ABA
DROUGHT
MICROBIOLOGIA
BIOQUIMICA Y BIOLOGIA MOLECULAR
title_short Selective Inhibition of Clade A Phosphatases Type 2C by PYR/PYL/RCAR Abscisic Acid Receptors
title_full Selective Inhibition of Clade A Phosphatases Type 2C by PYR/PYL/RCAR Abscisic Acid Receptors
title_fullStr Selective Inhibition of Clade A Phosphatases Type 2C by PYR/PYL/RCAR Abscisic Acid Receptors
title_full_unstemmed Selective Inhibition of Clade A Phosphatases Type 2C by PYR/PYL/RCAR Abscisic Acid Receptors
title_sort Selective Inhibition of Clade A Phosphatases Type 2C by PYR/PYL/RCAR Abscisic Acid Receptors
dc.creator.none.fl_str_mv Antoni-Alandes, Regina
González Guzmán, Miguel
Rodriguez, Lesia
Rodrigues, Americo
Pizzio Bianchi, Gaston Alfredo
Rodríguez Egea, Pedro Luís|||0000-0002-5886-9425
author Antoni-Alandes, Regina
author_facet Antoni-Alandes, Regina
González Guzmán, Miguel
Rodriguez, Lesia
Rodrigues, Americo
Pizzio Bianchi, Gaston Alfredo
Rodríguez Egea, Pedro Luís|||0000-0002-5886-9425
author_role author
author2 González Guzmán, Miguel
Rodriguez, Lesia
Rodrigues, Americo
Pizzio Bianchi, Gaston Alfredo
Rodríguez Egea, Pedro Luís|||0000-0002-5886-9425
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto Universitario Mixto de Biología Molecular y Celular de Plantas
Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv ANION CHANNEL SLAC1
PROTEIN-KINASES
ARABIDOPSIS-THALIANA
SIGNAL-TRANSDUCTION
NEGATIVE REGULATOR
STRESS RESPONSES
MESSENGER-RNA
GUARD-CELLS
ABA
DROUGHT
MICROBIOLOGIA
BIOQUIMICA Y BIOLOGIA MOLECULAR
topic ANION CHANNEL SLAC1
PROTEIN-KINASES
ARABIDOPSIS-THALIANA
SIGNAL-TRANSDUCTION
NEGATIVE REGULATOR
STRESS RESPONSES
MESSENGER-RNA
GUARD-CELLS
ABA
DROUGHT
MICROBIOLOGIA
BIOQUIMICA Y BIOLOGIA MOLECULAR
description [EN] Clade A protein phosphatases type 2C (PP2Cs) are negative regulators of abscisic acid (ABA) signaling that are inhibited in an ABA-dependent manner by PYRABACTIN RESISTANCE1 (PYR1)/PYR1-LIKE (PYL)/REGULATORY COMPONENTS OF ABA RECEPTORS (RCAR) intracellular receptors. We provide genetic evidence that a previously uncharacterized member of this PP2C family in Arabidopsis (Arabidopsis thaliana), At5g59220, is a negative regulator of osmotic stress and ABA signaling and that this function was only apparent when double loss-of-function mutants with pp2ca-1/ahg3 were generated. At5g59220-green fluorescent protein and its close relative PP2CA-green fluorescent protein showed a predominant nuclear localization; however, hemagglutinin-tagged versions were also localized to cytosol and microsomal pellets. At5g59220 was selectively inhibited by some PYR/PYL ABA receptors, and close relatives of this PP2C, such as PP2CA/ABA-HYPERSENSITIVE GERMINATION3 (AHG3) and AHG1, showed a contrasting sensitivity to PYR/PYL inhibition. Interestingly, AHG1 was resistant to inhibition by the PYR/PYL receptors tested, which suggests that this seed-specific phosphatase is still able to regulate ABA signaling in the presence of ABA and PYR/PYL receptors and therefore to control the highly active ABA signaling pathway that operates during seed development. Moreover, the differential sensitivity of the phosphatases At5g59220 and PP2CA to inhibition by ABA receptors reveals a functional specialization of PYR/PYL ABA receptors to preferentially inhibit certain PP2Cs.
publishDate 2012
dc.date.none.fl_str_mv 2012
2012-02-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://riunet.upv.es/handle/10251/82489
url https://riunet.upv.es/handle/10251/82489
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Ciencia e Innovación http://dx.doi.org/10.13039/501100004837 BIO2008-00221 REGULACION POR ACIDO ABSCISICO DE LA RESPUESTA AL ESTRES HIDRICO, CRECIMIENTO Y DESARROLLO VEGETAL
Ministerio de Ciencia e Innovación http://dx.doi.org/10.13039/501100004837 BIO2011-23446 SEÑALIZACION DE ABA MEDIADA POR LOS RECEPTORES PYR%2FPYL Y SU CONEXION CON LOS MECANISMOS DE RESISTENCIA A SEQUIA
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reserva de todos los derechos
http://rightsstatements.org/vocab/InC/1.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reserva de todos los derechos
http://rightsstatements.org/vocab/InC/1.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society of Plant Biologists
publisher.none.fl_str_mv American Society of Plant Biologists
dc.source.none.fl_str_mv reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname:Universitat Politècnica de València (UPV)
instname_str Universitat Politècnica de València (UPV)
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869425070998290432
score 15.300719

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