Stable isotope analysis of dietary arginine accrual and disposal efficiency in male rats fed diets with different protein content.

The administration of diets with different protein/energy ratios induce variable but distinctive responses in rats; an excessive protein content tends to decrease fat accumulation, but reversion of this ratio tends to increase adipose tissue mass. The fate of N derived from amino acid metabolism is...

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Detalles Bibliográficos
Autores: Rotondo, Floriana, Sanz, Tania, Fernández López, José Antonio, Alemany, Marià, 1946-, Remesar Betlloch, Xavier
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2016
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/101362
Acceso en línea:https://hdl.handle.net/2445/101362
Access Level:acceso abierto
Palabra clave:Isòtops
Proteïnes
Alimentació animal
Isotopes
Proteins
Animal feeding
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spelling Stable isotope analysis of dietary arginine accrual and disposal efficiency in male rats fed diets with different protein content.Rotondo, FlorianaSanz, TaniaFernández López, José AntonioAlemany, Marià, 1946-Remesar Betlloch, XavierIsòtopsProteïnesAlimentació animalIsotopesProteinsAnimal feedingThe administration of diets with different protein/energy ratios induce variable but distinctive responses in rats; an excessive protein content tends to decrease fat accumulation, but reversion of this ratio tends to increase adipose tissue mass. The fate of N derived from amino acid metabolism is not only dependent on energy and dietary protein; the increased excretion of urea elicited by high-protein diets contrasts with the lower urea excretion (despite excess dietary protein and energy) in rats fed a cafeteria diet. After one month of exposure to high-protein (HPD) or cafeteria (CD) diets, we administered a gavage of 15N-arginine to undisturbed adult male rats, in order to trace the utilization of this not-recyclable-N amino acid under diets with different protein/energy relationships. Rats fed high-protein diet excreted higher amounts of N in urine and showed much lower gastrointestinal content of label. The CD rats decreased the excretion of urine N. Both groups' N balance showed a significant proportion of N not-accounted-for (but excreted nevertheless), the proportion being especially large in the HPD group. In conclusion, the process of disposal of amino acid N through the so far unknown pathway for 'non-accounted-for N' is, thus essentially dependent on excess amino acid availability; independently of urea cycle operation and diet energy content.Royal Society of Chemistry2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttps://hdl.handle.net/2445/101362Articles publicats en revistes (Bioquímica i Biomedicina Molecular)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésVersió postprint del document publicat a: http://dx.doi.org/10.1039/C6RA11039HRSC Advances, 2016, vol. 6, p. 69177-69184http://dx.doi.org/10.1039/C6RA11039H(c) Rotondo, Floriana et al., 2016info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1013622026-05-27T06:46:51Z
dc.title.none.fl_str_mv Stable isotope analysis of dietary arginine accrual and disposal efficiency in male rats fed diets with different protein content.
title Stable isotope analysis of dietary arginine accrual and disposal efficiency in male rats fed diets with different protein content.
spellingShingle Stable isotope analysis of dietary arginine accrual and disposal efficiency in male rats fed diets with different protein content.
Rotondo, Floriana
Isòtops
Proteïnes
Alimentació animal
Isotopes
Proteins
Animal feeding
title_short Stable isotope analysis of dietary arginine accrual and disposal efficiency in male rats fed diets with different protein content.
title_full Stable isotope analysis of dietary arginine accrual and disposal efficiency in male rats fed diets with different protein content.
title_fullStr Stable isotope analysis of dietary arginine accrual and disposal efficiency in male rats fed diets with different protein content.
title_full_unstemmed Stable isotope analysis of dietary arginine accrual and disposal efficiency in male rats fed diets with different protein content.
title_sort Stable isotope analysis of dietary arginine accrual and disposal efficiency in male rats fed diets with different protein content.
dc.creator.none.fl_str_mv Rotondo, Floriana
Sanz, Tania
Fernández López, José Antonio
Alemany, Marià, 1946-
Remesar Betlloch, Xavier
author Rotondo, Floriana
author_facet Rotondo, Floriana
Sanz, Tania
Fernández López, José Antonio
Alemany, Marià, 1946-
Remesar Betlloch, Xavier
author_role author
author2 Sanz, Tania
Fernández López, José Antonio
Alemany, Marià, 1946-
Remesar Betlloch, Xavier
author2_role author
author
author
author
dc.subject.none.fl_str_mv Isòtops
Proteïnes
Alimentació animal
Isotopes
Proteins
Animal feeding
topic Isòtops
Proteïnes
Alimentació animal
Isotopes
Proteins
Animal feeding
description The administration of diets with different protein/energy ratios induce variable but distinctive responses in rats; an excessive protein content tends to decrease fat accumulation, but reversion of this ratio tends to increase adipose tissue mass. The fate of N derived from amino acid metabolism is not only dependent on energy and dietary protein; the increased excretion of urea elicited by high-protein diets contrasts with the lower urea excretion (despite excess dietary protein and energy) in rats fed a cafeteria diet. After one month of exposure to high-protein (HPD) or cafeteria (CD) diets, we administered a gavage of 15N-arginine to undisturbed adult male rats, in order to trace the utilization of this not-recyclable-N amino acid under diets with different protein/energy relationships. Rats fed high-protein diet excreted higher amounts of N in urine and showed much lower gastrointestinal content of label. The CD rats decreased the excretion of urine N. Both groups' N balance showed a significant proportion of N not-accounted-for (but excreted nevertheless), the proportion being especially large in the HPD group. In conclusion, the process of disposal of amino acid N through the so far unknown pathway for 'non-accounted-for N' is, thus essentially dependent on excess amino acid availability; independently of urea cycle operation and diet energy content.
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/101362
url https://hdl.handle.net/2445/101362
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: http://dx.doi.org/10.1039/C6RA11039H
RSC Advances, 2016, vol. 6, p. 69177-69184
http://dx.doi.org/10.1039/C6RA11039H
dc.rights.none.fl_str_mv (c) Rotondo, Floriana et al., 2016
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Rotondo, Floriana et al., 2016
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv Articles publicats en revistes (Bioquímica i Biomedicina Molecular)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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