Effects of Inhaled Corticosteroids on the Innate Immunological Response to Pseudomonas aeruginosa Infection in Patients with COPD

Inhaled corticosteroids (ICS) use is associated with an increased risk of Pseudomonas aeruginosa (PA) infection in patients with COPD. We aimed to evaluate the effects of ICS on alveolar macrophages in response to PA in COPD patients with and without baseline ICS treatment (COPD and COPD + ICS, resp...

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Autores: Cerón-Pisa, Noemi, Shafiek, Hanaa, Martín-Medina, Aina, Verdú, Javier, Jordana-Lluch, Elena, Escobar-Salom, Maria, Bover Barceló, Isabel, López-Causapé, Carla, Oliver, Antonio, Juan, Carlos, Iglesias, Amanda, García-Cosío, Borja
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Conselleria de Salut i Consum del Govern de les Illes Balears
Repositorio:Docusalut
Idioma:inglés
OAI Identifier:oai:docusalut.com:20.500.13003/18198
Acceso en línea:https://hdl.handle.net/20.500.13003/18198
Access Level:acceso abierto
Palabra clave:Budesonide
Administration, Inhalation
Adrenal Cortex Hormones
Toll-Like Receptor 2
Pulmonary Disease, Chronic Obstructive
Humans
Pseudomonas Infections
Enfermedad Pulmonar Obstructiva Crónica
Receptor Toll-Like 2
Humanos
Budesonida
Infecciones por Pseudomonas
Corticoesteroides
Administración por Inhalación
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spelling Effects of Inhaled Corticosteroids on the Innate Immunological Response to Pseudomonas aeruginosa Infection in Patients with COPDCerón-Pisa, NoemiShafiek, HanaaMartín-Medina, AinaVerdú, JavierJordana-Lluch, ElenaEscobar-Salom, MariaBover Barceló, IsabelLópez-Causapé, CarlaOliver, AntonioJuan, CarlosIglesias, AmandaGarcía-Cosío, BorjaBudesonideAdministration, InhalationAdrenal Cortex HormonesToll-Like Receptor 2Pulmonary Disease, Chronic ObstructiveHumansPseudomonas InfectionsEnfermedad Pulmonar Obstructiva CrónicaReceptor Toll-Like 2HumanosBudesonidaInfecciones por PseudomonasCorticoesteroidesAdministración por InhalaciónInhaled corticosteroids (ICS) use is associated with an increased risk of Pseudomonas aeruginosa (PA) infection in patients with COPD. We aimed to evaluate the effects of ICS on alveolar macrophages in response to PA in COPD patients with and without baseline ICS treatment (COPD and COPD + ICS, respectively) as well as smoker and nonsmoker controls. To do so, cells were infected with PA and cotreated with budesonide (BUD) or fluticasone propionate (FLU). The analysis of NF-κB and c-jun activity revealed a significant increase in both factors in response to PA cotreated with BUD/FLU in smokers but not in COPD or COPD + ICS patients when compared with PA infection alone. The expression of Toll-like receptor 2 (TLR2) and the transcription factor c-jun were induced upon PA infection in nonsmokers only. Moreover, in the smoker and COPD groups, there was a significant increase in TLR2 and a decrease in c-jun expression when treated with BUD/FLU after PA infection, which were not observed in COPD + ICS patients. Therefore, the chronic use of ICS seemingly makes the macrophages tolerant to BUD/FLU stimulation compared with those from patients not treated with ICS, promoting an impaired recognition of PA and activity of alveolar macrophages in terms of altered expression of TLR2 and cytokine production, which could explain the increased risk of PA infection in COPD patients under ICS treatment.20222022-07-2320222022-07-23research articlehttp://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.13003/18198reponame:Docusalutinstname:Conselleria de Salut i Consum del Govern de les Illes BalearsInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:docusalut.com:20.500.13003/181982026-06-22T12:44:07Z
dc.title.none.fl_str_mv Effects of Inhaled Corticosteroids on the Innate Immunological Response to Pseudomonas aeruginosa Infection in Patients with COPD
title Effects of Inhaled Corticosteroids on the Innate Immunological Response to Pseudomonas aeruginosa Infection in Patients with COPD
spellingShingle Effects of Inhaled Corticosteroids on the Innate Immunological Response to Pseudomonas aeruginosa Infection in Patients with COPD
Cerón-Pisa, Noemi
Budesonide
Administration, Inhalation
Adrenal Cortex Hormones
Toll-Like Receptor 2
Pulmonary Disease, Chronic Obstructive
Humans
Pseudomonas Infections
Enfermedad Pulmonar Obstructiva Crónica
Receptor Toll-Like 2
Humanos
Budesonida
Infecciones por Pseudomonas
Corticoesteroides
Administración por Inhalación
title_short Effects of Inhaled Corticosteroids on the Innate Immunological Response to Pseudomonas aeruginosa Infection in Patients with COPD
title_full Effects of Inhaled Corticosteroids on the Innate Immunological Response to Pseudomonas aeruginosa Infection in Patients with COPD
title_fullStr Effects of Inhaled Corticosteroids on the Innate Immunological Response to Pseudomonas aeruginosa Infection in Patients with COPD
title_full_unstemmed Effects of Inhaled Corticosteroids on the Innate Immunological Response to Pseudomonas aeruginosa Infection in Patients with COPD
title_sort Effects of Inhaled Corticosteroids on the Innate Immunological Response to Pseudomonas aeruginosa Infection in Patients with COPD
dc.creator.none.fl_str_mv Cerón-Pisa, Noemi
Shafiek, Hanaa
Martín-Medina, Aina
Verdú, Javier
Jordana-Lluch, Elena
Escobar-Salom, Maria
Bover Barceló, Isabel
López-Causapé, Carla
Oliver, Antonio
Juan, Carlos
Iglesias, Amanda
García-Cosío, Borja
author Cerón-Pisa, Noemi
author_facet Cerón-Pisa, Noemi
Shafiek, Hanaa
Martín-Medina, Aina
Verdú, Javier
Jordana-Lluch, Elena
Escobar-Salom, Maria
Bover Barceló, Isabel
López-Causapé, Carla
Oliver, Antonio
Juan, Carlos
Iglesias, Amanda
García-Cosío, Borja
author_role author
author2 Shafiek, Hanaa
Martín-Medina, Aina
Verdú, Javier
Jordana-Lluch, Elena
Escobar-Salom, Maria
Bover Barceló, Isabel
López-Causapé, Carla
Oliver, Antonio
Juan, Carlos
Iglesias, Amanda
García-Cosío, Borja
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv
dc.subject.none.fl_str_mv Budesonide
Administration, Inhalation
Adrenal Cortex Hormones
Toll-Like Receptor 2
Pulmonary Disease, Chronic Obstructive
Humans
Pseudomonas Infections
Enfermedad Pulmonar Obstructiva Crónica
Receptor Toll-Like 2
Humanos
Budesonida
Infecciones por Pseudomonas
Corticoesteroides
Administración por Inhalación
topic Budesonide
Administration, Inhalation
Adrenal Cortex Hormones
Toll-Like Receptor 2
Pulmonary Disease, Chronic Obstructive
Humans
Pseudomonas Infections
Enfermedad Pulmonar Obstructiva Crónica
Receptor Toll-Like 2
Humanos
Budesonida
Infecciones por Pseudomonas
Corticoesteroides
Administración por Inhalación
description Inhaled corticosteroids (ICS) use is associated with an increased risk of Pseudomonas aeruginosa (PA) infection in patients with COPD. We aimed to evaluate the effects of ICS on alveolar macrophages in response to PA in COPD patients with and without baseline ICS treatment (COPD and COPD + ICS, respectively) as well as smoker and nonsmoker controls. To do so, cells were infected with PA and cotreated with budesonide (BUD) or fluticasone propionate (FLU). The analysis of NF-κB and c-jun activity revealed a significant increase in both factors in response to PA cotreated with BUD/FLU in smokers but not in COPD or COPD + ICS patients when compared with PA infection alone. The expression of Toll-like receptor 2 (TLR2) and the transcription factor c-jun were induced upon PA infection in nonsmokers only. Moreover, in the smoker and COPD groups, there was a significant increase in TLR2 and a decrease in c-jun expression when treated with BUD/FLU after PA infection, which were not observed in COPD + ICS patients. Therefore, the chronic use of ICS seemingly makes the macrophages tolerant to BUD/FLU stimulation compared with those from patients not treated with ICS, promoting an impaired recognition of PA and activity of alveolar macrophages in terms of altered expression of TLR2 and cytokine production, which could explain the increased risk of PA infection in COPD patients under ICS treatment.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-07-23
2022
2022-07-23
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.13003/18198
url https://hdl.handle.net/20.500.13003/18198
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Docusalut
instname:Conselleria de Salut i Consum del Govern de les Illes Balears
instname_str Conselleria de Salut i Consum del Govern de les Illes Balears
reponame_str Docusalut
collection Docusalut
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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