Metschnikowia drakensbergensis sp. nov. and Metschnikowia caudata sp. nov., two endemic yeasts associated with Protea flowers in South Africa

In a taxonomic study of yeasts recovered from nectar of flowers and associated insects in South Africa, eleven strains were found to represent two novel species. Morphological and physiological characteristics and sequence analyses of the D1/D2 large subunit rRNA gene, as well as the actin, RNA poly...

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Autores: Vega, Clara de, Guzmán, Beatriz, Steenhuisen, Sandy-Lynn, Johnson, Steven D., Herrera, Carlos M., Lachance, Marc-André
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2014
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/102810
Acceso en línea:http://hdl.handle.net/10261/102810
Access Level:acceso abierto
Palabra clave:Biodiversity
Flower-beetle-yeast
Metschnikowia
New yeast species
Protea
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spelling Metschnikowia drakensbergensis sp. nov. and Metschnikowia caudata sp. nov., two endemic yeasts associated with Protea flowers in South AfricaVega, Clara deGuzmán, BeatrizSteenhuisen, Sandy-LynnJohnson, Steven D.Herrera, Carlos M.Lachance, Marc-AndréBiodiversityFlower-beetle-yeastMetschnikowiaNew yeast speciesProteaIn a taxonomic study of yeasts recovered from nectar of flowers and associated insects in South Africa, eleven strains were found to represent two novel species. Morphological and physiological characteristics and sequence analyses of the D1/D2 large subunit rRNA gene, as well as the actin, RNA polymerase II, and elongation factor 2 genes showed that the two novel species belonged to the genus Metschnikowia. Metschnikowia drakensbergensis sp. nov. was recovered from nectar of Protea roupelliae and the beetle Heterochelus sp. This species belongs to the large-spored Metschnikowia clade and is closely related to M. proteae, with which mating reactions and single-spored asci were observed. Metschnikowia caudata sp. nov. was isolated from nectar of P. dracomontana, P. roupelliae, P. subvestita and a honey bee, and is a sister species to Candida hainanensis and M. lopburiensis. Analyses of the four genes demonstrated the existence of three separate phylotypes. Intraspecies matings lead to the production of mature asci of unprecedented morphology, with a long, flexuous tail. A single ascospore was produced in all compatible crosses, regardless of sequence phylotype. The two species appear to be endemic to South Africa. The ecology and habitat specificity of these novel species is discussed in terms of host plant and insect host species. The type cultures are: Metschnikowia drakensbergensis (type strain EBD-CdVSA09-2T=CBS 13649T=NRRL Y-63721T, MycoBank no. MB809688; allotype EBD-CdVSA10-2A=CBS13650A=NRRL Y-63720A); and Metschnikowia caudata (type strain EBD-CdVSA08-1T=CBS 13651T=NRRL Y-63722T, MycoBank no. MB809689; allotype EBD-CdVSA57-2A=CBS 13729A=NRRL Y-63723A)Estación Biológica de Doñana/Unidad de Excelencia Severo Ochoa. Financiado: Ministerio de Economía y Competitividad (España)Peer reviewedSociety for General MicrobiologyConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201420142014info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/102810reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1099/ijs.0.068445-0Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1028102026-05-22T06:33:51Z
dc.title.none.fl_str_mv Metschnikowia drakensbergensis sp. nov. and Metschnikowia caudata sp. nov., two endemic yeasts associated with Protea flowers in South Africa
title Metschnikowia drakensbergensis sp. nov. and Metschnikowia caudata sp. nov., two endemic yeasts associated with Protea flowers in South Africa
spellingShingle Metschnikowia drakensbergensis sp. nov. and Metschnikowia caudata sp. nov., two endemic yeasts associated with Protea flowers in South Africa
Vega, Clara de
Biodiversity
Flower-beetle-yeast
Metschnikowia
New yeast species
Protea
title_short Metschnikowia drakensbergensis sp. nov. and Metschnikowia caudata sp. nov., two endemic yeasts associated with Protea flowers in South Africa
title_full Metschnikowia drakensbergensis sp. nov. and Metschnikowia caudata sp. nov., two endemic yeasts associated with Protea flowers in South Africa
title_fullStr Metschnikowia drakensbergensis sp. nov. and Metschnikowia caudata sp. nov., two endemic yeasts associated with Protea flowers in South Africa
title_full_unstemmed Metschnikowia drakensbergensis sp. nov. and Metschnikowia caudata sp. nov., two endemic yeasts associated with Protea flowers in South Africa
title_sort Metschnikowia drakensbergensis sp. nov. and Metschnikowia caudata sp. nov., two endemic yeasts associated with Protea flowers in South Africa
dc.creator.none.fl_str_mv Vega, Clara de
Guzmán, Beatriz
Steenhuisen, Sandy-Lynn
Johnson, Steven D.
Herrera, Carlos M.
Lachance, Marc-André
author Vega, Clara de
author_facet Vega, Clara de
Guzmán, Beatriz
Steenhuisen, Sandy-Lynn
Johnson, Steven D.
Herrera, Carlos M.
Lachance, Marc-André
author_role author
author2 Guzmán, Beatriz
Steenhuisen, Sandy-Lynn
Johnson, Steven D.
Herrera, Carlos M.
Lachance, Marc-André
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Biodiversity
Flower-beetle-yeast
Metschnikowia
New yeast species
Protea
topic Biodiversity
Flower-beetle-yeast
Metschnikowia
New yeast species
Protea
description In a taxonomic study of yeasts recovered from nectar of flowers and associated insects in South Africa, eleven strains were found to represent two novel species. Morphological and physiological characteristics and sequence analyses of the D1/D2 large subunit rRNA gene, as well as the actin, RNA polymerase II, and elongation factor 2 genes showed that the two novel species belonged to the genus Metschnikowia. Metschnikowia drakensbergensis sp. nov. was recovered from nectar of Protea roupelliae and the beetle Heterochelus sp. This species belongs to the large-spored Metschnikowia clade and is closely related to M. proteae, with which mating reactions and single-spored asci were observed. Metschnikowia caudata sp. nov. was isolated from nectar of P. dracomontana, P. roupelliae, P. subvestita and a honey bee, and is a sister species to Candida hainanensis and M. lopburiensis. Analyses of the four genes demonstrated the existence of three separate phylotypes. Intraspecies matings lead to the production of mature asci of unprecedented morphology, with a long, flexuous tail. A single ascospore was produced in all compatible crosses, regardless of sequence phylotype. The two species appear to be endemic to South Africa. The ecology and habitat specificity of these novel species is discussed in terms of host plant and insect host species. The type cultures are: Metschnikowia drakensbergensis (type strain EBD-CdVSA09-2T=CBS 13649T=NRRL Y-63721T, MycoBank no. MB809688; allotype EBD-CdVSA10-2A=CBS13650A=NRRL Y-63720A); and Metschnikowia caudata (type strain EBD-CdVSA08-1T=CBS 13651T=NRRL Y-63722T, MycoBank no. MB809689; allotype EBD-CdVSA57-2A=CBS 13729A=NRRL Y-63723A)
publishDate 2014
dc.date.none.fl_str_mv 2014
2014
2014
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/102810
url http://hdl.handle.net/10261/102810
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1099/ijs.0.068445-0
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Society for General Microbiology
publisher.none.fl_str_mv Society for General Microbiology
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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