Astrocyte reactivity after brain injury-: The role of galectins 1 and 3

Astrocytes react to brain injury in a heterogeneous manner with only a subset resuming proliferation and acquiring stem cell properties in vitro. In order to identify novel regulators of this subset, we performed genomewide expression analysis of reactive astrocytes isolated 5 days after stab wound...

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Autores: Sirko, Swetlana, Irmler, Martin, Gascón, Sergio, Bek, Sarah, Schneider, Sarah, Dimou, Leda, Obermann, Jara, Souza Paiva, Daisylea de, Poirier, Francoise, Beckers, Johannes, Hauck, Stefanie M, Barde, Yves Alain, Götz, Magdalena
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/346248
Acceso en línea:http://hdl.handle.net/10261/346248
https://api.elsevier.com/content/abstract/scopus_id/84943580834
Access Level:acceso abierto
Palabra clave:genomewide analysis
glia proliferation
neurosphere
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spelling Astrocyte reactivity after brain injury-: The role of galectins 1 and 3Sirko, SwetlanaIrmler, MartinGascón, SergioBek, SarahSchneider, SarahDimou, LedaObermann, JaraSouza Paiva, Daisylea dePoirier, FrancoiseBeckers, JohannesHauck, Stefanie MBarde, Yves AlainGötz, Magdalenagenomewide analysisglia proliferationneurosphereAstrocytes react to brain injury in a heterogeneous manner with only a subset resuming proliferation and acquiring stem cell properties in vitro. In order to identify novel regulators of this subset, we performed genomewide expression analysis of reactive astrocytes isolated 5 days after stab wound injury from the gray matter of adult mouse cerebral cortex. The expression pattern was compared with astrocytes from intact cortex and adult neural stem cells (NSCs) isolated from the subependymal zone (SEZ). These comparisons revealed a set of genes expressed at higher levels in both endogenous NSCs and reactive astrocytes, including two lectins-Galectins 1 and 3. These results and the pattern of Galectin expression in the lesioned brain led us to examine the functional significance of these lectins in brains of mice lacking Galectins 1 and 3. Following stab wound injury, astrocyte reactivity including glial fibrillary acidic protein expression, proliferation and neurosphere-forming capacity were found significantly reduced in mutant animals. This phenotype could be recapitulated in vitro and was fully rescued by addition of Galectin 3, but not of Galectin 1. Thus, Galectins 1 and 3 play key roles in regulating the proliferative and NSC potential of a subset of reactive astrocytes.Grant sponsor: DFG; Grant numbers: SPP 1757 Functional heterogeneity of glia (MG); SFB 871 neural Circuits (MG);HA 6014/2-2 (SMH); Grant sponsor: Synergy Excellence Cluster, the Helmholtz Foundation (ICEMED Alliance) (MG); Grant sponsor: ERC; Grant number: 340793 (MG).The authors thank Gabi Jager, Carmen Meyer, Andrea Stein er-Mezzadri, Tatiana Simon-Ebert and Detlef Franzenfor excellent technical assistance, Dr. Corinna Haupt for greathelp in the initial phase of the Galectin mutant project and Dr. Ruth Beckervordersandforth for great help in sorting ofreactive astrocytes.Peer reviewedJohn Wiley & SonsHelmholtz AssociationConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242015info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/346248https://api.elsevier.com/content/abstract/scopus_id/84943580834reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésGliahttps://onlinelibrary.wiley.com/doi/epdf/10.1002/glia.22898Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3462482026-05-22T06:33:51Z
dc.title.none.fl_str_mv Astrocyte reactivity after brain injury-: The role of galectins 1 and 3
title Astrocyte reactivity after brain injury-: The role of galectins 1 and 3
spellingShingle Astrocyte reactivity after brain injury-: The role of galectins 1 and 3
Sirko, Swetlana
genomewide analysis
glia proliferation
neurosphere
title_short Astrocyte reactivity after brain injury-: The role of galectins 1 and 3
title_full Astrocyte reactivity after brain injury-: The role of galectins 1 and 3
title_fullStr Astrocyte reactivity after brain injury-: The role of galectins 1 and 3
title_full_unstemmed Astrocyte reactivity after brain injury-: The role of galectins 1 and 3
title_sort Astrocyte reactivity after brain injury-: The role of galectins 1 and 3
dc.creator.none.fl_str_mv Sirko, Swetlana
Irmler, Martin
Gascón, Sergio
Bek, Sarah
Schneider, Sarah
Dimou, Leda
Obermann, Jara
Souza Paiva, Daisylea de
Poirier, Francoise
Beckers, Johannes
Hauck, Stefanie M
Barde, Yves Alain
Götz, Magdalena
author Sirko, Swetlana
author_facet Sirko, Swetlana
Irmler, Martin
Gascón, Sergio
Bek, Sarah
Schneider, Sarah
Dimou, Leda
Obermann, Jara
Souza Paiva, Daisylea de
Poirier, Francoise
Beckers, Johannes
Hauck, Stefanie M
Barde, Yves Alain
Götz, Magdalena
author_role author
author2 Irmler, Martin
Gascón, Sergio
Bek, Sarah
Schneider, Sarah
Dimou, Leda
Obermann, Jara
Souza Paiva, Daisylea de
Poirier, Francoise
Beckers, Johannes
Hauck, Stefanie M
Barde, Yves Alain
Götz, Magdalena
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Helmholtz Association
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv genomewide analysis
glia proliferation
neurosphere
topic genomewide analysis
glia proliferation
neurosphere
description Astrocytes react to brain injury in a heterogeneous manner with only a subset resuming proliferation and acquiring stem cell properties in vitro. In order to identify novel regulators of this subset, we performed genomewide expression analysis of reactive astrocytes isolated 5 days after stab wound injury from the gray matter of adult mouse cerebral cortex. The expression pattern was compared with astrocytes from intact cortex and adult neural stem cells (NSCs) isolated from the subependymal zone (SEZ). These comparisons revealed a set of genes expressed at higher levels in both endogenous NSCs and reactive astrocytes, including two lectins-Galectins 1 and 3. These results and the pattern of Galectin expression in the lesioned brain led us to examine the functional significance of these lectins in brains of mice lacking Galectins 1 and 3. Following stab wound injury, astrocyte reactivity including glial fibrillary acidic protein expression, proliferation and neurosphere-forming capacity were found significantly reduced in mutant animals. This phenotype could be recapitulated in vitro and was fully rescued by addition of Galectin 3, but not of Galectin 1. Thus, Galectins 1 and 3 play key roles in regulating the proliferative and NSC potential of a subset of reactive astrocytes.
publishDate 2015
dc.date.none.fl_str_mv 2015
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/346248
https://api.elsevier.com/content/abstract/scopus_id/84943580834
url http://hdl.handle.net/10261/346248
https://api.elsevier.com/content/abstract/scopus_id/84943580834
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Glia
https://onlinelibrary.wiley.com/doi/epdf/10.1002/glia.22898
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv John Wiley & Sons
publisher.none.fl_str_mv John Wiley & Sons
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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