Preferential enhancement of sensory and motor axon regeneration by combining extracellular matrix components with neurotrophic factors

After peripheral nerve injury, motor and sensory axons are able to regenerate but inaccuracy of target reinnervation leads to poor functional recovery. Extracellular matrix (ECM) components and neurotrophic factors (NTFs) exert their effect on different neuronal populations creating a suitable envir...

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Autores: Santos, Daniel, González Pérez, Francisco J., Giudetti, Guido|||0000-0002-4543-5871, Micera, Silvestro|||0000-0003-4396-8217, Udina i Bonet, Esther|||0000-0003-1954-8562, Del Valle, Jaume|||0000-0002-6703-8244, Navarro, X. (Xavier)|||0000-0001-9849-902X
Tipo de recurso: artículo
Fecha de publicación:2017
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:169699
Acceso en línea:https://ddd.uab.cat/record/169699
https://dx.doi.org/urn:doi:10.3390/ijms18010065
Access Level:acceso abierto
Palabra clave:Neurotrophic factors
BDNF
NGF
NT3
Extracellular matrix
Motor axons
Sensory axons
Nerve regeneration
Reinnervation
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spelling Preferential enhancement of sensory and motor axon regeneration by combining extracellular matrix components with neurotrophic factorsSantos, DanielGonzález Pérez, Francisco J.Giudetti, Guido|||0000-0002-4543-5871Micera, Silvestro|||0000-0003-4396-8217Udina i Bonet, Esther|||0000-0003-1954-8562Del Valle, Jaume|||0000-0002-6703-8244Navarro, X. (Xavier)|||0000-0001-9849-902XNeurotrophic factorsBDNFNGFNT3Extracellular matrixMotor axonsSensory axonsNerve regenerationReinnervationAfter peripheral nerve injury, motor and sensory axons are able to regenerate but inaccuracy of target reinnervation leads to poor functional recovery. Extracellular matrix (ECM) components and neurotrophic factors (NTFs) exert their effect on different neuronal populations creating a suitable environment to promote axonal growth. Here, we assessed in vitro and in vivo the selective effects of combining different ECM components with NTFs on motor and sensory axons regeneration and target reinnervation. Organotypic cultures with collagen, laminin and nerve growth factor (NGF)/neurotrophin-3 (NT3) or collagen, fibronectin and brain-derived neurotrophic factor (BDNF) selectively enhanced sensory neurite outgrowth of DRG neurons and motor neurite outgrowth from spinal cord slices respectively. For in vivo studies, the rat sciatic nerve was transected and repaired with a silicone tube filled with a collagen and laminin matrix with NGF/NT3 encapsulated in poly(lactic-co-glycolic acid) (PLGA) microspheres (MP) (LM + MP.NGF/NT3), or a collagen and fibronectin matrix with BDNF in PLGA MPs (FN + MP.BDNF). Retrograde labeling and functional tests showed that LM + MP.NGF/NT3 increased the number of regenerated sensory neurons and improved sensory functional recovery, whereas FN + MP.BDNF preferentially increased regenerated motoneurons and enhanced motor functional recovery. Therefore, combination of ECM molecules with NTFs may be a good approach to selectively enhance motor and sensory axons regeneration and promote appropriate target reinnervation 22017-01-0120172017-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/169699https://dx.doi.org/urn:doi:10.3390/ijms18010065reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengEuropean Commission https://doi.org/10.13039/501100000780 280778European Commission https://doi.org/10.13039/501100000780 611687open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:1696992026-06-06T12:50:31Z
dc.title.none.fl_str_mv Preferential enhancement of sensory and motor axon regeneration by combining extracellular matrix components with neurotrophic factors
title Preferential enhancement of sensory and motor axon regeneration by combining extracellular matrix components with neurotrophic factors
spellingShingle Preferential enhancement of sensory and motor axon regeneration by combining extracellular matrix components with neurotrophic factors
Santos, Daniel
Neurotrophic factors
BDNF
NGF
NT3
Extracellular matrix
Motor axons
Sensory axons
Nerve regeneration
Reinnervation
title_short Preferential enhancement of sensory and motor axon regeneration by combining extracellular matrix components with neurotrophic factors
title_full Preferential enhancement of sensory and motor axon regeneration by combining extracellular matrix components with neurotrophic factors
title_fullStr Preferential enhancement of sensory and motor axon regeneration by combining extracellular matrix components with neurotrophic factors
title_full_unstemmed Preferential enhancement of sensory and motor axon regeneration by combining extracellular matrix components with neurotrophic factors
title_sort Preferential enhancement of sensory and motor axon regeneration by combining extracellular matrix components with neurotrophic factors
dc.creator.none.fl_str_mv Santos, Daniel
González Pérez, Francisco J.
Giudetti, Guido|||0000-0002-4543-5871
Micera, Silvestro|||0000-0003-4396-8217
Udina i Bonet, Esther|||0000-0003-1954-8562
Del Valle, Jaume|||0000-0002-6703-8244
Navarro, X. (Xavier)|||0000-0001-9849-902X
author Santos, Daniel
author_facet Santos, Daniel
González Pérez, Francisco J.
Giudetti, Guido|||0000-0002-4543-5871
Micera, Silvestro|||0000-0003-4396-8217
Udina i Bonet, Esther|||0000-0003-1954-8562
Del Valle, Jaume|||0000-0002-6703-8244
Navarro, X. (Xavier)|||0000-0001-9849-902X
author_role author
author2 González Pérez, Francisco J.
Giudetti, Guido|||0000-0002-4543-5871
Micera, Silvestro|||0000-0003-4396-8217
Udina i Bonet, Esther|||0000-0003-1954-8562
Del Valle, Jaume|||0000-0002-6703-8244
Navarro, X. (Xavier)|||0000-0001-9849-902X
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Neurotrophic factors
BDNF
NGF
NT3
Extracellular matrix
Motor axons
Sensory axons
Nerve regeneration
Reinnervation
topic Neurotrophic factors
BDNF
NGF
NT3
Extracellular matrix
Motor axons
Sensory axons
Nerve regeneration
Reinnervation
description After peripheral nerve injury, motor and sensory axons are able to regenerate but inaccuracy of target reinnervation leads to poor functional recovery. Extracellular matrix (ECM) components and neurotrophic factors (NTFs) exert their effect on different neuronal populations creating a suitable environment to promote axonal growth. Here, we assessed in vitro and in vivo the selective effects of combining different ECM components with NTFs on motor and sensory axons regeneration and target reinnervation. Organotypic cultures with collagen, laminin and nerve growth factor (NGF)/neurotrophin-3 (NT3) or collagen, fibronectin and brain-derived neurotrophic factor (BDNF) selectively enhanced sensory neurite outgrowth of DRG neurons and motor neurite outgrowth from spinal cord slices respectively. For in vivo studies, the rat sciatic nerve was transected and repaired with a silicone tube filled with a collagen and laminin matrix with NGF/NT3 encapsulated in poly(lactic-co-glycolic acid) (PLGA) microspheres (MP) (LM + MP.NGF/NT3), or a collagen and fibronectin matrix with BDNF in PLGA MPs (FN + MP.BDNF). Retrograde labeling and functional tests showed that LM + MP.NGF/NT3 increased the number of regenerated sensory neurons and improved sensory functional recovery, whereas FN + MP.BDNF preferentially increased regenerated motoneurons and enhanced motor functional recovery. Therefore, combination of ECM molecules with NTFs may be a good approach to selectively enhance motor and sensory axons regeneration and promote appropriate target reinnervation
publishDate 2017
dc.date.none.fl_str_mv 2
2017-01-01
2017
2017-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/169699
https://dx.doi.org/urn:doi:10.3390/ijms18010065
url https://ddd.uab.cat/record/169699
https://dx.doi.org/urn:doi:10.3390/ijms18010065
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv European Commission https://doi.org/10.13039/501100000780 280778
European Commission https://doi.org/10.13039/501100000780 611687
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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