Immunomodulatory Effects and Regulatory Mechanisms of (R)-6-HITC, an Isothiocyanate from Wasabi (Eutrema japonicum), in an Ex Vivo Mouse Model of LPS-Induced Inflammation
The present study aimed to investigate the effects of (R)-(−)-1-isothiocyanato-6-(methylsulfinyl)-hexane [(R)-6-HITC], the major isothiocyanate present in wasabi, in an ex vivo model of inflammation using lipopolysaccharide-stimulated murine peritoneal macrophages. (R)-6-HITC improved the immune res...
| Autores: | , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2024 |
| País: | España |
| Institución: | Universidad de Sevilla (US) |
| Repositorio: | idUS. Depósito de Investigación de la Universidad de Sevilla |
| OAI Identifier: | oai:idus.us.es:11441/168259 |
| Acceso en línea: | https://hdl.handle.net/11441/168259 https://doi.org/10.1021/acs.jafc.4c02943 |
| Access Level: | acceso abierto |
| Palabra clave: | (R)-6-HITC DAG-methodology Eutrema japonicum Inflammation Murine peritoneal macrophages Wasabi |
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Immunomodulatory Effects and Regulatory Mechanisms of (R)-6-HITC, an Isothiocyanate from Wasabi (Eutrema japonicum), in an Ex Vivo Mouse Model of LPS-Induced InflammationAlcarranza Saucedo, ManuelAlarcón de la Lastra Romero, CatalinaRecio Jiménez, RocíoFernández Fernández, InmaculadaCastejón Martínez, María LuisaVillegas Lama, Isabel(R)-6-HITCDAG-methodologyEutrema japonicumInflammationMurine peritoneal macrophagesWasabiThe present study aimed to investigate the effects of (R)-(−)-1-isothiocyanato-6-(methylsulfinyl)-hexane [(R)-6-HITC], the major isothiocyanate present in wasabi, in an ex vivo model of inflammation using lipopolysaccharide-stimulated murine peritoneal macrophages. (R)-6-HITC improved the immune response and mitigated oxidative stress, which involved suppression of reactive oxygen species, nitric oxide, and pro-inflammatory cytokines (IL-1β, IL-6, IL-17, IL-18, and TNF-α) production and downregulation of pro-inflammatory enzymes such as inducible nitric oxide synthase, COX-2, and mPGES-1. In addition, (R)-6-HITC was able to activate the Nrf2/HO-1 axis while simultaneously inhibiting key signaling pathways, including JAK2/STAT3, mitogen-activated protein kinases, and canonical and noncanonical inflammasome pathways, orchestrating its potent immunomodulatory effects. Collectively, these findings demonstrate the potential of (R)-6-HITC as a promising nutraceutical for the management of immuno-inflammatory diseases and justify the need for further in vivo validation studies.Ministerio de Ciencia, Innovación y Universidades PDC2022−133,627-I00, PID2019−104767RB-I00Junta de Andalucía FQM-102, 2021/CTS-259, CTS-259Premio Anual Publicación Científica Destacada de la US. Facultad de FarmaciaAmerican Chemical SocietyFarmacologíaQuímica Orgánica y FarmacéuticaMinisterio de Ciencia, Innovación y Universidades (MICINN). EspañaJunta de Andalucía2024info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/168259https://doi.org/10.1021/acs.jafc.4c02943reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésJournal of Agricultural and Food Chemistry, 72 (39), 21520-21532.PDC2022−133,627-I00PID2019−104767RB-I00FQM-1022021/CTS-259CTS-259https://doi.org/10.1021/acs.jafc.4c02943info:eu-repo/semantics/openAccessoai:idus.us.es:11441/1682592026-06-17T12:51:07Z |
| dc.title.none.fl_str_mv |
Immunomodulatory Effects and Regulatory Mechanisms of (R)-6-HITC, an Isothiocyanate from Wasabi (Eutrema japonicum), in an Ex Vivo Mouse Model of LPS-Induced Inflammation |
| title |
Immunomodulatory Effects and Regulatory Mechanisms of (R)-6-HITC, an Isothiocyanate from Wasabi (Eutrema japonicum), in an Ex Vivo Mouse Model of LPS-Induced Inflammation |
| spellingShingle |
Immunomodulatory Effects and Regulatory Mechanisms of (R)-6-HITC, an Isothiocyanate from Wasabi (Eutrema japonicum), in an Ex Vivo Mouse Model of LPS-Induced Inflammation Alcarranza Saucedo, Manuel (R)-6-HITC DAG-methodology Eutrema japonicum Inflammation Murine peritoneal macrophages Wasabi |
| title_short |
Immunomodulatory Effects and Regulatory Mechanisms of (R)-6-HITC, an Isothiocyanate from Wasabi (Eutrema japonicum), in an Ex Vivo Mouse Model of LPS-Induced Inflammation |
| title_full |
Immunomodulatory Effects and Regulatory Mechanisms of (R)-6-HITC, an Isothiocyanate from Wasabi (Eutrema japonicum), in an Ex Vivo Mouse Model of LPS-Induced Inflammation |
| title_fullStr |
Immunomodulatory Effects and Regulatory Mechanisms of (R)-6-HITC, an Isothiocyanate from Wasabi (Eutrema japonicum), in an Ex Vivo Mouse Model of LPS-Induced Inflammation |
| title_full_unstemmed |
Immunomodulatory Effects and Regulatory Mechanisms of (R)-6-HITC, an Isothiocyanate from Wasabi (Eutrema japonicum), in an Ex Vivo Mouse Model of LPS-Induced Inflammation |
| title_sort |
Immunomodulatory Effects and Regulatory Mechanisms of (R)-6-HITC, an Isothiocyanate from Wasabi (Eutrema japonicum), in an Ex Vivo Mouse Model of LPS-Induced Inflammation |
| dc.creator.none.fl_str_mv |
Alcarranza Saucedo, Manuel Alarcón de la Lastra Romero, Catalina Recio Jiménez, Rocío Fernández Fernández, Inmaculada Castejón Martínez, María Luisa Villegas Lama, Isabel |
| author |
Alcarranza Saucedo, Manuel |
| author_facet |
Alcarranza Saucedo, Manuel Alarcón de la Lastra Romero, Catalina Recio Jiménez, Rocío Fernández Fernández, Inmaculada Castejón Martínez, María Luisa Villegas Lama, Isabel |
| author_role |
author |
| author2 |
Alarcón de la Lastra Romero, Catalina Recio Jiménez, Rocío Fernández Fernández, Inmaculada Castejón Martínez, María Luisa Villegas Lama, Isabel |
| author2_role |
author author author author author |
| dc.contributor.none.fl_str_mv |
Farmacología Química Orgánica y Farmacéutica Ministerio de Ciencia, Innovación y Universidades (MICINN). España Junta de Andalucía |
| dc.subject.none.fl_str_mv |
(R)-6-HITC DAG-methodology Eutrema japonicum Inflammation Murine peritoneal macrophages Wasabi |
| topic |
(R)-6-HITC DAG-methodology Eutrema japonicum Inflammation Murine peritoneal macrophages Wasabi |
| description |
The present study aimed to investigate the effects of (R)-(−)-1-isothiocyanato-6-(methylsulfinyl)-hexane [(R)-6-HITC], the major isothiocyanate present in wasabi, in an ex vivo model of inflammation using lipopolysaccharide-stimulated murine peritoneal macrophages. (R)-6-HITC improved the immune response and mitigated oxidative stress, which involved suppression of reactive oxygen species, nitric oxide, and pro-inflammatory cytokines (IL-1β, IL-6, IL-17, IL-18, and TNF-α) production and downregulation of pro-inflammatory enzymes such as inducible nitric oxide synthase, COX-2, and mPGES-1. In addition, (R)-6-HITC was able to activate the Nrf2/HO-1 axis while simultaneously inhibiting key signaling pathways, including JAK2/STAT3, mitogen-activated protein kinases, and canonical and noncanonical inflammasome pathways, orchestrating its potent immunomodulatory effects. Collectively, these findings demonstrate the potential of (R)-6-HITC as a promising nutraceutical for the management of immuno-inflammatory diseases and justify the need for further in vivo validation studies. |
| publishDate |
2024 |
| dc.date.none.fl_str_mv |
2024 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/11441/168259 https://doi.org/10.1021/acs.jafc.4c02943 |
| url |
https://hdl.handle.net/11441/168259 https://doi.org/10.1021/acs.jafc.4c02943 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
Journal of Agricultural and Food Chemistry, 72 (39), 21520-21532. PDC2022−133,627-I00 PID2019−104767RB-I00 FQM-102 2021/CTS-259 CTS-259 https://doi.org/10.1021/acs.jafc.4c02943 |
| dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf application/pdf |
| dc.publisher.none.fl_str_mv |
American Chemical Society |
| publisher.none.fl_str_mv |
American Chemical Society |
| dc.source.none.fl_str_mv |
reponame:idUS. Depósito de Investigación de la Universidad de Sevilla instname:Universidad de Sevilla (US) |
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Universidad de Sevilla (US) |
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idUS. Depósito de Investigación de la Universidad de Sevilla |
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idUS. Depósito de Investigación de la Universidad de Sevilla |
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15,198674 |