Cryogels and Monoliths: Promising Tools for Chromatographic Purification of Nucleic Acids

© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

Detalles Bibliográficos
Autores: Ribeiro, João, Luís, Marco Â., Rodrigues, Bruno, Santos, Fátima M., Mesquita, Joana, Boto, Renato, Tomaz, Cândida Teixeira
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/352792
Acceso en línea:http://hdl.handle.net/10261/352792
Access Level:acceso abierto
Palabra clave:Chromatography
Cryogels
Monoliths
pDNA
Purification
RNA
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spelling Cryogels and Monoliths: Promising Tools for Chromatographic Purification of Nucleic AcidsRibeiro, JoãoLuís, Marco Â.Rodrigues, BrunoSantos, Fátima M.Mesquita, JoanaBoto, RenatoTomaz, Cândida TeixeiraChromatographyCryogelsMonolithspDNAPurificationRNA© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).The increasing demand for highly pure biopharmaceuticals has put significant pressure on the biotechnological industry to innovate in production and purification processes. Nucleic acid purification, crucial for gene therapy and vaccine production, presents challenges due to the unique physical and chemical properties of these molecules. Meeting regulatory standards necessitates large quantities of biotherapeutic agents of high purity. While conventional chromatography offers versatility and efficiency, it suffers from drawbacks like low flow rates and binding capacity, as well as high mass transfer resistance. Recent advancements in continuous beds, including monoliths and cryogel-based systems, have emerged as promising solutions to overcome these limitations. This review explores and evaluates the latest progress in chromatography utilizing monolithic and cryogenic supports for nucleic acid purification.This research was funded by CICS-UBI projects UIDB/00709/2020 and UIDP/00709/2020, financed by national funds through the Portuguese Foundation for Science and Technology/MCTES. We thank CICS-UBI, Health Sciences Research Centre, Universidade da Beira Interior, for funding the COMBINE collaborative project and Centro-01-0145-FEDER-000019-C4-Centro de Competências em Cloud Computing, co-financed by the European Regional Development Fund (ERDF) through the Programa Operacional Regional do Centro (Centro 2020), in the scope of the Sistema de Apoio à Investigação Científica e Tecnológica-Programas Integrados de IC&DT.Peer reviewedMultidisciplinary Digital Publishing InstituteFundação para a Ciência e a Tecnologia (Portugal)Universidade da Beira InteriorEuropean CommissionConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2024202420242024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/352792reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.3390/gels10030198Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3527922026-05-22T06:33:51Z
dc.title.none.fl_str_mv Cryogels and Monoliths: Promising Tools for Chromatographic Purification of Nucleic Acids
title Cryogels and Monoliths: Promising Tools for Chromatographic Purification of Nucleic Acids
spellingShingle Cryogels and Monoliths: Promising Tools for Chromatographic Purification of Nucleic Acids
Ribeiro, João
Chromatography
Cryogels
Monoliths
pDNA
Purification
RNA
title_short Cryogels and Monoliths: Promising Tools for Chromatographic Purification of Nucleic Acids
title_full Cryogels and Monoliths: Promising Tools for Chromatographic Purification of Nucleic Acids
title_fullStr Cryogels and Monoliths: Promising Tools for Chromatographic Purification of Nucleic Acids
title_full_unstemmed Cryogels and Monoliths: Promising Tools for Chromatographic Purification of Nucleic Acids
title_sort Cryogels and Monoliths: Promising Tools for Chromatographic Purification of Nucleic Acids
dc.creator.none.fl_str_mv Ribeiro, João
Luís, Marco Â.
Rodrigues, Bruno
Santos, Fátima M.
Mesquita, Joana
Boto, Renato
Tomaz, Cândida Teixeira
author Ribeiro, João
author_facet Ribeiro, João
Luís, Marco Â.
Rodrigues, Bruno
Santos, Fátima M.
Mesquita, Joana
Boto, Renato
Tomaz, Cândida Teixeira
author_role author
author2 Luís, Marco Â.
Rodrigues, Bruno
Santos, Fátima M.
Mesquita, Joana
Boto, Renato
Tomaz, Cândida Teixeira
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Fundação para a Ciência e a Tecnologia (Portugal)
Universidade da Beira Interior
European Commission
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Chromatography
Cryogels
Monoliths
pDNA
Purification
RNA
topic Chromatography
Cryogels
Monoliths
pDNA
Purification
RNA
description © 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
publishDate 2024
dc.date.none.fl_str_mv 2024
2024
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/352792
url http://hdl.handle.net/10261/352792
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.3390/gels10030198
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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