Identification and Molecular Characterization of Four New Blastocystis Subtypes Designated ST35-ST38

Three recent studies of Blastocystis epidemiology in mammalian hosts identified four novel sequences that appeared to share B. lapemi as the most similar sequence. However, full-length ssu rRNA gene sequences were not available to confirm the validity of these new subtypes. In the present study, Nan...

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Detalles Bibliográficos
Autores: Maloney, Jenny G., Molokin, Aleksey, Seguí, Raimundo, Maravilla, Pablo, Martínez Hernández, Fernando, Villalobos, Guiehdani, Tsaousis, Anastasios D., Gentekaki, Eleni, Muñoz-Antolí, Carla, Klisiowicz, Debora R., Oishi, Camila Y., Toledo, Rafael, Esteban, J. Guillermo, Köster, Pamela C., de Lucio, Aida, Dashti, Alejandro, Bailo, Begoña, Calero Bernal, Rafael, González Barrio, David, Carmena, David, Santín, Mónica
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/73199
Acceso en línea:https://hdl.handle.net/20.500.14352/73199
Access Level:acceso abierto
Palabra clave:Blastocystis
B. lapemi
Subtype
Oxford Nanopore MinION
Ssu rRNA
Microbiología (Veterinaria)
3109.05 Microbiología
Descripción
Sumario:Three recent studies of Blastocystis epidemiology in mammalian hosts identified four novel sequences that appeared to share B. lapemi as the most similar sequence. However, full-length ssu rRNA gene sequences were not available to confirm the validity of these new subtypes. In the present study, Nanopore MinION sequencing was used to obtain full-length reference sequences for each of the new subtypes. Additionally, phylogenetic analyses and pairwise distance comparisons were performed to confirm the validity of each of these new subtypes. We propose that the novel sequences described in this study should be assigned the subtype designations ST35-ST38. The full-length reference sequences of ST35-ST38 will assist in accurate sequence descriptions in future studies of Blastocystis epidemiology and subtype diversity.

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