The Domestic Environment and the Lung Mycobiome

This study analyzes the relationship between the mycobiome of the Lower Respiratory Tract (LRT) and the fungi in the domestic environment. Samples studied consisted of Broncho-Alveolar Lavage (BAL) from 45 patients who underwent bronchoscopy for different diagnostic purposes, and dust and air from t...

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Autores: Rubio-Portillo E, Orts D, Llorca E, Fernández C, Antón J, Ferrer C, Gálvez B, Esteban V, Revelles E, Pérez-Martín C, Gómez-Imbernón E, Adsuar J, Piqueras P, Amat B, Franco J, Colom MF
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
Repositorio:r-FISABIO. Repositorio Institucional de Producción Científica
OAI Identifier:oai:fisabio.fundanetsuite.com:p8219
Acceso en línea:https://fisabio.portalinvestigacion.com/publicaciones/8219
Access Level:acceso abierto
Palabra clave:fungi
mycobiome
lower respiratory tract
house dust
mycobiota
bronco-alveolar lavage
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spelling The Domestic Environment and the Lung MycobiomeRubio-Portillo EOrts DLlorca EFernández CAntón JFerrer CGálvez BEsteban VRevelles EPérez-Martín CGómez-Imbernón EAdsuar JPiqueras PAmat BFranco JColom MFfungimycobiomelower respiratory tracthouse dustmycobiotabronco-alveolar lavageThis study analyzes the relationship between the mycobiome of the Lower Respiratory Tract (LRT) and the fungi in the domestic environment. Samples studied consisted of Broncho-Alveolar Lavage (BAL) from 45 patients who underwent bronchoscopy for different diagnostic purposes, and dust and air from the houses (ENV) of 20 of them (44.4%). Additionally, five bronchoscopes (BS) were also analyzed and negative controls were included for every procedure. All samples were processed for DNA extraction and cultures, which were performed in Sabouraud Dextrose and Potato Dextrose Agar. The fungal Internal Transcribed Spacer (ITS2) was sequenced by the Solexa/Illumina system and sequences were analyzed by QIIME 1.8.0 and compared with the UNITE Database for identification. The similarity between the two fungal communities (BAL and ENV) for a specific patient was assessed via the percentage of coincidence in the detection of specific operational taxonomic units (OTUs), and about 75% of co-occurrence was detected between the mycobiome of the LRT and the houses. Cultures confirmed the presence of the core mycobiome species. However, the low rate of isolation from BAL suggests that most of its mycobiome corresponds to non-culturable cells. This likely depends on the patient's immune system activity and inflammatory status.MDPI2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://fisabio.portalinvestigacion.com/publicaciones/8219MicroorganismsISSN: 20762607reponame:r-FISABIO. Repositorio Institucional de Producción Científicainstname:Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)Inglésinfo:eu-repo/semantics/openAccessoai:fisabio.fundanetsuite.com:p82192026-06-11T12:45:17Z
dc.title.none.fl_str_mv The Domestic Environment and the Lung Mycobiome
title The Domestic Environment and the Lung Mycobiome
spellingShingle The Domestic Environment and the Lung Mycobiome
Rubio-Portillo E
fungi
mycobiome
lower respiratory tract
house dust
mycobiota
bronco-alveolar lavage
title_short The Domestic Environment and the Lung Mycobiome
title_full The Domestic Environment and the Lung Mycobiome
title_fullStr The Domestic Environment and the Lung Mycobiome
title_full_unstemmed The Domestic Environment and the Lung Mycobiome
title_sort The Domestic Environment and the Lung Mycobiome
dc.creator.none.fl_str_mv Rubio-Portillo E
Orts D
Llorca E
Fernández C
Antón J
Ferrer C
Gálvez B
Esteban V
Revelles E
Pérez-Martín C
Gómez-Imbernón E
Adsuar J
Piqueras P
Amat B
Franco J
Colom MF
author Rubio-Portillo E
author_facet Rubio-Portillo E
Orts D
Llorca E
Fernández C
Antón J
Ferrer C
Gálvez B
Esteban V
Revelles E
Pérez-Martín C
Gómez-Imbernón E
Adsuar J
Piqueras P
Amat B
Franco J
Colom MF
author_role author
author2 Orts D
Llorca E
Fernández C
Antón J
Ferrer C
Gálvez B
Esteban V
Revelles E
Pérez-Martín C
Gómez-Imbernón E
Adsuar J
Piqueras P
Amat B
Franco J
Colom MF
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv fungi
mycobiome
lower respiratory tract
house dust
mycobiota
bronco-alveolar lavage
topic fungi
mycobiome
lower respiratory tract
house dust
mycobiota
bronco-alveolar lavage
description This study analyzes the relationship between the mycobiome of the Lower Respiratory Tract (LRT) and the fungi in the domestic environment. Samples studied consisted of Broncho-Alveolar Lavage (BAL) from 45 patients who underwent bronchoscopy for different diagnostic purposes, and dust and air from the houses (ENV) of 20 of them (44.4%). Additionally, five bronchoscopes (BS) were also analyzed and negative controls were included for every procedure. All samples were processed for DNA extraction and cultures, which were performed in Sabouraud Dextrose and Potato Dextrose Agar. The fungal Internal Transcribed Spacer (ITS2) was sequenced by the Solexa/Illumina system and sequences were analyzed by QIIME 1.8.0 and compared with the UNITE Database for identification. The similarity between the two fungal communities (BAL and ENV) for a specific patient was assessed via the percentage of coincidence in the detection of specific operational taxonomic units (OTUs), and about 75% of co-occurrence was detected between the mycobiome of the LRT and the houses. Cultures confirmed the presence of the core mycobiome species. However, the low rate of isolation from BAL suggests that most of its mycobiome corresponds to non-culturable cells. This likely depends on the patient's immune system activity and inflammatory status.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://fisabio.portalinvestigacion.com/publicaciones/8219
url https://fisabio.portalinvestigacion.com/publicaciones/8219
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Microorganisms
ISSN: 20762607
reponame:r-FISABIO. Repositorio Institucional de Producción Científica
instname:Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
instname_str Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
reponame_str r-FISABIO. Repositorio Institucional de Producción Científica
collection r-FISABIO. Repositorio Institucional de Producción Científica
repository.name.fl_str_mv
repository.mail.fl_str_mv
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