Antioxidant-Based Therapy Reduces Early-Stage Intestinal Ischemia-Reperfusion Injury in Rats

Intestinal ischemia-reperfusion injury (i-IRI) is a rare disorder with a high mortality rate, resulting from the loss of blood flow to an intestinal segment. Most of the damage is triggered by the restoration of flow and the arrival of cytokines and reactive oxygen species (ROS), among others. Inact...

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Authors: Gutiérrez Sánchez, Gaizka, García-Alonso Montoya, Ignacio, Gutiérrez Sáenz de Santa María, Jorge, Alonso Varona, Ana Isabel, Herrero de la Parte, Borja
Format: article
Publication Date:2021
Country:España
Institution:Universidad del País Vasco
Repository:Addi. Archivo Digital para la Docencia y la Investigación
OAI Identifier:oai:addi.ehu.eus:10810/52027
Online Access:http://hdl.handle.net/10810/52027
Access Level:Open access
Keyword:antioxidant treatment
intestinal ischemia-reperfusion injury
allopurinol
nitroindazole
animal model
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spelling Antioxidant-Based Therapy Reduces Early-Stage Intestinal Ischemia-Reperfusion Injury in RatsGutiérrez Sánchez, GaizkaGarcía-Alonso Montoya, IgnacioGutiérrez Sáenz de Santa María, JorgeAlonso Varona, Ana IsabelHerrero de la Parte, Borjaantioxidant treatmentintestinal ischemia-reperfusion injuryallopurinolnitroindazoleanimal modelIntestinal ischemia-reperfusion injury (i-IRI) is a rare disorder with a high mortality rate, resulting from the loss of blood flow to an intestinal segment. Most of the damage is triggered by the restoration of flow and the arrival of cytokines and reactive oxygen species (ROS), among others. Inactivation of these molecules before tissue reperfusion could reduce intestinal damage. The aim of this work was to analyze the preventive effect of allopurinol and nitroindazole on intestinal mucosal damage after i-IRI. Wag/RijHsd rats were subjected to i-IRI by clamping the superior mesenteric artery (for 1 or 2 h) followed by a 30 min period of reperfusion. Histopathological intestinal damage (HID) was assessed by microscopic examination of histological sections obtained from injured intestine. HID was increased by almost 20% by doubling the ischemia time (from 1 to 2 h). Nitroindazole reduced HID in both the 1 and 2 h period of ischemia by approximately 30% and 60%, respectively (p < 0.001). Our preliminary results demonstrate that nitroindazole has a preventive/protective effect against tissue damage in the early stages of i-IRI. However, to better understand the molecular mechanisms underlying this phenomenon, further studies are needed.This research received funding from the University of The Basque Country UPV/EHU (grant reference GIU19/088).MDPI2021202120212021info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10810/52027reponame:Addi. Archivo Digital para la Docencia y la Investigacióninstname:Universidad del País VascoIngléshttps://www.mdpi.com/2076-3921/10/6/853/htminfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/3.0/es/2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).oai:addi.ehu.eus:10810/520272026-06-18T09:23:17Z
dc.title.none.fl_str_mv Antioxidant-Based Therapy Reduces Early-Stage Intestinal Ischemia-Reperfusion Injury in Rats
title Antioxidant-Based Therapy Reduces Early-Stage Intestinal Ischemia-Reperfusion Injury in Rats
spellingShingle Antioxidant-Based Therapy Reduces Early-Stage Intestinal Ischemia-Reperfusion Injury in Rats
Gutiérrez Sánchez, Gaizka
antioxidant treatment
intestinal ischemia-reperfusion injury
allopurinol
nitroindazole
animal model
title_short Antioxidant-Based Therapy Reduces Early-Stage Intestinal Ischemia-Reperfusion Injury in Rats
title_full Antioxidant-Based Therapy Reduces Early-Stage Intestinal Ischemia-Reperfusion Injury in Rats
title_fullStr Antioxidant-Based Therapy Reduces Early-Stage Intestinal Ischemia-Reperfusion Injury in Rats
title_full_unstemmed Antioxidant-Based Therapy Reduces Early-Stage Intestinal Ischemia-Reperfusion Injury in Rats
title_sort Antioxidant-Based Therapy Reduces Early-Stage Intestinal Ischemia-Reperfusion Injury in Rats
dc.creator.none.fl_str_mv Gutiérrez Sánchez, Gaizka
García-Alonso Montoya, Ignacio
Gutiérrez Sáenz de Santa María, Jorge
Alonso Varona, Ana Isabel
Herrero de la Parte, Borja
author Gutiérrez Sánchez, Gaizka
author_facet Gutiérrez Sánchez, Gaizka
García-Alonso Montoya, Ignacio
Gutiérrez Sáenz de Santa María, Jorge
Alonso Varona, Ana Isabel
Herrero de la Parte, Borja
author_role author
author2 García-Alonso Montoya, Ignacio
Gutiérrez Sáenz de Santa María, Jorge
Alonso Varona, Ana Isabel
Herrero de la Parte, Borja
author2_role author
author
author
author
dc.subject.none.fl_str_mv antioxidant treatment
intestinal ischemia-reperfusion injury
allopurinol
nitroindazole
animal model
topic antioxidant treatment
intestinal ischemia-reperfusion injury
allopurinol
nitroindazole
animal model
description Intestinal ischemia-reperfusion injury (i-IRI) is a rare disorder with a high mortality rate, resulting from the loss of blood flow to an intestinal segment. Most of the damage is triggered by the restoration of flow and the arrival of cytokines and reactive oxygen species (ROS), among others. Inactivation of these molecules before tissue reperfusion could reduce intestinal damage. The aim of this work was to analyze the preventive effect of allopurinol and nitroindazole on intestinal mucosal damage after i-IRI. Wag/RijHsd rats were subjected to i-IRI by clamping the superior mesenteric artery (for 1 or 2 h) followed by a 30 min period of reperfusion. Histopathological intestinal damage (HID) was assessed by microscopic examination of histological sections obtained from injured intestine. HID was increased by almost 20% by doubling the ischemia time (from 1 to 2 h). Nitroindazole reduced HID in both the 1 and 2 h period of ischemia by approximately 30% and 60%, respectively (p < 0.001). Our preliminary results demonstrate that nitroindazole has a preventive/protective effect against tissue damage in the early stages of i-IRI. However, to better understand the molecular mechanisms underlying this phenomenon, further studies are needed.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10810/52027
url http://hdl.handle.net/10810/52027
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://www.mdpi.com/2076-3921/10/6/853/htm
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/3.0/es/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Addi. Archivo Digital para la Docencia y la Investigación
instname:Universidad del País Vasco
instname_str Universidad del País Vasco
reponame_str Addi. Archivo Digital para la Docencia y la Investigación
collection Addi. Archivo Digital para la Docencia y la Investigación
repository.name.fl_str_mv
repository.mail.fl_str_mv
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