Do mosquitoes transmit the avian malaria-like parasite Haemoproteus? An experimental test of vector competence using mosquito saliva

[Background] The life-cycle of many vector-borne pathogens includes an asexual replication phase in the vertebrate host and sexual reproduction in the insect vector. However, as only a small array of parasites can successfully develop infective phases inside an insect, few insect species are compete...

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Autores: Gutiérrez-López, Rafael, Martínez de la Puente, Josué, Gangoso, Laura, Yan, Jiayue, Soriguer, Ramón C., Figuerola, Jordi
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/141404
Acceso en línea:http://hdl.handle.net/10261/141404
Access Level:acceso abierto
Palabra clave:Culex pipiens
Haemosporidians
Parasite transmission
Plasmodium
Vector-borne diseases
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spelling Do mosquitoes transmit the avian malaria-like parasite Haemoproteus? An experimental test of vector competence using mosquito salivaGutiérrez-López, RafaelMartínez de la Puente, JosuéGangoso, LauraYan, JiayueSoriguer, Ramón C.Figuerola, JordiCulex pipiensHaemosporidiansParasite transmissionPlasmodiumVector-borne diseases[Background] The life-cycle of many vector-borne pathogens includes an asexual replication phase in the vertebrate host and sexual reproduction in the insect vector. However, as only a small array of parasites can successfully develop infective phases inside an insect, few insect species are competent vectors for these pathogens. Molecular approaches have identified the potential insect vectors of blood parasites under natural conditions. However, the effectiveness of this methodology for verifying mosquito competence in the transmission of avian malaria parasites and related haemosporidians is still under debate. This is mainly because positive amplifications of parasite DNA in mosquitoes can be obtained not only from sporozoites, the infective phase of the malaria parasites that migrate to salivary glands, but also from different non-infective parasite forms in the body of the vector. Here, we assessed the vectorial capacity of the common mosquito Culex pipiens in the transmission of two parasite genera.[Methods] A total of 1,560 mosquitoes were allowed to feed on five house sparrows Passer domesticus naturally infected by Haemoproteus or co-infected by Haemoproteus/Plasmodium. A saliva sample of the mosquitoes that survived after 13 days post-exposure was taken to determine the presence of parasite DNA by PCR.[Results] Overall, 31.2% mosquito’s head-thorax and 5.8% saliva samples analysed showed positive amplifications for avian malaria parasites. In contrast to Haemoproteus DNA, which was not found in either the body parts or the saliva, Plasmodium DNA was detected in both the head-thorax and the saliva of mosquitoes. Parasites isolated from mosquitoes feeding on the same bird corresponded to the same Plasmodium lineage.[Conclusions] Our experiment provides good evidence for the competence of Cx. pipiens in the transmission of Plasmodium but not of Haemoproteus. Molecular analyses of saliva are an effective method for testing the vector competence of mosquitoes and other insects in the transmission of vector-borne pathogens.This study was funded by projects CGL2012-30759 and CGL2015-65055-P from the Spanish Ministerio de Economia y Competitividad and European Regional Development’s funds (FEDER). We acknowledge support of the publication fee by the CSIC Open Access Publication Support Initiative through its Unit of Information Resources for Research (URICI). R.G.L. was supported by a FPI grant (BES-2013-065274), J.M.P. by a Juan de la Cierva contract, L.G. by a contract under the Excelence Projects from Junta de Andalucía (RNM-7800) and J.Y. by a grant from China Scholarship Council.Peer reviewedBioMed CentralMinisterio de Economía y Competitividad (España)European CommissionCSIC - Unidad de Recursos de Información Científica para la Investigación (URICI)Junta de AndalucíaChina Scholarship CouncilConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201620162016info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/141404reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1186/s13071-016-1903-9Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1414042026-05-22T06:33:51Z
dc.title.none.fl_str_mv Do mosquitoes transmit the avian malaria-like parasite Haemoproteus? An experimental test of vector competence using mosquito saliva
title Do mosquitoes transmit the avian malaria-like parasite Haemoproteus? An experimental test of vector competence using mosquito saliva
spellingShingle Do mosquitoes transmit the avian malaria-like parasite Haemoproteus? An experimental test of vector competence using mosquito saliva
Gutiérrez-López, Rafael
Culex pipiens
Haemosporidians
Parasite transmission
Plasmodium
Vector-borne diseases
title_short Do mosquitoes transmit the avian malaria-like parasite Haemoproteus? An experimental test of vector competence using mosquito saliva
title_full Do mosquitoes transmit the avian malaria-like parasite Haemoproteus? An experimental test of vector competence using mosquito saliva
title_fullStr Do mosquitoes transmit the avian malaria-like parasite Haemoproteus? An experimental test of vector competence using mosquito saliva
title_full_unstemmed Do mosquitoes transmit the avian malaria-like parasite Haemoproteus? An experimental test of vector competence using mosquito saliva
title_sort Do mosquitoes transmit the avian malaria-like parasite Haemoproteus? An experimental test of vector competence using mosquito saliva
dc.creator.none.fl_str_mv Gutiérrez-López, Rafael
Martínez de la Puente, Josué
Gangoso, Laura
Yan, Jiayue
Soriguer, Ramón C.
Figuerola, Jordi
author Gutiérrez-López, Rafael
author_facet Gutiérrez-López, Rafael
Martínez de la Puente, Josué
Gangoso, Laura
Yan, Jiayue
Soriguer, Ramón C.
Figuerola, Jordi
author_role author
author2 Martínez de la Puente, Josué
Gangoso, Laura
Yan, Jiayue
Soriguer, Ramón C.
Figuerola, Jordi
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
European Commission
CSIC - Unidad de Recursos de Información Científica para la Investigación (URICI)
Junta de Andalucía
China Scholarship Council
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Culex pipiens
Haemosporidians
Parasite transmission
Plasmodium
Vector-borne diseases
topic Culex pipiens
Haemosporidians
Parasite transmission
Plasmodium
Vector-borne diseases
description [Background] The life-cycle of many vector-borne pathogens includes an asexual replication phase in the vertebrate host and sexual reproduction in the insect vector. However, as only a small array of parasites can successfully develop infective phases inside an insect, few insect species are competent vectors for these pathogens. Molecular approaches have identified the potential insect vectors of blood parasites under natural conditions. However, the effectiveness of this methodology for verifying mosquito competence in the transmission of avian malaria parasites and related haemosporidians is still under debate. This is mainly because positive amplifications of parasite DNA in mosquitoes can be obtained not only from sporozoites, the infective phase of the malaria parasites that migrate to salivary glands, but also from different non-infective parasite forms in the body of the vector. Here, we assessed the vectorial capacity of the common mosquito Culex pipiens in the transmission of two parasite genera.
publishDate 2016
dc.date.none.fl_str_mv 2016
2016
2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/141404
url http://hdl.handle.net/10261/141404
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1186/s13071-016-1903-9
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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