Real-time aptapcr: a novel approach exploiting nucleic acid aptamers for ultrasensitive detection of analytes for clinical diagnostic and in food analysis

The thesis aimed to develop and characterize a novel detection approach, which we termed aptaPCR exploiting nucleic acid aptamers as combined recognition and reporter biocomponents for the ultrasensitive detection of analytes. Nucleic acid aptamers are synthetic ligands selected from vast combinator...

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Bibliographic Details
Author: Pinto, Alessandro
Format: doctoral thesis
Status:Published version
Publication Date:2012
Country:España
Institution:CBUC, CESCA
Repository:TDR. Tesis Doctorales en Red
OAI Identifier:oai:www.tdx.cat:10803/80744
Online Access:http://hdl.handle.net/10803/80744
Access Level:Open access
Keyword:Aptamer
aptaPCR
QPCR
Thrombin
gliadin
543
577
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spelling Real-time aptapcr: a novel approach exploiting nucleic acid aptamers for ultrasensitive detection of analytes for clinical diagnostic and in food analysisPinto, AlessandroAptameraptaPCRQPCRThrombingliadin543577The thesis aimed to develop and characterize a novel detection approach, which we termed aptaPCR exploiting nucleic acid aptamers as combined recognition and reporter biocomponents for the ultrasensitive detection of analytes. Nucleic acid aptamers are synthetic ligands selected from vast combinatorial libraries through a process referred to as SELEX – Systematic Evolution of Ligand By Exponential Enrichment. As compared to other natural and synthetic receptor, aptamers possess unique chemical and biochemical characteristics, such as: a well known chemistry, remarkable stability, an ability to be selected against virtually any target even in non-physiological conditionsUniversitat Rovira i VirgiliO'sullivan, CiaraUniversitat Rovira i Virgili. Departament d'Enginyeria Química201220122012info:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/publishedVersion116 p.application/pdfapplication/pdfhttp://hdl.handle.net/10803/80744TDX (Tesis Doctorals en Xarxa)reponame:TDR. Tesis Doctorales en Redinstname:CBUC, CESCAInglésADVERTIMENT. L'accés als continguts d'aquesta tesi doctoral i la seva utilització ha de respectar els drets de la persona autora. Pot ser utilitzada per a consulta o estudi personal, així com en activitats o materials d'investigació i docència en els termes establerts a l'art. 32 del Text Refós de la Llei de Propietat Intel·lectual (RDL 1/1996). Per altres utilitzacions es requereix l'autorització prèvia i expressa de la persona autora. En qualsevol cas, en la utilització dels seus continguts caldrà indicar de forma clara el nom i cognoms de la persona autora i el títol de la tesi doctoral. No s'autoritza la seva reproducció o altres formes d'explotació efectuades amb finalitats de lucre ni la seva comunicació pública des d'un lloc aliè al servei TDX. Tampoc s'autoritza la presentació del seu contingut en una finestra o marc aliè a TDX (framing). Aquesta reserva de drets afecta tant als continguts de la tesi com als seus resums i índexs.info:eu-repo/semantics/openAccessoai:www.tdx.cat:10803/807442026-06-14T12:46:07Z
dc.title.none.fl_str_mv Real-time aptapcr: a novel approach exploiting nucleic acid aptamers for ultrasensitive detection of analytes for clinical diagnostic and in food analysis
title Real-time aptapcr: a novel approach exploiting nucleic acid aptamers for ultrasensitive detection of analytes for clinical diagnostic and in food analysis
spellingShingle Real-time aptapcr: a novel approach exploiting nucleic acid aptamers for ultrasensitive detection of analytes for clinical diagnostic and in food analysis
Pinto, Alessandro
Aptamer
aptaPCR
QPCR
Thrombin
gliadin
543
577
title_short Real-time aptapcr: a novel approach exploiting nucleic acid aptamers for ultrasensitive detection of analytes for clinical diagnostic and in food analysis
title_full Real-time aptapcr: a novel approach exploiting nucleic acid aptamers for ultrasensitive detection of analytes for clinical diagnostic and in food analysis
title_fullStr Real-time aptapcr: a novel approach exploiting nucleic acid aptamers for ultrasensitive detection of analytes for clinical diagnostic and in food analysis
title_full_unstemmed Real-time aptapcr: a novel approach exploiting nucleic acid aptamers for ultrasensitive detection of analytes for clinical diagnostic and in food analysis
title_sort Real-time aptapcr: a novel approach exploiting nucleic acid aptamers for ultrasensitive detection of analytes for clinical diagnostic and in food analysis
dc.creator.none.fl_str_mv Pinto, Alessandro
author Pinto, Alessandro
author_facet Pinto, Alessandro
author_role author
dc.contributor.none.fl_str_mv O'sullivan, Ciara
Universitat Rovira i Virgili. Departament d'Enginyeria Química
dc.subject.none.fl_str_mv Aptamer
aptaPCR
QPCR
Thrombin
gliadin
543
577
topic Aptamer
aptaPCR
QPCR
Thrombin
gliadin
543
577
description The thesis aimed to develop and characterize a novel detection approach, which we termed aptaPCR exploiting nucleic acid aptamers as combined recognition and reporter biocomponents for the ultrasensitive detection of analytes. Nucleic acid aptamers are synthetic ligands selected from vast combinatorial libraries through a process referred to as SELEX – Systematic Evolution of Ligand By Exponential Enrichment. As compared to other natural and synthetic receptor, aptamers possess unique chemical and biochemical characteristics, such as: a well known chemistry, remarkable stability, an ability to be selected against virtually any target even in non-physiological conditions
publishDate 2012
dc.date.none.fl_str_mv 2012
2012
2012
dc.type.none.fl_str_mv info:eu-repo/semantics/doctoralThesis
info:eu-repo/semantics/publishedVersion
format doctoralThesis
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10803/80744
url http://hdl.handle.net/10803/80744
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 116 p.
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Universitat Rovira i Virgili
publisher.none.fl_str_mv Universitat Rovira i Virgili
dc.source.none.fl_str_mv TDX (Tesis Doctorals en Xarxa)
reponame:TDR. Tesis Doctorales en Red
instname:CBUC, CESCA
instname_str CBUC, CESCA
reponame_str TDR. Tesis Doctorales en Red
collection TDR. Tesis Doctorales en Red
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869424995812245504
score 15,300719

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