Molecular Epidemiology of Staphylococcus aureus Bacteremia: Association of Molecular Factors With the Source of Infection

Staphylococcus aureus bacteremia (SAB) is associated with high morbidity and mortality, which varies depending on the source of infection. Nevertheless, the global molecular epidemiology of SAB and its possible association with specific virulence factors remains unclear. Using DNA microarrays, a tot...

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Autores: Perez-Montarelo, Dame, Viedma, Esther, Larrosa, Nieves, Gomez-Gonzalez, Carmen, Ruiz de Gopegui-Bordes, Enrique, Munoz-Gallego, Irene, San Juan, Rafael, Fernandez-Hidalgo, Nuria, Aimirante, Benito, Chaves, Fernando
Formato: artículo
Fecha de publicación:2018
País:España
Recursos:Instituto de Salud Carlos III (ISCIII)
Repositorio:Repisalud
Idioma:inglés
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/22568
Acesso em linha:https://hdl.handle.net/20.500.12105/22568
Access Level:acceso abierto
Palavra-chave:Staphylococcus aureus bacteremia
Bacteremia source
Molecular epidemiology
Clonal complex
Virulence factors
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spelling Molecular Epidemiology of Staphylococcus aureus Bacteremia: Association of Molecular Factors With the Source of InfectionPerez-Montarelo, DameViedma, EstherLarrosa, NievesGomez-Gonzalez, CarmenRuiz de Gopegui-Bordes, EnriqueMunoz-Gallego, IreneSan Juan, RafaelFernandez-Hidalgo, NuriaAimirante, BenitoChaves, FernandoStaphylococcus aureus bacteremiaBacteremia sourceMolecular epidemiologyClonal complexVirulence factorsStaphylococcus aureus bacteremia (SAB) is associated with high morbidity and mortality, which varies depending on the source of infection. Nevertheless, the global molecular epidemiology of SAB and its possible association with specific virulence factors remains unclear. Using DNA microarrays, a total of 833 S. aureus strains (785 SAB and 48 colonizing strains) collected in Spain over a period of 15 years (2002-2017) were characterized to determine clonal complex (CC), agr type and repertoire of resistance and virulence genes in order to provide an epidemiological overview of CCs causing bloodstream infection, and to analyze possible associations between virulence genes and the most common sources of bacteremia. The results were also analyzed by acquisition (healthcare-associated [HA] and community-acquired [CA]), methicillin-resistant (MRSA) and methicillin-susceptible (MSSA) strains, and patient age (adults vs. children). Our results revealed high clonal diversity among SAB strains with up to 28 different CCs. The most prevalent CCs were CC5 (30.8%), CC30 (20.3%), CC45 (8.3%), CC8 (8.4%), CC15 (7.5%), and CC22 (5.9%), which together accounted for 80% of all cases. A higher proportion of CC5 was found among HA strains than CA strains (35.6 vs. 20.2%, p < 0.001). CC5 was associated with methicillin resistance (14.7 vs. 79.4%, p < 0.001), whereas CC30, CC45, and CC15 were correlated with MSSA strains (p < 0.001). Pathogen-related molecular markers significantly associated with a specific source of bacteremia included the presence of sea, undisrupted hlb and isaB genes with catheter-related bacteremia; sed, splE, and fib genes with endocarditis; undisrupted hlb with skin and soft tissue infections; and finally, CC5, msrA resistance gene and hla gene with osteoarticular source. Our study suggests an association between S. aureus genotype and place of acquisition, methicillin resistance and sources of bloodstream infection, and provides a valuable starting point for further research insights into intrinsic pathogenic mechanisms involved in the development of SAB.Frontiers Media20242024-09-0620182018-09-2520182018-09-25research articlehttp://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articlehttps://hdl.handle.net/20.500.12105/22568reponame:Repisaludinstname:Instituto de Salud Carlos III (ISCIII)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repisalud.isciii.es:20.500.12105/225682026-06-12T12:43:37Z
dc.title.none.fl_str_mv Molecular Epidemiology of Staphylococcus aureus Bacteremia: Association of Molecular Factors With the Source of Infection
title Molecular Epidemiology of Staphylococcus aureus Bacteremia: Association of Molecular Factors With the Source of Infection
spellingShingle Molecular Epidemiology of Staphylococcus aureus Bacteremia: Association of Molecular Factors With the Source of Infection
Perez-Montarelo, Dame
Staphylococcus aureus bacteremia
Bacteremia source
Molecular epidemiology
Clonal complex
Virulence factors
title_short Molecular Epidemiology of Staphylococcus aureus Bacteremia: Association of Molecular Factors With the Source of Infection
title_full Molecular Epidemiology of Staphylococcus aureus Bacteremia: Association of Molecular Factors With the Source of Infection
title_fullStr Molecular Epidemiology of Staphylococcus aureus Bacteremia: Association of Molecular Factors With the Source of Infection
title_full_unstemmed Molecular Epidemiology of Staphylococcus aureus Bacteremia: Association of Molecular Factors With the Source of Infection
title_sort Molecular Epidemiology of Staphylococcus aureus Bacteremia: Association of Molecular Factors With the Source of Infection
dc.creator.none.fl_str_mv Perez-Montarelo, Dame
Viedma, Esther
Larrosa, Nieves
Gomez-Gonzalez, Carmen
Ruiz de Gopegui-Bordes, Enrique
Munoz-Gallego, Irene
San Juan, Rafael
Fernandez-Hidalgo, Nuria
Aimirante, Benito
Chaves, Fernando
author Perez-Montarelo, Dame
author_facet Perez-Montarelo, Dame
Viedma, Esther
Larrosa, Nieves
Gomez-Gonzalez, Carmen
Ruiz de Gopegui-Bordes, Enrique
Munoz-Gallego, Irene
San Juan, Rafael
Fernandez-Hidalgo, Nuria
Aimirante, Benito
Chaves, Fernando
author_role author
author2 Viedma, Esther
Larrosa, Nieves
Gomez-Gonzalez, Carmen
Ruiz de Gopegui-Bordes, Enrique
Munoz-Gallego, Irene
San Juan, Rafael
Fernandez-Hidalgo, Nuria
Aimirante, Benito
Chaves, Fernando
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv
dc.subject.none.fl_str_mv Staphylococcus aureus bacteremia
Bacteremia source
Molecular epidemiology
Clonal complex
Virulence factors
topic Staphylococcus aureus bacteremia
Bacteremia source
Molecular epidemiology
Clonal complex
Virulence factors
description Staphylococcus aureus bacteremia (SAB) is associated with high morbidity and mortality, which varies depending on the source of infection. Nevertheless, the global molecular epidemiology of SAB and its possible association with specific virulence factors remains unclear. Using DNA microarrays, a total of 833 S. aureus strains (785 SAB and 48 colonizing strains) collected in Spain over a period of 15 years (2002-2017) were characterized to determine clonal complex (CC), agr type and repertoire of resistance and virulence genes in order to provide an epidemiological overview of CCs causing bloodstream infection, and to analyze possible associations between virulence genes and the most common sources of bacteremia. The results were also analyzed by acquisition (healthcare-associated [HA] and community-acquired [CA]), methicillin-resistant (MRSA) and methicillin-susceptible (MSSA) strains, and patient age (adults vs. children). Our results revealed high clonal diversity among SAB strains with up to 28 different CCs. The most prevalent CCs were CC5 (30.8%), CC30 (20.3%), CC45 (8.3%), CC8 (8.4%), CC15 (7.5%), and CC22 (5.9%), which together accounted for 80% of all cases. A higher proportion of CC5 was found among HA strains than CA strains (35.6 vs. 20.2%, p < 0.001). CC5 was associated with methicillin resistance (14.7 vs. 79.4%, p < 0.001), whereas CC30, CC45, and CC15 were correlated with MSSA strains (p < 0.001). Pathogen-related molecular markers significantly associated with a specific source of bacteremia included the presence of sea, undisrupted hlb and isaB genes with catheter-related bacteremia; sed, splE, and fib genes with endocarditis; undisrupted hlb with skin and soft tissue infections; and finally, CC5, msrA resistance gene and hla gene with osteoarticular source. Our study suggests an association between S. aureus genotype and place of acquisition, methicillin resistance and sources of bloodstream infection, and provides a valuable starting point for further research insights into intrinsic pathogenic mechanisms involved in the development of SAB.
publishDate 2018
dc.date.none.fl_str_mv 2018
2018-09-25
2018
2018-09-25
2024
2024-09-06
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.12105/22568
url https://hdl.handle.net/20.500.12105/22568
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repisalud
instname:Instituto de Salud Carlos III (ISCIII)
instname_str Instituto de Salud Carlos III (ISCIII)
reponame_str Repisalud
collection Repisalud
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812455

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