TMEM95 is a sperm membrane protein essential for mammalian fertilization

18 Pág.

Bibliographic Details
Authors: Lamas Toranzo, Ismael, Hamzé, Julieta G., Bianchi, Enrica, Fernández-Fuertes, Beatriz, Pérez Cerezales, Serafín, Laguna-Barraza, Ricardo, Fernández González, Raúl, Lonergan, P., Gutiérrez-Adán, Alfonso, Wright, Gavin J., Jiménez-Movilla, Maria, Bermejo Álvarez, Pablo
Format: article
Status:Published version
Publication Date:2020
Country:España
Institution:Consejo Superior de Investigaciones Científicas (CSIC)
Repository:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/340386
Online Access:http://hdl.handle.net/10261/340386
https://api.elsevier.com/content/abstract/scopus_id/85086524244
Access Level:Open access
Keyword:IZUMO
JUNO
Cell biology
Developmental biology
Fertilization
Mouse
Reproduction
id ES_f83abd1772809e998f3edd2dca142574
oai_identifier_str oai:digital.csic.es:10261/340386
network_acronym_str ES
network_name_str España
repository_id_str
spelling TMEM95 is a sperm membrane protein essential for mammalian fertilizationLamas Toranzo, IsmaelHamzé, Julieta G.Bianchi, EnricaFernández-Fuertes, BeatrizPérez Cerezales, SerafínLaguna-Barraza, RicardoFernández González, RaúlLonergan, P.Gutiérrez-Adán, AlfonsoWright, Gavin J.Jiménez-Movilla, MariaBermejo Álvarez, PabloIZUMOJUNOCell biologyDevelopmental biologyFertilizationMouseReproduction18 Pág.The fusion of gamete membranes during fertilization is an essential process for sexual reproduction. Despite its importance, only three proteins are known to be indispensable for sperm-egg membrane fusion: the sperm proteins IZUMO1 and SPACA6, and the egg protein JUNO. Here we demonstrate that another sperm protein, TMEM95, is necessary for sperm-egg interaction. TMEM95 ablation in mice caused complete male-specific infertility. Sperm lacking this protein were morphologically normal exhibited normal motility, and could penetrate the zona pellucida and bind to the oolemma. However, once bound to the oolemma, TMEM95-deficient sperm were unable to fuse with the egg membrane or penetrate into the ooplasm, and fertilization could only be achieved by mechanical injection of one sperm into the ooplasm, thereby bypassing membrane fusion. These data demonstrate that TMEM95 is essential for mammalian fertilization.Funding was provided by the Spanish Ministry of Science and Competitiveness (RYC-2012-10193, AGL2014- 58739-R and AGL2017-84908-R to PBA, AGL2015-70159-P to MJM, RTI2018-093548-B-I00 to AGA and the network project AGL2016-71890-REDT), European Research Council (StG 757886-ELON- GAN to PBA), Fundación Seneca-Agencia de Ciencia y Tecnología de Murcia (20887/PI/18 to MJM), the Irish Department of Agriculture, Food and The Marine (11/S/104), and the United Kingdom Medical Research Council (MR/M012468/1 to EB and GJW). ILT and SPC were supported by FPI and Ramón y Cajal contracts, respectively, from the Spanish Ministry of Science and Competitiveness. BFF is supported by a Marie Curie Fellowship from the European Commission.Departamento de Reproducción Animal​ Peer reviewedeLife Sciences PublicationsMinisterio de Economía y Competitividad (España)Agencia Estatal de Investigación (España)European CommissionFundación SénecaIrish GovernmentMedical Research Council (UK)Lamas Toranzo, Ismael [0000-0002-7790-2649]Hamzé, Julieta G. [0000-0002-9742-8614]Bianchi, Enrica [0000-0001-8124-7328]Fernández-Fuertes, Beatriz [0000-0003-2303-8733]Pérez Cerezales, Serafín [0000-0003-2119-6228]Fernández González, Raúl [0000-0003-1989-2945]Gutiérrez-Adán, Alfonso [0000-0001-9893-9179]Wright, Gavin J. [0000-0003-0537-0863]Jiménez-Movilla, Maria [0000-0002-1572-8219]Bermejo Álvarez, Pablo [0000-0001-9907-2626]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/340386https://api.elsevier.com/content/abstract/scopus_id/85086524244reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO//RYC-2012-10193info:eu-repo/grantAgreement/MINECO//AGL2014-58739-Rinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-84908-Rinfo:eu-repo/grantAgreement/MINECO//AGL2015-70159-Pinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-093548-B-I00info:eu-repo/grantAgreement/AEI//AGL2016-71890-REDTinfo:eu-repo/grantAgreement/EC/757886/eLifehttps://doi.org/10.7554/eLife.53913Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3403862026-05-22T06:33:51Z
dc.title.none.fl_str_mv TMEM95 is a sperm membrane protein essential for mammalian fertilization
title TMEM95 is a sperm membrane protein essential for mammalian fertilization
spellingShingle TMEM95 is a sperm membrane protein essential for mammalian fertilization
Lamas Toranzo, Ismael
IZUMO
JUNO
Cell biology
Developmental biology
Fertilization
Mouse
Reproduction
title_short TMEM95 is a sperm membrane protein essential for mammalian fertilization
title_full TMEM95 is a sperm membrane protein essential for mammalian fertilization
title_fullStr TMEM95 is a sperm membrane protein essential for mammalian fertilization
title_full_unstemmed TMEM95 is a sperm membrane protein essential for mammalian fertilization
title_sort TMEM95 is a sperm membrane protein essential for mammalian fertilization
dc.creator.none.fl_str_mv Lamas Toranzo, Ismael
Hamzé, Julieta G.
Bianchi, Enrica
Fernández-Fuertes, Beatriz
Pérez Cerezales, Serafín
Laguna-Barraza, Ricardo
Fernández González, Raúl
Lonergan, P.
Gutiérrez-Adán, Alfonso
Wright, Gavin J.
Jiménez-Movilla, Maria
Bermejo Álvarez, Pablo
author Lamas Toranzo, Ismael
author_facet Lamas Toranzo, Ismael
Hamzé, Julieta G.
Bianchi, Enrica
Fernández-Fuertes, Beatriz
Pérez Cerezales, Serafín
Laguna-Barraza, Ricardo
Fernández González, Raúl
Lonergan, P.
Gutiérrez-Adán, Alfonso
Wright, Gavin J.
Jiménez-Movilla, Maria
Bermejo Álvarez, Pablo
author_role author
author2 Hamzé, Julieta G.
Bianchi, Enrica
Fernández-Fuertes, Beatriz
Pérez Cerezales, Serafín
Laguna-Barraza, Ricardo
Fernández González, Raúl
Lonergan, P.
Gutiérrez-Adán, Alfonso
Wright, Gavin J.
Jiménez-Movilla, Maria
Bermejo Álvarez, Pablo
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Agencia Estatal de Investigación (España)
European Commission
Fundación Séneca
Irish Government
Medical Research Council (UK)
Lamas Toranzo, Ismael [0000-0002-7790-2649]
Hamzé, Julieta G. [0000-0002-9742-8614]
Bianchi, Enrica [0000-0001-8124-7328]
Fernández-Fuertes, Beatriz [0000-0003-2303-8733]
Pérez Cerezales, Serafín [0000-0003-2119-6228]
Fernández González, Raúl [0000-0003-1989-2945]
Gutiérrez-Adán, Alfonso [0000-0001-9893-9179]
Wright, Gavin J. [0000-0003-0537-0863]
Jiménez-Movilla, Maria [0000-0002-1572-8219]
Bermejo Álvarez, Pablo [0000-0001-9907-2626]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv IZUMO
JUNO
Cell biology
Developmental biology
Fertilization
Mouse
Reproduction
topic IZUMO
JUNO
Cell biology
Developmental biology
Fertilization
Mouse
Reproduction
description 18 Pág.
publishDate 2020
dc.date.none.fl_str_mv 2020
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/340386
https://api.elsevier.com/content/abstract/scopus_id/85086524244
url http://hdl.handle.net/10261/340386
https://api.elsevier.com/content/abstract/scopus_id/85086524244
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO//RYC-2012-10193
info:eu-repo/grantAgreement/MINECO//AGL2014-58739-R
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-84908-R
info:eu-repo/grantAgreement/MINECO//AGL2015-70159-P
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-093548-B-I00
info:eu-repo/grantAgreement/AEI//AGL2016-71890-REDT
info:eu-repo/grantAgreement/EC/757886/
eLife
https://doi.org/10.7554/eLife.53913
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv eLife Sciences Publications
publisher.none.fl_str_mv eLife Sciences Publications
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869424982583410688
score 15.811543

Similar Items