Antioxidant and anti-genotoxic properties of cerium oxide nanoparticles in a pulmonary-like cell system

Cerium oxide nanoparticles (CeO2-NP) present two different oxidation states what can suppose an auto-regenerative redox cycle. Potential applications of CeO2-NP to quench reactive oxygen species (ROS) in biological systems are currently being investigated. In this context, CeO2-NP may represent a no...

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Autores: Rubio Lorente, Laura|||0000-0002-2088-3803, Annangi, Balasubramanyam|||0000-0002-1479-138X, Vila Vecilla, Laura|||0000-0001-5573-4886, Hernández Bonilla, Alba|||0000-0001-6938-1233, Marcos Dauder, Ricardo|||0000-0001-7891-357X
Tipo de recurso: artículo
Fecha de publicación:2016
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:308398
Acceso en línea:https://ddd.uab.cat/record/308398
https://dx.doi.org/urn:doi:10.1007/s00204-015-1468-y
Access Level:acceso abierto
Palabra clave:Antigenotoxic
Antioxidant
BEAS-2B cells
Cerium oxide nanoparticles
Oxidative stress
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spelling Antioxidant and anti-genotoxic properties of cerium oxide nanoparticles in a pulmonary-like cell systemRubio Lorente, Laura|||0000-0002-2088-3803Annangi, Balasubramanyam|||0000-0002-1479-138XVila Vecilla, Laura|||0000-0001-5573-4886Hernández Bonilla, Alba|||0000-0001-6938-1233Marcos Dauder, Ricardo|||0000-0001-7891-357XAntigenotoxicAntioxidantBEAS-2B cellsCerium oxide nanoparticlesOxidative stressCerium oxide nanoparticles (CeO2-NP) present two different oxidation states what can suppose an auto-regenerative redox cycle. Potential applications of CeO2-NP to quench reactive oxygen species (ROS) in biological systems are currently being investigated. In this context, CeO2-NP may represent a novel agent to protect cells and tissues against oxidative damage by its regenerative free radical-scavenging properties. In this study, we have used a human epithelial lung cell line, BEAS-2B, as a model to study the possible antioxidant and anti-genotoxic effect of CeO2-NP in a pulmonary-like system. We have assessed the protective effect of CeO2-NP pre-treatment in front of a well-defined oxidative stress-inducing agent (KBrO3). Different endpoints like toxicity, intracellular ROS induction, genotoxicity and DNA oxidative damage (comet assay), and gene expression alterations have been evaluated. The obtained results confirmed the antioxidant properties of CeO2-NP. Thus, its pre-treatment significantly reduced the intracellular production of ROS induced by KBrO3. Similarly, a reduction in the levels of DNA oxidative damage, as measured with the comet assay complemented with formamidopyrimidine DNA glycosylase enzyme, was also observed. Pre-treatment of BEAS-2B cells with CeO2-NP (at 2.5 µg/mL) slightly increased the viability of cells treated with KBrO3 as well as down-regulated the expression of the Ho1 and Sod2 genes involved in the oxidative Nrf2 pathway. Our finding would support the potential usefulness of CeO2-NP as a pharmacological agent to be used against diseases caused by oxidative stress.Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia 22016-01-0120162016-01-01Articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/308398https://dx.doi.org/urn:doi:10.1007/s00204-015-1468-yreponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengAgència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2014/SGR-202open accesshttp://purl.org/coar/access_right/c_abf2Aquest material està protegit per drets d'autor i/o drets afins. Podeu utilitzar aquest material en funció del que permet la legislació de drets d'autor i drets afins d'aplicació al vostre cas. Per a d'altres usos heu d'obtenir permís del(s) titular(s) de drets.https://rightsstatements.org/vocab/InC/1.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:3083982026-06-06T12:50:31Z
dc.title.none.fl_str_mv Antioxidant and anti-genotoxic properties of cerium oxide nanoparticles in a pulmonary-like cell system
title Antioxidant and anti-genotoxic properties of cerium oxide nanoparticles in a pulmonary-like cell system
spellingShingle Antioxidant and anti-genotoxic properties of cerium oxide nanoparticles in a pulmonary-like cell system
Rubio Lorente, Laura|||0000-0002-2088-3803
Antigenotoxic
Antioxidant
BEAS-2B cells
Cerium oxide nanoparticles
Oxidative stress
title_short Antioxidant and anti-genotoxic properties of cerium oxide nanoparticles in a pulmonary-like cell system
title_full Antioxidant and anti-genotoxic properties of cerium oxide nanoparticles in a pulmonary-like cell system
title_fullStr Antioxidant and anti-genotoxic properties of cerium oxide nanoparticles in a pulmonary-like cell system
title_full_unstemmed Antioxidant and anti-genotoxic properties of cerium oxide nanoparticles in a pulmonary-like cell system
title_sort Antioxidant and anti-genotoxic properties of cerium oxide nanoparticles in a pulmonary-like cell system
dc.creator.none.fl_str_mv Rubio Lorente, Laura|||0000-0002-2088-3803
Annangi, Balasubramanyam|||0000-0002-1479-138X
Vila Vecilla, Laura|||0000-0001-5573-4886
Hernández Bonilla, Alba|||0000-0001-6938-1233
Marcos Dauder, Ricardo|||0000-0001-7891-357X
author Rubio Lorente, Laura|||0000-0002-2088-3803
author_facet Rubio Lorente, Laura|||0000-0002-2088-3803
Annangi, Balasubramanyam|||0000-0002-1479-138X
Vila Vecilla, Laura|||0000-0001-5573-4886
Hernández Bonilla, Alba|||0000-0001-6938-1233
Marcos Dauder, Ricardo|||0000-0001-7891-357X
author_role author
author2 Annangi, Balasubramanyam|||0000-0002-1479-138X
Vila Vecilla, Laura|||0000-0001-5573-4886
Hernández Bonilla, Alba|||0000-0001-6938-1233
Marcos Dauder, Ricardo|||0000-0001-7891-357X
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia
dc.subject.none.fl_str_mv Antigenotoxic
Antioxidant
BEAS-2B cells
Cerium oxide nanoparticles
Oxidative stress
topic Antigenotoxic
Antioxidant
BEAS-2B cells
Cerium oxide nanoparticles
Oxidative stress
description Cerium oxide nanoparticles (CeO2-NP) present two different oxidation states what can suppose an auto-regenerative redox cycle. Potential applications of CeO2-NP to quench reactive oxygen species (ROS) in biological systems are currently being investigated. In this context, CeO2-NP may represent a novel agent to protect cells and tissues against oxidative damage by its regenerative free radical-scavenging properties. In this study, we have used a human epithelial lung cell line, BEAS-2B, as a model to study the possible antioxidant and anti-genotoxic effect of CeO2-NP in a pulmonary-like system. We have assessed the protective effect of CeO2-NP pre-treatment in front of a well-defined oxidative stress-inducing agent (KBrO3). Different endpoints like toxicity, intracellular ROS induction, genotoxicity and DNA oxidative damage (comet assay), and gene expression alterations have been evaluated. The obtained results confirmed the antioxidant properties of CeO2-NP. Thus, its pre-treatment significantly reduced the intracellular production of ROS induced by KBrO3. Similarly, a reduction in the levels of DNA oxidative damage, as measured with the comet assay complemented with formamidopyrimidine DNA glycosylase enzyme, was also observed. Pre-treatment of BEAS-2B cells with CeO2-NP (at 2.5 µg/mL) slightly increased the viability of cells treated with KBrO3 as well as down-regulated the expression of the Ho1 and Sod2 genes involved in the oxidative Nrf2 pathway. Our finding would support the potential usefulness of CeO2-NP as a pharmacological agent to be used against diseases caused by oxidative stress.
publishDate 2016
dc.date.none.fl_str_mv 2
2016-01-01
2016
2016-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/308398
https://dx.doi.org/urn:doi:10.1007/s00204-015-1468-y
url https://ddd.uab.cat/record/308398
https://dx.doi.org/urn:doi:10.1007/s00204-015-1468-y
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2014/SGR-202
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://rightsstatements.org/vocab/InC/1.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://rightsstatements.org/vocab/InC/1.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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