Generation of magnetized olfactory ensheathing cells for regenerative studies in the central and peripheral nervous tissue

This article belongs to the Special Issue Magnetic Nanoparticles 2013.

Detalles Bibliográficos
Autores: Riggio, Cristina, Calatayud, M. Pilar, Goya, Gerardo F., Del Río, José Antonio
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2013
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/367530
Acceso en línea:http://hdl.handle.net/10261/367530
Access Level:acceso abierto
Palabra clave:Magnetic nanoparticles
Organotypic culture
Nerve regeneration
Olfactory ensheathing cell
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spelling Generation of magnetized olfactory ensheathing cells for regenerative studies in the central and peripheral nervous tissueRiggio, CristinaCalatayud, M. PilarGoya, Gerardo F.Del Río, José AntonioMagnetic nanoparticlesOrganotypic cultureNerve regenerationOlfactory ensheathing cellThis article belongs to the Special Issue Magnetic Nanoparticles 2013.As olfactory receptor axons grow from the peripheral to the central nervous system (CNS) aided by olfactory ensheathing cells (OECs), the transplantation of OECs has been suggested as a plausible therapy for spinal cord lesions. The problem with this hypothesis is that OECs do not represent a single homogeneous entity, but, instead, a functionally heterogeneous population that exhibits a variety of responses, including adhesion and repulsion during cell-matrix interactions. Some studies report that the migratory properties of OECs are compromised by inhibitory molecules and potentiated by chemical gradients. In this paper, we report a system based on modified OECs carrying magnetic nanoparticles as a proof of concept experiment enabling specific studies aimed at exploring the potential of OECs in the treatment of spinal cord injuries. Our studies have confirmed that magnetized OECs (i) survive well without exhibiting stress-associated cellular responses; (ii) in vitro, their migration can be modulated by magnetic fields; and (iii) their transplantation in organotypic slices of spinal cord and peripheral nerve showed positive integration in the model. Altogether, these findings indicate the therapeutic potential of magnetized OECs for CNS injuries.This work was partially supported by Cassa di Risparmio di Pisa Foundation (MDIAB project), NanoSciE+ transnational call of EC FP7 (MARVENE project), FP7-PRIORITY (grant number 278486) and DEMTEST (Joint Programming of Neurodegenerative Diseases, PI11/03028), the Spanish Ministry of Science and Innovation (MINECO, MAT2010-19326 and BFU2012-32617), Generalitat de Catalunya (SGR2009-366), La Caixa Obra Social Foundation and the Instituto Salud Carlos III to J.A.D.R., S.N. was supported by MICINN.Peer reviewedMultidisciplinary Digital Publishing InstituteFondazione PisaEuropean CommissionMinisterio de Ciencia e Innovación (España)Instituto de Salud Carlos IIIGeneralitat de CatalunyaMinisterio de Economía y Competitividad (España)Fundación la Caixa202420242013info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/367530reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésThe underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.3390/ijms140610852https://doi.org/10.3390/ijms140610852Noinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3675302026-05-22T06:33:51Z
dc.title.none.fl_str_mv Generation of magnetized olfactory ensheathing cells for regenerative studies in the central and peripheral nervous tissue
title Generation of magnetized olfactory ensheathing cells for regenerative studies in the central and peripheral nervous tissue
spellingShingle Generation of magnetized olfactory ensheathing cells for regenerative studies in the central and peripheral nervous tissue
Riggio, Cristina
Magnetic nanoparticles
Organotypic culture
Nerve regeneration
Olfactory ensheathing cell
title_short Generation of magnetized olfactory ensheathing cells for regenerative studies in the central and peripheral nervous tissue
title_full Generation of magnetized olfactory ensheathing cells for regenerative studies in the central and peripheral nervous tissue
title_fullStr Generation of magnetized olfactory ensheathing cells for regenerative studies in the central and peripheral nervous tissue
title_full_unstemmed Generation of magnetized olfactory ensheathing cells for regenerative studies in the central and peripheral nervous tissue
title_sort Generation of magnetized olfactory ensheathing cells for regenerative studies in the central and peripheral nervous tissue
dc.creator.none.fl_str_mv Riggio, Cristina
Calatayud, M. Pilar
Goya, Gerardo F.
Del Río, José Antonio
author Riggio, Cristina
author_facet Riggio, Cristina
Calatayud, M. Pilar
Goya, Gerardo F.
Del Río, José Antonio
author_role author
author2 Calatayud, M. Pilar
Goya, Gerardo F.
Del Río, José Antonio
author2_role author
author
author
dc.contributor.none.fl_str_mv Fondazione Pisa
European Commission
Ministerio de Ciencia e Innovación (España)
Instituto de Salud Carlos III
Generalitat de Catalunya
Ministerio de Economía y Competitividad (España)
Fundación la Caixa
dc.subject.none.fl_str_mv Magnetic nanoparticles
Organotypic culture
Nerve regeneration
Olfactory ensheathing cell
topic Magnetic nanoparticles
Organotypic culture
Nerve regeneration
Olfactory ensheathing cell
description This article belongs to the Special Issue Magnetic Nanoparticles 2013.
publishDate 2013
dc.date.none.fl_str_mv 2013
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/367530
url http://hdl.handle.net/10261/367530
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.3390/ijms140610852
https://doi.org/10.3390/ijms140610852
No
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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