Zur (FurB) is a key factor in the control of the oxidative stress response in Anabaena sp. PCC 7120.

Iron and zinc are necessary nutrients whose homeostasis is tightly controlled by members of the ferric uptake regulator (FUR) superfamily in the cyanobacterium Anabaena sp. PCC7120. Although the link between iron metabolism and oxidative stress management is well documented, little is known about th...

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Detalles Bibliográficos
Autores: Sein-Echaluce, Violeta C., González, Andrés, Napolitano, Mauro, Luque, Ignacio, Barja, Francisco, Peleato, M.L., Fillat, María F.
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2015
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/117692
Acceso en línea:http://hdl.handle.net/10261/117692
Access Level:acceso abierto
Palabra clave:Bacteria
Ecophysiology
Environmental signal/stress responses
Gene expression/regulation
Metabolic networks
Metals
Microbial genetics
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spelling Zur (FurB) is a key factor in the control of the oxidative stress response in Anabaena sp. PCC 7120.Sein-Echaluce, Violeta C.González, AndrésNapolitano, MauroLuque, IgnacioBarja, FranciscoPeleato, M.L.Fillat, María F.BacteriaEcophysiologyEnvironmental signal/stress responsesGene expression/regulationMetabolic networksMetalsMicrobial geneticsIron and zinc are necessary nutrients whose homeostasis is tightly controlled by members of the ferric uptake regulator (FUR) superfamily in the cyanobacterium Anabaena sp. PCC7120. Although the link between iron metabolism and oxidative stress management is well documented, little is known about the connection between zinc homeostasis and the oxidative stress response in cyanobacteria. Zinc homeostasis in Anabaena is controlled by Zur, also named FurB. When overexpressed in Escherichia coli, Zur (FurB) improved cell survival during oxidative stress. In order to investigate the possible correlation between Zur and the oxidative stress response in Anabaena, zur deletion and zur-overexpressing strains have been constructed, and the consequences of Zur imbalance evaluated. The lack of Zur increased sensitivity to hydrogen peroxide (H2 O2 ), whereas an excess of Zur enhanced oxidative stress resistance. Both mutants displayed pleiotropic phenotypes, including alterations on the filament surfaces observable by scanning electron microscopy, reduced content of endogenous H2 O2 and altered expression of sodA, catalases and several peroxiredoxins. Transcriptional and biochemical analyses unveiled that the appropriate level of Zur is required for proper control of the oxidative stress response and allowed us to identify major antioxidant enzymes as novel members of the Zur regulon.Peer reviewedWiley-BlackwellConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201520152015info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/117692reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1111/1462-2920.12628Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1176922026-05-22T06:33:51Z
dc.title.none.fl_str_mv Zur (FurB) is a key factor in the control of the oxidative stress response in Anabaena sp. PCC 7120.
title Zur (FurB) is a key factor in the control of the oxidative stress response in Anabaena sp. PCC 7120.
spellingShingle Zur (FurB) is a key factor in the control of the oxidative stress response in Anabaena sp. PCC 7120.
Sein-Echaluce, Violeta C.
Bacteria
Ecophysiology
Environmental signal/stress responses
Gene expression/regulation
Metabolic networks
Metals
Microbial genetics
title_short Zur (FurB) is a key factor in the control of the oxidative stress response in Anabaena sp. PCC 7120.
title_full Zur (FurB) is a key factor in the control of the oxidative stress response in Anabaena sp. PCC 7120.
title_fullStr Zur (FurB) is a key factor in the control of the oxidative stress response in Anabaena sp. PCC 7120.
title_full_unstemmed Zur (FurB) is a key factor in the control of the oxidative stress response in Anabaena sp. PCC 7120.
title_sort Zur (FurB) is a key factor in the control of the oxidative stress response in Anabaena sp. PCC 7120.
dc.creator.none.fl_str_mv Sein-Echaluce, Violeta C.
González, Andrés
Napolitano, Mauro
Luque, Ignacio
Barja, Francisco
Peleato, M.L.
Fillat, María F.
author Sein-Echaluce, Violeta C.
author_facet Sein-Echaluce, Violeta C.
González, Andrés
Napolitano, Mauro
Luque, Ignacio
Barja, Francisco
Peleato, M.L.
Fillat, María F.
author_role author
author2 González, Andrés
Napolitano, Mauro
Luque, Ignacio
Barja, Francisco
Peleato, M.L.
Fillat, María F.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Bacteria
Ecophysiology
Environmental signal/stress responses
Gene expression/regulation
Metabolic networks
Metals
Microbial genetics
topic Bacteria
Ecophysiology
Environmental signal/stress responses
Gene expression/regulation
Metabolic networks
Metals
Microbial genetics
description Iron and zinc are necessary nutrients whose homeostasis is tightly controlled by members of the ferric uptake regulator (FUR) superfamily in the cyanobacterium Anabaena sp. PCC7120. Although the link between iron metabolism and oxidative stress management is well documented, little is known about the connection between zinc homeostasis and the oxidative stress response in cyanobacteria. Zinc homeostasis in Anabaena is controlled by Zur, also named FurB. When overexpressed in Escherichia coli, Zur (FurB) improved cell survival during oxidative stress. In order to investigate the possible correlation between Zur and the oxidative stress response in Anabaena, zur deletion and zur-overexpressing strains have been constructed, and the consequences of Zur imbalance evaluated. The lack of Zur increased sensitivity to hydrogen peroxide (H2 O2 ), whereas an excess of Zur enhanced oxidative stress resistance. Both mutants displayed pleiotropic phenotypes, including alterations on the filament surfaces observable by scanning electron microscopy, reduced content of endogenous H2 O2 and altered expression of sodA, catalases and several peroxiredoxins. Transcriptional and biochemical analyses unveiled that the appropriate level of Zur is required for proper control of the oxidative stress response and allowed us to identify major antioxidant enzymes as novel members of the Zur regulon.
publishDate 2015
dc.date.none.fl_str_mv 2015
2015
2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/117692
url http://hdl.handle.net/10261/117692
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1111/1462-2920.12628
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Wiley-Blackwell
publisher.none.fl_str_mv Wiley-Blackwell
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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