Characterization of the spectrum of trivalent VAV1-mutation-driven tumours using a gene-edited mouse model

[EN]Mutations in the VAV1 guanine nucleotide exchange factor 1 have been recently found in peripheral T cell lymphoma and nonsmall-cell lung cancer (NSCLC). To understand their pathogenic potential, we generated a gene-edited mouse model that expresses a VAV1 mutant protein that recapitulates the si...

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Detalles Bibliográficos
Autores: Robles Valero, Javier, Fernández-Nevado, Lucía, Cuadrado López, Myriam, Lorenzo Martín, L. Francisco, Fernández-Pisonero, Isabel, Abad, Antonio, Redín, Esther, Montuenga, Luis, Martín-Zanca, Dionisio, Bigas, Anna, Mallo, Moisés, Dosil Castro, Mercedes, Bustelo, Xosé R.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Universidad de Salamanca (USAL)
Repositorio:GREDOS. Repositorio Institucional de la Universidad de Salamanca
OAI Identifier:oai:gredos.usal.es:10366/168603
Acceso en línea:http://hdl.handle.net/10366/168603
Access Level:acceso abierto
Palabra clave:RAC1
TP53
Angioimmunoblastic T cell lymphoma
Follicular helper T cells
Nonsmall-cell lung cancer
Peripheral T cell lymphoma
Proto-Oncogene Proteins p21(ras)
Mutant Proteins
Mutation
Animals
Proto-Oncogene Proteins c-vav
Neoplasms
Mice
3207.13 Oncología
neoplasias
animales
proteínas protooncogénicas c-vav
ratones
mutación
proteínas mutantes
proteínas protooncogénicas p21(ras)
Descripción
Sumario:[EN]Mutations in the VAV1 guanine nucleotide exchange factor 1 have been recently found in peripheral T cell lymphoma and nonsmall-cell lung cancer (NSCLC). To understand their pathogenic potential, we generated a gene-edited mouse model that expresses a VAV1 mutant protein that recapitulates the signalling alterations present in the VAV1 mutant subclass most frequently found in tumours. We could not detect any overt tumourigenic process in those mice. However, the concurrent elimination of the Trp53 tumour suppressor gene in them drives T cell lymphomagenesis. This process represents an exacerbation of the normal functions that wild-type VAV1 plays in follicular helper T cells. We also found that, in combination with the Kras oncogene, the VAV1 mutant version favours progression of NSCLC. These data indicate that VAV1 mutations play critical, although highly cell-type-specific, roles in tumourigenesis. They also indicate that such functions are contingent on the mutational landscape of the tumours involved.