CL100 expression is down-regulated in advanced epithelial ovarian cancer and its re-expression decreases its malignant potential
Although early stage ovarian cancer can be effectively treated with surgery and chemotherapy, the majority of cases present with advanced disease, which remains essentially incurable. Unfortunately, little is known about the genes important for the development and progression of this disease. In thi...
| Autores: | , , , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2002 |
| País: | España |
| Institución: | Universidad de Navarra |
| Repositorio: | Dadun. Depósito Académico Digital de la Universidad de Navarra |
| Idioma: | inglés |
| OAI Identifier: | oai:dadun.unav.edu:10171/20183 |
| Acceso en línea: | https://hdl.handle.net/10171/20183 |
| Access Level: | acceso abierto |
| Palabra clave: | MKP-1 Ovarian Cancer Phosphatases Growth Motility |
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CL100 expression is down-regulated in advanced epithelial ovarian cancer and its re-expression decreases its malignant potentialManzano, R.G. (Ramón G.)|||/items/51fae546-90ba-4ba0-855c-dc1143662604Montuenga-Badia, L.M. (Luis M.)|||/items/4c999705-b2c9-45ac-ba13-3f18594ae596Dayton, M. (M.)|||/items/4b929380-3551-4105-aae4-e404c8de325aDent, P. (P.)|||/items/db82a58c-618e-4312-b077-98b08286d3bbKinoshita, I. (Ichiro)|||/items/7445386e-cfa4-4ab5-92ee-3ecf9c6bc118Vicent-Cambra, S. (Silvestre)|||/items/20c60fd9-edba-4dba-942c-3669eb079816Gardner, G.J. (Ginger J.)|||/items/69303766-04e6-444d-9c76-b579aee15062Nguyen, P. (PhuongMai)|||/items/b4e5777b-c32a-4e4c-aaa2-276d03df15d1Choi, Y.H. (Yung-Hyun)|||/items/22959b5b-3c34-4b8e-947f-6d2460fe794aTrepel, J. (Jane)|||/items/1ee593ac-2c68-4f4d-9480-6121249f9ce4Auersperg, N. (N.)|||/items/1d6fa4ff-34b6-4ba8-8cbc-e554efb8be8aBirrer, M.J. (Michael J.)|||/items/0fd36514-78ea-43e9-87bf-3ca6f72a44a9MKP-1OvarianCancerPhosphatasesGrowthMotilityAlthough early stage ovarian cancer can be effectively treated with surgery and chemotherapy, the majority of cases present with advanced disease, which remains essentially incurable. Unfortunately, little is known about the genes important for the development and progression of this disease. In this study, the expression of 68 phosphatases was determined in immortalized ovarian epithelial cells (IOSE) and compared to ovarian cancer cell lines. CL100, a dual specificity phosphatase, displayed 10-25-fold higher expression in normal compared to malignant ovarian cell lines. Immunohistochemical staining of normal ovaries and 68 ovarian cancer specimens confirmed this differential expression. Re-expression of CL100 in ovarian cancer cells decreased adherent and non-adherent cell growth and induced phenotypic changes including loss of filopodia and lamellipodia with an associated decrease in cell motility. Induced expression of CL100 in ovarian cancer cells suppressed intraperitoneal tumor growth in nude mice. These results show for the first time that CL100 expression is altered in human ovarian cancer, that CL100 expression changes cell morphology and motility, and that it suppresses intraperitoneal growth of human ovarian epithelial cancer. These data suggest that down-regulation of CL100 may play a role in the progression of human ovarian cancer.Nature Publishing GroupDadun. Depósito Académico Digital Universidad de Navarra20112011-12-1220022002-01-0120022002-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10171/20183reponame:Dadun. Depósito Académico Digital de la Universidad de Navarrainstname:Universidad de NavarraInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:dadun.unav.edu:10171/201832026-06-21T12:47:57Z |
| dc.title.none.fl_str_mv |
CL100 expression is down-regulated in advanced epithelial ovarian cancer and its re-expression decreases its malignant potential |
| title |
CL100 expression is down-regulated in advanced epithelial ovarian cancer and its re-expression decreases its malignant potential |
| spellingShingle |
CL100 expression is down-regulated in advanced epithelial ovarian cancer and its re-expression decreases its malignant potential Manzano, R.G. (Ramón G.)|||/items/51fae546-90ba-4ba0-855c-dc1143662604 MKP-1 Ovarian Cancer Phosphatases Growth Motility |
| title_short |
CL100 expression is down-regulated in advanced epithelial ovarian cancer and its re-expression decreases its malignant potential |
| title_full |
CL100 expression is down-regulated in advanced epithelial ovarian cancer and its re-expression decreases its malignant potential |
| title_fullStr |
CL100 expression is down-regulated in advanced epithelial ovarian cancer and its re-expression decreases its malignant potential |
| title_full_unstemmed |
CL100 expression is down-regulated in advanced epithelial ovarian cancer and its re-expression decreases its malignant potential |
| title_sort |
CL100 expression is down-regulated in advanced epithelial ovarian cancer and its re-expression decreases its malignant potential |
| dc.creator.none.fl_str_mv |
Manzano, R.G. (Ramón G.)|||/items/51fae546-90ba-4ba0-855c-dc1143662604 Montuenga-Badia, L.M. (Luis M.)|||/items/4c999705-b2c9-45ac-ba13-3f18594ae596 Dayton, M. (M.)|||/items/4b929380-3551-4105-aae4-e404c8de325a Dent, P. (P.)|||/items/db82a58c-618e-4312-b077-98b08286d3bb Kinoshita, I. (Ichiro)|||/items/7445386e-cfa4-4ab5-92ee-3ecf9c6bc118 Vicent-Cambra, S. (Silvestre)|||/items/20c60fd9-edba-4dba-942c-3669eb079816 Gardner, G.J. (Ginger J.)|||/items/69303766-04e6-444d-9c76-b579aee15062 Nguyen, P. (PhuongMai)|||/items/b4e5777b-c32a-4e4c-aaa2-276d03df15d1 Choi, Y.H. (Yung-Hyun)|||/items/22959b5b-3c34-4b8e-947f-6d2460fe794a Trepel, J. (Jane)|||/items/1ee593ac-2c68-4f4d-9480-6121249f9ce4 Auersperg, N. (N.)|||/items/1d6fa4ff-34b6-4ba8-8cbc-e554efb8be8a Birrer, M.J. (Michael J.)|||/items/0fd36514-78ea-43e9-87bf-3ca6f72a44a9 |
| author |
Manzano, R.G. (Ramón G.)|||/items/51fae546-90ba-4ba0-855c-dc1143662604 |
| author_facet |
Manzano, R.G. (Ramón G.)|||/items/51fae546-90ba-4ba0-855c-dc1143662604 Montuenga-Badia, L.M. (Luis M.)|||/items/4c999705-b2c9-45ac-ba13-3f18594ae596 Dayton, M. (M.)|||/items/4b929380-3551-4105-aae4-e404c8de325a Dent, P. (P.)|||/items/db82a58c-618e-4312-b077-98b08286d3bb Kinoshita, I. (Ichiro)|||/items/7445386e-cfa4-4ab5-92ee-3ecf9c6bc118 Vicent-Cambra, S. (Silvestre)|||/items/20c60fd9-edba-4dba-942c-3669eb079816 Gardner, G.J. (Ginger J.)|||/items/69303766-04e6-444d-9c76-b579aee15062 Nguyen, P. (PhuongMai)|||/items/b4e5777b-c32a-4e4c-aaa2-276d03df15d1 Choi, Y.H. (Yung-Hyun)|||/items/22959b5b-3c34-4b8e-947f-6d2460fe794a Trepel, J. (Jane)|||/items/1ee593ac-2c68-4f4d-9480-6121249f9ce4 Auersperg, N. (N.)|||/items/1d6fa4ff-34b6-4ba8-8cbc-e554efb8be8a Birrer, M.J. (Michael J.)|||/items/0fd36514-78ea-43e9-87bf-3ca6f72a44a9 |
| author_role |
author |
| author2 |
Montuenga-Badia, L.M. (Luis M.)|||/items/4c999705-b2c9-45ac-ba13-3f18594ae596 Dayton, M. (M.)|||/items/4b929380-3551-4105-aae4-e404c8de325a Dent, P. (P.)|||/items/db82a58c-618e-4312-b077-98b08286d3bb Kinoshita, I. (Ichiro)|||/items/7445386e-cfa4-4ab5-92ee-3ecf9c6bc118 Vicent-Cambra, S. (Silvestre)|||/items/20c60fd9-edba-4dba-942c-3669eb079816 Gardner, G.J. (Ginger J.)|||/items/69303766-04e6-444d-9c76-b579aee15062 Nguyen, P. (PhuongMai)|||/items/b4e5777b-c32a-4e4c-aaa2-276d03df15d1 Choi, Y.H. (Yung-Hyun)|||/items/22959b5b-3c34-4b8e-947f-6d2460fe794a Trepel, J. (Jane)|||/items/1ee593ac-2c68-4f4d-9480-6121249f9ce4 Auersperg, N. (N.)|||/items/1d6fa4ff-34b6-4ba8-8cbc-e554efb8be8a Birrer, M.J. (Michael J.)|||/items/0fd36514-78ea-43e9-87bf-3ca6f72a44a9 |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Dadun. Depósito Académico Digital Universidad de Navarra |
| dc.subject.none.fl_str_mv |
MKP-1 Ovarian Cancer Phosphatases Growth Motility |
| topic |
MKP-1 Ovarian Cancer Phosphatases Growth Motility |
| description |
Although early stage ovarian cancer can be effectively treated with surgery and chemotherapy, the majority of cases present with advanced disease, which remains essentially incurable. Unfortunately, little is known about the genes important for the development and progression of this disease. In this study, the expression of 68 phosphatases was determined in immortalized ovarian epithelial cells (IOSE) and compared to ovarian cancer cell lines. CL100, a dual specificity phosphatase, displayed 10-25-fold higher expression in normal compared to malignant ovarian cell lines. Immunohistochemical staining of normal ovaries and 68 ovarian cancer specimens confirmed this differential expression. Re-expression of CL100 in ovarian cancer cells decreased adherent and non-adherent cell growth and induced phenotypic changes including loss of filopodia and lamellipodia with an associated decrease in cell motility. Induced expression of CL100 in ovarian cancer cells suppressed intraperitoneal tumor growth in nude mice. These results show for the first time that CL100 expression is altered in human ovarian cancer, that CL100 expression changes cell morphology and motility, and that it suppresses intraperitoneal growth of human ovarian epithelial cancer. These data suggest that down-regulation of CL100 may play a role in the progression of human ovarian cancer. |
| publishDate |
2002 |
| dc.date.none.fl_str_mv |
2002 2002-01-01 2002 2002-01-01 2011 2011-12-12 |
| dc.type.none.fl_str_mv |
journal article http://purl.org/coar/resource_type/c_6501 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/10171/20183 |
| url |
https://hdl.handle.net/10171/20183 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 |
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info:eu-repo/semantics/openAccess |
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open access http://purl.org/coar/access_right/c_abf2 |
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openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Nature Publishing Group |
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Nature Publishing Group |
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reponame:Dadun. Depósito Académico Digital de la Universidad de Navarra instname:Universidad de Navarra |
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Universidad de Navarra |
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Dadun. Depósito Académico Digital de la Universidad de Navarra |
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Dadun. Depósito Académico Digital de la Universidad de Navarra |
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1869424901728763904 |
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15.301629 |