Halorientalis pallida sp. nov., an extremely halophilic archaeon isolated from a marine saltern

An extremely halophilic archaeon, strain F13-25T, was isolated from a marine saltern located in Isla Cristina, Huelva, on the south-west coast of Spain. The novel strain had pink-pigmented, non-motile, coccoid cells. Optimal growth was achieved at 25 % (w/v) NaCl, pH 7.5 and 37 °C. Strain F13-25T po...

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Autores: Durán Viseras, Ana, Sánchez-Porro Álvarez, Cristina, Ventosa Ucero, Antonio
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2019
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/164097
Acceso en línea:https://hdl.handle.net/11441/164097
https://doi.org/10.1099/ijsem.0.003675
Access Level:acceso abierto
Palabra clave:Haloarchaea
Halorientalis pallida
Hypersaline environments
Polar lipids
Saltern
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spelling Halorientalis pallida sp. nov., an extremely halophilic archaeon isolated from a marine salternDurán Viseras, AnaSánchez-Porro Álvarez, CristinaVentosa Ucero, AntonioHaloarchaeaHalorientalis pallidaHypersaline environmentsPolar lipidsSalternAn extremely halophilic archaeon, strain F13-25T, was isolated from a marine saltern located in Isla Cristina, Huelva, on the south-west coast of Spain. The novel strain had pink-pigmented, non-motile, coccoid cells. Optimal growth was achieved at 25 % (w/v) NaCl, pH 7.5 and 37 °C. Strain F13-25T possessed two heterogeneous 16S rRNA genes (rrnA and rrnB) most closely related to Halorientalis persicus D108T (97.6-99.2 % sequence similarity) and Halorientalis regularis TNN28T (95.9-98.8 %). On the basis of the results of rpoB' gene sequence analysis, strain F13-25T was also closely related to Halorientalis persicus IBRC-M 10043T (89.9 %) and Halorientalis regularis TNN28T (92.3 %). Relatedness values, computed using the Genome-to-Genome Distance Calculator, between strain F13-25T and Halorientalis persicus IBRC-M 10043T and Halorientalis regularis IBRC-M 10760T were 34.6 and 36.2 %, respectively. Average nucleotide identity values based on orthoANI, ANIb and ANIm of strain F13-25T and Halorientalis persicus IBRC-M 10043T and Halorientalisregularis IBRC-M 10760T were 88.0 and 88.8, 87.1 and 87.6 %, and 89.2 and 89.6 %, respectively. All values were far below the threshold accepted for prokaryotic species delineation. The major polar lipids were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and one glycolipid chromatographically identical to sulfated diglycosyl diether. The DNA G+C content was 65.7 mol% (genome). The results of phylogenetic, phenotypic and chemotaxonomic analyses indicated that strain F13-25T represents a novel species of the genus Halorientalis, for which the name Halorientalis pallida sp. nov., with type strain F13-25T (=CECT 9384T=IBRC-M 11176T), is proposed.Ministerio de Ciencia, Innovación y Universidades CGL2017-83385-PJunta de Andalucía BIO-213Microbiology SocietyMicrobiología y ParasitologíaMinisterio de Ciencia, Innovación y Universidades (MICINN). EspañaJunta de Andalucía2019info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/164097https://doi.org/10.1099/ijsem.0.003675reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésInternational Journal of Systematic and Evolutionary Microbiology, 69 (11), 3636-3643.CGL2017-83385-PBIO-213https://doi.org/10.1099/ijsem.0.003675info:eu-repo/semantics/openAccessoai:idus.us.es:11441/1640972026-06-17T12:51:07Z
dc.title.none.fl_str_mv Halorientalis pallida sp. nov., an extremely halophilic archaeon isolated from a marine saltern
title Halorientalis pallida sp. nov., an extremely halophilic archaeon isolated from a marine saltern
spellingShingle Halorientalis pallida sp. nov., an extremely halophilic archaeon isolated from a marine saltern
Durán Viseras, Ana
Haloarchaea
Halorientalis pallida
Hypersaline environments
Polar lipids
Saltern
title_short Halorientalis pallida sp. nov., an extremely halophilic archaeon isolated from a marine saltern
title_full Halorientalis pallida sp. nov., an extremely halophilic archaeon isolated from a marine saltern
title_fullStr Halorientalis pallida sp. nov., an extremely halophilic archaeon isolated from a marine saltern
title_full_unstemmed Halorientalis pallida sp. nov., an extremely halophilic archaeon isolated from a marine saltern
title_sort Halorientalis pallida sp. nov., an extremely halophilic archaeon isolated from a marine saltern
dc.creator.none.fl_str_mv Durán Viseras, Ana
Sánchez-Porro Álvarez, Cristina
Ventosa Ucero, Antonio
author Durán Viseras, Ana
author_facet Durán Viseras, Ana
Sánchez-Porro Álvarez, Cristina
Ventosa Ucero, Antonio
author_role author
author2 Sánchez-Porro Álvarez, Cristina
Ventosa Ucero, Antonio
author2_role author
author
dc.contributor.none.fl_str_mv Microbiología y Parasitología
Ministerio de Ciencia, Innovación y Universidades (MICINN). España
Junta de Andalucía
dc.subject.none.fl_str_mv Haloarchaea
Halorientalis pallida
Hypersaline environments
Polar lipids
Saltern
topic Haloarchaea
Halorientalis pallida
Hypersaline environments
Polar lipids
Saltern
description An extremely halophilic archaeon, strain F13-25T, was isolated from a marine saltern located in Isla Cristina, Huelva, on the south-west coast of Spain. The novel strain had pink-pigmented, non-motile, coccoid cells. Optimal growth was achieved at 25 % (w/v) NaCl, pH 7.5 and 37 °C. Strain F13-25T possessed two heterogeneous 16S rRNA genes (rrnA and rrnB) most closely related to Halorientalis persicus D108T (97.6-99.2 % sequence similarity) and Halorientalis regularis TNN28T (95.9-98.8 %). On the basis of the results of rpoB' gene sequence analysis, strain F13-25T was also closely related to Halorientalis persicus IBRC-M 10043T (89.9 %) and Halorientalis regularis TNN28T (92.3 %). Relatedness values, computed using the Genome-to-Genome Distance Calculator, between strain F13-25T and Halorientalis persicus IBRC-M 10043T and Halorientalis regularis IBRC-M 10760T were 34.6 and 36.2 %, respectively. Average nucleotide identity values based on orthoANI, ANIb and ANIm of strain F13-25T and Halorientalis persicus IBRC-M 10043T and Halorientalisregularis IBRC-M 10760T were 88.0 and 88.8, 87.1 and 87.6 %, and 89.2 and 89.6 %, respectively. All values were far below the threshold accepted for prokaryotic species delineation. The major polar lipids were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and one glycolipid chromatographically identical to sulfated diglycosyl diether. The DNA G+C content was 65.7 mol% (genome). The results of phylogenetic, phenotypic and chemotaxonomic analyses indicated that strain F13-25T represents a novel species of the genus Halorientalis, for which the name Halorientalis pallida sp. nov., with type strain F13-25T (=CECT 9384T=IBRC-M 11176T), is proposed.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/164097
https://doi.org/10.1099/ijsem.0.003675
url https://hdl.handle.net/11441/164097
https://doi.org/10.1099/ijsem.0.003675
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv International Journal of Systematic and Evolutionary Microbiology, 69 (11), 3636-3643.
CGL2017-83385-P
BIO-213
https://doi.org/10.1099/ijsem.0.003675
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Microbiology Society
publisher.none.fl_str_mv Microbiology Society
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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