Bread Wheat Doubled Haploid Production by Anther Culture

25 Pags.- 3 Figs.- 1 Tabl. The definitive version is available at: https://link.springer.com/book/10.1007/978-1-0716-1315-3

Detalhes bibliográficos
Autores: Castillo Alonso, Ana María, Valero-Rubira, Isabel, Allué, Sandra, Costar Castán, Asunción, Vallés Brau, María Pilar
Tipo de documento: outro
Estado:Versión aceptada para publicación
Data de publicação:2021
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositório:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/268685
Acesso em linha:http://hdl.handle.net/10261/268685
Access Level:Acceso aberto
Palavra-chave:Anther culture
Bread wheat
Colchicine
Doubled haploid
Microspore embryogenesis
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spelling Bread Wheat Doubled Haploid Production by Anther CultureCastillo Alonso, Ana MaríaValero-Rubira, IsabelAllué, SandraCostar Castán, AsunciónVallés Brau, María PilarAnther cultureBread wheatColchicineDoubled haploidMicrospore embryogenesis25 Pags.- 3 Figs.- 1 Tabl. The definitive version is available at: https://link.springer.com/book/10.1007/978-1-0716-1315-3The use of doubled haploid (DH) plants in plant breeding programmes is the fastest route to release new varieties (4–6 years), allowing for a rapid response to end-user needs. Microspore embryogenesis is one of the most efficient methods for DH plant production in bread wheat. In this process, microspores triggered by a stress treatment or by application of bioactive compounds are reprogrammed to follow an embryogenic pathway that leads to the production of haploid or DH plants. In this chapter, we describe a protocol for anther culture of bread wheat. This protocol is based on an osmotic and starvation treatment of the anthers followed by the application of a microtubule disrupting agent. Anthers are cultured in an ovary pre-conditioned medium with mature ovaries from cv. Caramba. This protocol has been applied to a wide range of genotypes and F1s from bread and spelt wheat.This research was supported by Projects AGL2013-46698-R and AGL2016-77211-R from State R&D Program Oriented to the Challenges of the Spanish Society, and funds for Reference Research Groups recognized by Diputación General de Aragón, Spain (Groups A06 and A08-20R: Genetics, Genomics, Biotechnology and Plant Breeding). Isabel Valero-Rubira was funded by the Spanish Ministry of Science and Innovation grant no. BES-2017-080970 (linked to Project AGL2016-77211-R).Peer reviewedSpringer NatureMinisterio de Economía y Competitividad (España)Gobierno de AragónMinisterio de Ciencia e Innovación (España)Castillo Alonso, Ana María [0000-0002-6143-3227]Vallés Brau, María Pilar [0000-0002-8649-2362]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202220222021info:eu-repo/semantics/otherhttp://purl.org/coar/resource_type/c_3248Postprintinfo:eu-repo/semantics/acceptedVersioninfo:eu-repo/semantics/bookParthttp://hdl.handle.net/10261/268685reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016MINECO/ICTI2013‐2016info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016MINECO/ICTI2013‐2016https://doi.org/10.1007/978-1-0716-1315-3_11Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2686852026-05-22T06:33:51Z
dc.title.none.fl_str_mv Bread Wheat Doubled Haploid Production by Anther Culture
title Bread Wheat Doubled Haploid Production by Anther Culture
spellingShingle Bread Wheat Doubled Haploid Production by Anther Culture
Castillo Alonso, Ana María
Anther culture
Bread wheat
Colchicine
Doubled haploid
Microspore embryogenesis
title_short Bread Wheat Doubled Haploid Production by Anther Culture
title_full Bread Wheat Doubled Haploid Production by Anther Culture
title_fullStr Bread Wheat Doubled Haploid Production by Anther Culture
title_full_unstemmed Bread Wheat Doubled Haploid Production by Anther Culture
title_sort Bread Wheat Doubled Haploid Production by Anther Culture
dc.creator.none.fl_str_mv Castillo Alonso, Ana María
Valero-Rubira, Isabel
Allué, Sandra
Costar Castán, Asunción
Vallés Brau, María Pilar
author Castillo Alonso, Ana María
author_facet Castillo Alonso, Ana María
Valero-Rubira, Isabel
Allué, Sandra
Costar Castán, Asunción
Vallés Brau, María Pilar
author_role author
author2 Valero-Rubira, Isabel
Allué, Sandra
Costar Castán, Asunción
Vallés Brau, María Pilar
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Gobierno de Aragón
Ministerio de Ciencia e Innovación (España)
Castillo Alonso, Ana María [0000-0002-6143-3227]
Vallés Brau, María Pilar [0000-0002-8649-2362]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Anther culture
Bread wheat
Colchicine
Doubled haploid
Microspore embryogenesis
topic Anther culture
Bread wheat
Colchicine
Doubled haploid
Microspore embryogenesis
description 25 Pags.- 3 Figs.- 1 Tabl. The definitive version is available at: https://link.springer.com/book/10.1007/978-1-0716-1315-3
publishDate 2021
dc.date.none.fl_str_mv 2021
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/other
http://purl.org/coar/resource_type/c_3248
Postprint
info:eu-repo/semantics/acceptedVersion
dc.type.openaire.fl_str_mv info:eu-repo/semantics/bookPart
format other
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/268685
url http://hdl.handle.net/10261/268685
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016MINECO/ICTI2013‐2016
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016MINECO/ICTI2013‐2016
https://doi.org/10.1007/978-1-0716-1315-3_11
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
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