A holistic approach to unravelling chondroitin sulfation: Correlations between surface charge, structure and binding to growth factors

Chondroitin sulfate (CS) is a relevant family of polysaccharides that participates in a large variety of biological events that are related to neural processes by regulating various growth factors through the pattern and degree of sulfation of the polysaccharide. However, their own complexity makes...

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Detalles Bibliográficos
Autores: Benito-Arenas, Raúl, Doncel-Pérez, Ernesto, Fernández-Gutiérrez, Mar, Garrido, Leoncio, García-Junceda, Eduardo, Revuelta, Julia, Bastida, Agatha, Fernández-Mayoralas, Alfonso
Tipo de recurso: artículo
Fecha de publicación:2018
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/172691
Acceso en línea:http://hdl.handle.net/10261/172691
Access Level:acceso abierto
Palabra clave:Structure-activity relationships
Chondroitin sulfate
Protein interactions
Glycosaminoglycan
Descripción
Sumario:Chondroitin sulfate (CS) is a relevant family of polysaccharides that participates in a large variety of biological events that are related to neural processes by regulating various growth factors through the pattern and degree of sulfation of the polysaccharide. However, their own complexity makes their optimization for biomedical applications a difficult undertaking. Thus, a different perspective has to be taken. Herein, we show that the particular sulfate distribution within the disaccharide repeating-unit plays a key role in the binding of growth factors (GFs). In particular, this disposition modulates the surface charge of the helical structure that, interestingly, has a significant influence on the binding capacity of CSs with several GFs. This fact should be carefully considered in the design of new ligands with improved activity as GFs ligands.