Olive-leaf extracts modulate inflammation and oxidative stress associated with human H. pylori infection

Helicobacter pylori (H. pylori) is one of the major human pathogens and the main cause of pathological damages that can progress from chronic gastritis to gastric cancer. During the coloni-zation of gastric mucosa, this bacterium provokes a strong inflammatory response and subsequent oxidative proce...

Descripción completa

Detalles Bibliográficos
Autores: Silván Jiménez, José Manuel, Guerrero-Hurtado, Esperanza, Gutiérrez-Docio, Alba, Alarcón Cavero, Teresa, Prodanov Prodanov, Marin, Martinez-Rodriguez, Adolfo J.
Tipo de recurso: artículo
Fecha de publicación:2021
País:España
Institución:Universidad Autónoma de Madrid
Repositorio:Biblos-e Archivo. Repositorio Institucional de la UAM
Idioma:inglés
OAI Identifier:oai:repositorio.uam.es:10486/704326
Acceso en línea:http://hdl.handle.net/10486/704326
https://dx.doi.org/10.3390/antiox10122030
Access Level:acceso abierto
Palabra clave:Antioxidant
Oleuropein
Elenolic Acid
Química
id ES_f77fb39d12528362f28eda205a1b5483
oai_identifier_str oai:repositorio.uam.es:10486/704326
network_acronym_str ES
network_name_str España
repository_id_str
spelling Olive-leaf extracts modulate inflammation and oxidative stress associated with human H. pylori infectionSilván Jiménez, José ManuelGuerrero-Hurtado, EsperanzaGutiérrez-Docio, AlbaAlarcón Cavero, TeresaProdanov Prodanov, MarinMartinez-Rodriguez, Adolfo J.AntioxidantOleuropeinElenolic AcidQuímicaHelicobacter pylori (H. pylori) is one of the major human pathogens and the main cause of pathological damages that can progress from chronic gastritis to gastric cancer. During the coloni-zation of gastric mucosa, this bacterium provokes a strong inflammatory response and subsequent oxidative process, which are associated with tissue damage. Therefore, the objective of this research was to evaluate the ability of two olive-leaf extracts (E1 and E2) to modulate the inflammatory response and oxidative stress in H. pylori-infected human gastric AGS cells. The obtained results showed that both extracts significantly decreased interleukin-8 (IL-8) secretion and reactive oxygen species (ROS) production in human gastric AGS cells. Both extracts also showed antibacterial activity against different H. pylori strains. HPLC-PAD-MS characterization demonstrated that extract E1 was mainly composed of highly hydrophilic compounds, such as hydroxytyrosol (HT) and its glu-cosides, and it was the most effective extract as an antibacterial agent. In contrast, extract E2 was composed mostly of moderately hydrophilic compounds, such as oleuropein (OLE), and it was more effective than extract E1 as an anti-inflammatory agent. Both extracts exhibited similar potential to decrease ROS production. These results show the importance of standardizing the extract composition according to the bioactive properties that should be potentiatedMDPIDepartamento de Medicina Preventiva y Salud Pública y MicrobiologíaDepartamento de Química Física AplicadaFacultad de MedicinaFacultad de Ciencias20212021-12-20research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10486/704326https://dx.doi.org/10.3390/antiox10122030reponame:Biblos-e Archivo. Repositorio Institucional de la UAMinstname:Universidad Autónoma de MadridInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:repositorio.uam.es:10486/7043262026-06-23T12:46:27Z
dc.title.none.fl_str_mv Olive-leaf extracts modulate inflammation and oxidative stress associated with human H. pylori infection
title Olive-leaf extracts modulate inflammation and oxidative stress associated with human H. pylori infection
spellingShingle Olive-leaf extracts modulate inflammation and oxidative stress associated with human H. pylori infection
Silván Jiménez, José Manuel
Antioxidant
Oleuropein
Elenolic Acid
Química
title_short Olive-leaf extracts modulate inflammation and oxidative stress associated with human H. pylori infection
title_full Olive-leaf extracts modulate inflammation and oxidative stress associated with human H. pylori infection
title_fullStr Olive-leaf extracts modulate inflammation and oxidative stress associated with human H. pylori infection
title_full_unstemmed Olive-leaf extracts modulate inflammation and oxidative stress associated with human H. pylori infection
title_sort Olive-leaf extracts modulate inflammation and oxidative stress associated with human H. pylori infection
dc.creator.none.fl_str_mv Silván Jiménez, José Manuel
Guerrero-Hurtado, Esperanza
Gutiérrez-Docio, Alba
Alarcón Cavero, Teresa
Prodanov Prodanov, Marin
Martinez-Rodriguez, Adolfo J.
author Silván Jiménez, José Manuel
author_facet Silván Jiménez, José Manuel
Guerrero-Hurtado, Esperanza
Gutiérrez-Docio, Alba
Alarcón Cavero, Teresa
Prodanov Prodanov, Marin
Martinez-Rodriguez, Adolfo J.
author_role author
author2 Guerrero-Hurtado, Esperanza
Gutiérrez-Docio, Alba
Alarcón Cavero, Teresa
Prodanov Prodanov, Marin
Martinez-Rodriguez, Adolfo J.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Departamento de Medicina Preventiva y Salud Pública y Microbiología
Departamento de Química Física Aplicada
Facultad de Medicina
Facultad de Ciencias
dc.subject.none.fl_str_mv Antioxidant
Oleuropein
Elenolic Acid
Química
topic Antioxidant
Oleuropein
Elenolic Acid
Química
description Helicobacter pylori (H. pylori) is one of the major human pathogens and the main cause of pathological damages that can progress from chronic gastritis to gastric cancer. During the coloni-zation of gastric mucosa, this bacterium provokes a strong inflammatory response and subsequent oxidative process, which are associated with tissue damage. Therefore, the objective of this research was to evaluate the ability of two olive-leaf extracts (E1 and E2) to modulate the inflammatory response and oxidative stress in H. pylori-infected human gastric AGS cells. The obtained results showed that both extracts significantly decreased interleukin-8 (IL-8) secretion and reactive oxygen species (ROS) production in human gastric AGS cells. Both extracts also showed antibacterial activity against different H. pylori strains. HPLC-PAD-MS characterization demonstrated that extract E1 was mainly composed of highly hydrophilic compounds, such as hydroxytyrosol (HT) and its glu-cosides, and it was the most effective extract as an antibacterial agent. In contrast, extract E2 was composed mostly of moderately hydrophilic compounds, such as oleuropein (OLE), and it was more effective than extract E1 as an anti-inflammatory agent. Both extracts exhibited similar potential to decrease ROS production. These results show the importance of standardizing the extract composition according to the bioactive properties that should be potentiated
publishDate 2021
dc.date.none.fl_str_mv 2021
2021-12-20
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10486/704326
https://dx.doi.org/10.3390/antiox10122030
url http://hdl.handle.net/10486/704326
https://dx.doi.org/10.3390/antiox10122030
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Biblos-e Archivo. Repositorio Institucional de la UAM
instname:Universidad Autónoma de Madrid
instname_str Universidad Autónoma de Madrid
reponame_str Biblos-e Archivo. Repositorio Institucional de la UAM
collection Biblos-e Archivo. Repositorio Institucional de la UAM
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869424888502026240
score 15,301603

Similares en LA Referencia