Effect of different killing methods during curing on the phytochemical and bacterial composition of Vanilla planifolia using multi-omic approaches

Vanilla planifolia Jacks. ex Andrews, is cultivated for its aromatic pods, obtaining the primary source of vanillin, a molecule valued for its flavor and bioactivity. Mexico ranks among the top five global producers, and Papantla, Veracruz, contributes 70 % of national production. Developing vanilla...

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Detalles Bibliográficos
Autores: Cuan-Escobar, Tiffany A., Cuellar-Sánchez, Alma, Gómez-Velázquez, Haiku D. J., Monribot-Villanueva, Juan L., Guerrero-Analco, José A., Gutiérrez-Díaz, Isabel, Luna-Vital, Diego A.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/384274
Acceso en línea:http://hdl.handle.net/10261/384274
https://api.elsevier.com/content/abstract/scopus_id/85217926283
Access Level:acceso abierto
Palabra clave:16S sequencing
Curing process
Killing process
Metabolomics
Phenolic compounds
Vanilla planifolia
Descripción
Sumario:Vanilla planifolia Jacks. ex Andrews, is cultivated for its aromatic pods, obtaining the primary source of vanillin, a molecule valued for its flavor and bioactivity. Mexico ranks among the top five global producers, and Papantla, Veracruz, contributes 70 % of national production. Developing vanilla's characteristic aroma involves a curing process composed of killing, sweating, drying, and conditioning, which enzymatic reactions and microbial activity play essential roles. This study assessed the impact of four killing treatments: microwave, hot water immersion, sonication, and freezing on the phenolic composition and bacterial communities in vanilla curing through metabolomic and 16S sequencing approaches. Freezing treatment resulted in the most substantial changes in phenolic profiles, including higher vanillin concentrations. Bacillus was the dominant bacterial genus, with hot water immersion and sonication showing the greatest α-diversity. These findings underscore the value of omic sciences in refining curing processes, enabling producers to achieve higher-quality vanilla through more efficient and technical methods.