The Effects of Interstitial Lung Diseases on Alveolar Extracellular Vesicles Profile

Diagnosis of interstitial lung diseases (ILD) is difficult to perform. Extracellular vesicles (EVs) facilitate cell-to-cell communication, and they are released by a variety of cells. Our goal aimed to investigate EV markers in bronchoalveolar lavage (BAL) from idiopathic pulmonary fibrosis (IPF), s...

Descripción completa

Detalles Bibliográficos
Autores: d'Alessandro, Miriana|||0000-0002-2368-5722, Gangi, Sara|||0000-0003-1834-535X, Soccio, Piera|||0000-0003-0151-9093, Cantó, Elisabet|||0000-0002-1782-1855, Osuna Gómez, Rubén|||0000-0003-2875-4405, Bergantini, Laura|||0000-0002-1118-3223, Cameli, Paolo|||0000-0001-8639-2882, Fabbri, Gaia, Croce, Sara, Scioscia, Giulia|||0000-0002-2667-077X, Montuori, Giusy, Fanetti, Matteo, Moriondo, Giorgia|||0000-0002-7517-916X, Mezzasalma, Fabrizio, Castillo, Diego|||0000-0002-4862-3595, Lacedonia, Donato|||0000-0003-0429-7967, Vidal, Silvia|||0000-0002-3909-6682, Bargagli, E.
Tipo de recurso: artículo
Fecha de publicación:2023
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:302823
Acceso en línea:https://ddd.uab.cat/record/302823
https://dx.doi.org/urn:doi:10.3390/ijms24044071
Access Level:acceso abierto
Palabra clave:Bronchoalveolar lavage
Diagnosis
Extracellular vesicles
Idiopathic pulmonary fibrosis
Interstitial lung diseases
Descripción
Sumario:Diagnosis of interstitial lung diseases (ILD) is difficult to perform. Extracellular vesicles (EVs) facilitate cell-to-cell communication, and they are released by a variety of cells. Our goal aimed to investigate EV markers in bronchoalveolar lavage (BAL) from idiopathic pulmonary fibrosis (IPF), sarcoidosis and hypersensitivity pneumonitis (HP) cohorts. ILD patients followed at Siena, Barcelona and Foggia University Hospitals were enrolled. BAL supernatants were used to isolate the EVs. They were characterized by flow cytometry assay through MACSPlex Exsome KIT. The majority of alveolar EV markers were related to the fibrotic damage. CD56, CD105, CD142, CD31 and CD49e were exclusively expressed by alveolar samples from IPF patients, while HP showed only CD86 and CD24. Some EV markers were common between HP and sarcoidosis (CD11c, CD1c, CD209, CD4, CD40, CD44, CD8). Principal component analysis distinguished the three groups based on EV markers with total variance of 60.08%. This study has demonstrated the validity of the flow cytometric method to phenotype and characterize EV surface markers in BAL samples. The two granulomatous diseases, sarcoidosis and HP, cohorts shared alveolar EV markers not revealed in IPF patients. Our findings demonstrated the viability of the alveolar compartment allowing identification of lung-specific markers for IPF and HP.

Similares en LA Referencia