An ordered pathway for the assembly of ESCRT-containing fungal ambient pH signalling complexes at the plasma membrane

12 páginas, 9 figuras -- PAGS nros. 1784-1795

Detalles Bibliográficos
Autores: Galindo, Antonio, Calcagno-Pizarelli, Ana María, Arst, Herbert Nathan Jr., Peñalva, Miguel Ángel
Tipo de recurso: artículo
Fecha de publicación:2012
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/49884
Acceso en línea:http://hdl.handle.net/10261/49884
Access Level:acceso abierto
Palabra clave:Arrestin
Seven transmembrane receptor
Multivesicular body pathway
Signal transduction
Aspergillus nidulans
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spelling An ordered pathway for the assembly of ESCRT-containing fungal ambient pH signalling complexes at the plasma membraneGalindo, AntonioCalcagno-Pizarelli, Ana MaríaArst, Herbert Nathan Jr.Peñalva, Miguel ÁngelArrestinSeven transmembrane receptorMultivesicular body pathwaySignal transductionAspergillus nidulans12 páginas, 9 figuras -- PAGS nros. 1784-1795The fungal pal/RIM signalling pathway, which regulates gene expression in response to environmental pH involves, in addition to dedicated proteins, several components of ESCRT complexes, which suggested that pH signalling proteins assemble on endosomal platforms. In Aspergillus nidulans, dedicated Pal proteins include the plasma membrane receptor PalH and its coupled arrestin, PalF, which becomes ubiquitylated in alkaline pH conditions, and three potentially endosomal ESCRT-III associates, including Vps32 interactors PalA and PalC and Vps24 interactor calpain-like PalB. We studied the subcellular locations at which signalling takes place after activating the pathway by shifting ambient pH to alkalinity. Rather than localising to endosomes, Vps32 interactors PalA and PalC transiently colocalise at alkaline-pH-induced cortical structures in a PalH-, Vps23- and Vps32-dependent but Vps27-independent manner. These cortical structures are much more stable when Vps4 is deficient, indicating that their half-life depends on ESCRT-III disassembly. Pull-down studies revealed that Vps23 interacts strongly with PalF, but co-immunoprecipitates exclusively with ubiquitylated PalF forms from extracts. We demonstrate that Vps23–GFP, expressed at physiological levels, is also recruited to cortical structures, very conspicuous in vps27Δ cells in which the prominent signal of Vps23–GFP on endosomes is eliminated, in a PalF- and alkaline pH-dependent manner. Dual-channel epifluorescence microscopy showed that PalC arrives at cortical complexes before PalA. As PalC recruitment is PalA independent and PalA recruitment is PalC dependent but PalB independent, these data complete the participation order of Pal proteins in the pathway and strongly support a model in which pH signalling takes place in ESCRT-containing, plasma-membrane-associated, rather than endosome-associated, complexesThis work was supported by Ministerio de Ciencia [grant number BIO2009-7281 to M.A.P.]; Comunidad de Madrid [grant number SAL/0246/2006 to M.A.P.]; the Biotechnology and Biological Sciences Research Council [grant numbers BB/D521781/1, BB/F01189X/1 to H.N.A. and, for the latter, Elaine Bignell]; and the Wellcome Trust [grant numbers 067878, 084660/Z/08/Z to H.N.A. and Joan TilburnPeer reviewedCompany of Biologists201220122012info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/49884reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/ 10.1242/​jcs.098897info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/498842026-05-22T06:33:51Z
dc.title.none.fl_str_mv An ordered pathway for the assembly of ESCRT-containing fungal ambient pH signalling complexes at the plasma membrane
title An ordered pathway for the assembly of ESCRT-containing fungal ambient pH signalling complexes at the plasma membrane
spellingShingle An ordered pathway for the assembly of ESCRT-containing fungal ambient pH signalling complexes at the plasma membrane
Galindo, Antonio
Arrestin
Seven transmembrane receptor
Multivesicular body pathway
Signal transduction
Aspergillus nidulans
title_short An ordered pathway for the assembly of ESCRT-containing fungal ambient pH signalling complexes at the plasma membrane
title_full An ordered pathway for the assembly of ESCRT-containing fungal ambient pH signalling complexes at the plasma membrane
title_fullStr An ordered pathway for the assembly of ESCRT-containing fungal ambient pH signalling complexes at the plasma membrane
title_full_unstemmed An ordered pathway for the assembly of ESCRT-containing fungal ambient pH signalling complexes at the plasma membrane
title_sort An ordered pathway for the assembly of ESCRT-containing fungal ambient pH signalling complexes at the plasma membrane
dc.creator.none.fl_str_mv Galindo, Antonio
Calcagno-Pizarelli, Ana María
Arst, Herbert Nathan Jr.
Peñalva, Miguel Ángel
author Galindo, Antonio
author_facet Galindo, Antonio
Calcagno-Pizarelli, Ana María
Arst, Herbert Nathan Jr.
Peñalva, Miguel Ángel
author_role author
author2 Calcagno-Pizarelli, Ana María
Arst, Herbert Nathan Jr.
Peñalva, Miguel Ángel
author2_role author
author
author
dc.subject.none.fl_str_mv Arrestin
Seven transmembrane receptor
Multivesicular body pathway
Signal transduction
Aspergillus nidulans
topic Arrestin
Seven transmembrane receptor
Multivesicular body pathway
Signal transduction
Aspergillus nidulans
description 12 páginas, 9 figuras -- PAGS nros. 1784-1795
publishDate 2012
dc.date.none.fl_str_mv 2012
2012
2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/49884
url http://hdl.handle.net/10261/49884
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/ 10.1242/​jcs.098897
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Company of Biologists
publisher.none.fl_str_mv Company of Biologists
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869424854896214016
score 15,811543

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