Role of factor H-related protein 3 in Pseudomonas aeruginosa bloodstream infections

Pseudomonas aeruginosa is a leading cause of nosocomial bloodstream infections. The outcome of these infections depends on the virulence of the microorganism as well as host-related conditions and factors. The complement system plays a crucial role in defense against bloodstream infections. P. aerug...

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Detalles Bibliográficos
Autores: Gonzalez-Alsina, Alex, Martín-Merinero, Héctor, Mateu-Borrás, Margalida, Verd, María, Doménech-Sánchez, Antonio, Goldberg, Joanna B, Rodriguez de Cordoba, Santiago, Alberti, Sebastian
Tipo de recurso: artículo
Fecha de publicación:2024
País:España
Institución:Conselleria de Salut i Consum del Govern de les Illes Balears
Repositorio:Docusalut
Idioma:inglés
OAI Identifier:oai:docusalut.com:20.500.13003/21126
Acceso en línea:https://hdl.handle.net/20.500.13003/21126
Access Level:acceso abierto
Palabra clave:Bacteremia
Bacterial Proteins
Complement Factor H
Humans
Host-Pathogen Interactions
Protein Binding
Pseudomonas aeruginosa
Pseudomonas Infections
Complement Activation
Activación de Complemento
Interacciones Huésped-Patógeno
Humanos
Infecciones por Pseudomonas
Bacteriemia
Proteínas Bacterianas
Unión Proteica
Factor H de Complemento
Descripción
Sumario:Pseudomonas aeruginosa is a leading cause of nosocomial bloodstream infections. The outcome of these infections depends on the virulence of the microorganism as well as host-related conditions and factors. The complement system plays a crucial role in defense against bloodstream infections. P. aeruginosa counteracts complement attack by recruiting Factor H (FH) that inhibits complement amplification on the bacterial surface. Factor H-related proteins (FHRs) are a group of plasma proteins evolutionarily related to FH that have been postulated to interfere this bacterial evasion mechanism. In this study, we demonstrate that FHR-3 competes with purified FH for binding to P. aeruginosa and identify EF-Tu as a common bacterial target for both complement regulator factors. Importantly, elevated levels of FHR-3 in human serum promote complement activation, leading to increased opsonization and killing of P. aeruginosa. Conversely, physiological concentrations of FHR-3 have no significant effect. Our findings suggest that FHR-3 may serve as a protective host factor against P. aeruginosa infections.