HVCN1 channels are relevant for the maintenance of sperm motility during in vitro capacitation of pig spermatozoa

The objective of the present study was to determine the physiological role of voltage-gated hydrogen channels 1 (HVCN1 channels) during in vitro capacitation of pig spermatozoa. Sperm samples from 20 boars were incubated in capacitating medium for 300 minutes (min) in the presence of 2-guanidino ben...

Descripción completa

Detalles Bibliográficos
Autores: Yeste Oliveras, Marc, Llavanera, Marc, Mateo Otero, Yentel, Catalán, Jaime, Bonet, Sergi, Pinart Nadal, Elisabeth
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10256/18074
Acceso en línea:http://hdl.handle.net/10256/18074
Access Level:acceso abierto
Palabra clave:Senglar -- Espermatozoides
Wild boar -- Spermatozoa
Senglar -- Fecunditat
Wild boar -- Fertility
Senglar -- Reproducció
Wild boar -- Reproduction
id ES_f6e76aebb97ac8a8721fad47a5dabb61
oai_identifier_str oai:recercat.cat:10256/18074
network_acronym_str ES
network_name_str España
repository_id_str
spelling HVCN1 channels are relevant for the maintenance of sperm motility during in vitro capacitation of pig spermatozoaYeste Oliveras, MarcLlavanera, MarcMateo Otero, YentelCatalán, JaimeBonet, SergiPinart Nadal, ElisabethSenglar -- EspermatozoidesWild boar -- SpermatozoaSenglar -- FecunditatWild boar -- FertilitySenglar -- ReproduccióWild boar -- ReproductionThe objective of the present study was to determine the physiological role of voltage-gated hydrogen channels 1 (HVCN1 channels) during in vitro capacitation of pig spermatozoa. Sperm samples from 20 boars were incubated in capacitating medium for 300 minutes (min) in the presence of 2-guanidino benzimidazole (2-GBI), a specific HVCN1-channel blocker, added either at 0 min or after 240 min of incubation. Control samples were incubated in capacitating medium without the inhibitor. In all samples, acrosomal exocytosis was triggered with progesterone after 240 min of incubation. Sperm viability, sperm motility and kinematics, acrosomal exocytosis, membrane lipid disorder, intracellular calcium levels and mitochondrial membrane potential were evaluated after 0, 60, 120, 180, 240, 250, 270 and 300 min of incubation. While HVCN1-blockage resulted in altered sperm viability, sperm motility and kinematics and reduced mitochondrial membrane potential as compared to control samples, at any blocker concentration and incubation time, it had a non-significant effect on intracellular Ca2+ levels determined through Fluo3-staining. The effects on acrosomal exocytosis were only significant in blocked samples at 0 min, and were associated with increased membrane lipid disorder and Ca2+ levels of the sperm head determined through Rhod5-staining. In conclusion, HVCN1 channels play a crucial role in the modulation of sperm motility and kinematics, and in Ca2+ entrance to the sperm headMDPI (Multidisciplinary Digital Publishing Institute)2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionpeer-reviewedapplication/pdfhttp://hdl.handle.net/10256/18074http://hdl.handle.net/10256/18074International Journal of Molecular Sciences, 2020, vol. 21, núm. 9, p. 3255Articles publicats (D-B)Yeste Oliveras, Marc Llavanera, Marc Mateo Otero, Yentel Catalán, Jaime Bonet, Sergi Pinart Nadal, Elisabeth 2020 HVCN1 channels are relevant for the maintenance of sperm motility during in vitro capacitation of pig spermatozoa International Journal of Molecular Sciences 21 9 3255reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)Inglésinfo:eu-repo/semantics/altIdentifier/doi/10.3390/ijms21093255info:eu-repo/semantics/altIdentifier/eissn/1422-0067Reconeixement 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessoai:recercat.cat:10256/180742026-05-29T05:05:01Z
dc.title.none.fl_str_mv HVCN1 channels are relevant for the maintenance of sperm motility during in vitro capacitation of pig spermatozoa
title HVCN1 channels are relevant for the maintenance of sperm motility during in vitro capacitation of pig spermatozoa
spellingShingle HVCN1 channels are relevant for the maintenance of sperm motility during in vitro capacitation of pig spermatozoa
Yeste Oliveras, Marc
Senglar -- Espermatozoides
Wild boar -- Spermatozoa
Senglar -- Fecunditat
Wild boar -- Fertility
Senglar -- Reproducció
Wild boar -- Reproduction
title_short HVCN1 channels are relevant for the maintenance of sperm motility during in vitro capacitation of pig spermatozoa
title_full HVCN1 channels are relevant for the maintenance of sperm motility during in vitro capacitation of pig spermatozoa
title_fullStr HVCN1 channels are relevant for the maintenance of sperm motility during in vitro capacitation of pig spermatozoa
title_full_unstemmed HVCN1 channels are relevant for the maintenance of sperm motility during in vitro capacitation of pig spermatozoa
title_sort HVCN1 channels are relevant for the maintenance of sperm motility during in vitro capacitation of pig spermatozoa
dc.creator.none.fl_str_mv Yeste Oliveras, Marc
Llavanera, Marc
Mateo Otero, Yentel
Catalán, Jaime
Bonet, Sergi
Pinart Nadal, Elisabeth
author Yeste Oliveras, Marc
author_facet Yeste Oliveras, Marc
Llavanera, Marc
Mateo Otero, Yentel
Catalán, Jaime
Bonet, Sergi
Pinart Nadal, Elisabeth
author_role author
author2 Llavanera, Marc
Mateo Otero, Yentel
Catalán, Jaime
Bonet, Sergi
Pinart Nadal, Elisabeth
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Senglar -- Espermatozoides
Wild boar -- Spermatozoa
Senglar -- Fecunditat
Wild boar -- Fertility
Senglar -- Reproducció
Wild boar -- Reproduction
topic Senglar -- Espermatozoides
Wild boar -- Spermatozoa
Senglar -- Fecunditat
Wild boar -- Fertility
Senglar -- Reproducció
Wild boar -- Reproduction
description The objective of the present study was to determine the physiological role of voltage-gated hydrogen channels 1 (HVCN1 channels) during in vitro capacitation of pig spermatozoa. Sperm samples from 20 boars were incubated in capacitating medium for 300 minutes (min) in the presence of 2-guanidino benzimidazole (2-GBI), a specific HVCN1-channel blocker, added either at 0 min or after 240 min of incubation. Control samples were incubated in capacitating medium without the inhibitor. In all samples, acrosomal exocytosis was triggered with progesterone after 240 min of incubation. Sperm viability, sperm motility and kinematics, acrosomal exocytosis, membrane lipid disorder, intracellular calcium levels and mitochondrial membrane potential were evaluated after 0, 60, 120, 180, 240, 250, 270 and 300 min of incubation. While HVCN1-blockage resulted in altered sperm viability, sperm motility and kinematics and reduced mitochondrial membrane potential as compared to control samples, at any blocker concentration and incubation time, it had a non-significant effect on intracellular Ca2+ levels determined through Fluo3-staining. The effects on acrosomal exocytosis were only significant in blocked samples at 0 min, and were associated with increased membrane lipid disorder and Ca2+ levels of the sperm head determined through Rhod5-staining. In conclusion, HVCN1 channels play a crucial role in the modulation of sperm motility and kinematics, and in Ca2+ entrance to the sperm head
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
peer-reviewed
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10256/18074
http://hdl.handle.net/10256/18074
url http://hdl.handle.net/10256/18074
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3390/ijms21093255
info:eu-repo/semantics/altIdentifier/eissn/1422-0067
dc.rights.none.fl_str_mv Reconeixement 4.0 Internacional
http://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Reconeixement 4.0 Internacional
http://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI (Multidisciplinary Digital Publishing Institute)
publisher.none.fl_str_mv MDPI (Multidisciplinary Digital Publishing Institute)
dc.source.none.fl_str_mv International Journal of Molecular Sciences, 2020, vol. 21, núm. 9, p. 3255
Articles publicats (D-B)
Yeste Oliveras, Marc Llavanera, Marc Mateo Otero, Yentel Catalán, Jaime Bonet, Sergi Pinart Nadal, Elisabeth 2020 HVCN1 channels are relevant for the maintenance of sperm motility during in vitro capacitation of pig spermatozoa International Journal of Molecular Sciences 21 9 3255
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869424806541131776
score 15.812429

Similares en LA Referencia