Alteration of light-dependent gene regulation by the absence of the RCO-1/RCM-1 repressor complex in the fungus Neurospora crassa

The activation of transcription by light in the fungus Neurospora crassa requires the White Collar Complex (WCC), a photoreceptor and transcription factor complex. After light reception two WCCs interact and bind the promoters of light- regulated genes to activate transcription. This process is regu...

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Autores: Ruger Herreros, Carmen, Gil Sánchez, María del Mar, Sancar, Gencer, Brunner, Michael, Corrochano Peláez, Luis María
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2014
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/40566
Acceso en línea:http://hdl.handle.net/11441/40566
https://doi.org/10.1371/journal.pone.0095069
Access Level:acceso abierto
Palabra clave:Cyclic AMP dependent protein kinase
Messenger RNA
RCM 1 protein
RCO 1 protein
Repressor protein
Unclassified drug
Fungal protein
Primer DNA
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spelling Alteration of light-dependent gene regulation by the absence of the RCO-1/RCM-1 repressor complex in the fungus Neurospora crassaRuger Herreros, CarmenGil Sánchez, María del MarSancar, GencerBrunner, MichaelCorrochano Peláez, Luis MaríaCyclic AMP dependent protein kinaseMessenger RNARCM 1 proteinRCO 1 proteinRepressor proteinUnclassified drugFungal proteinPrimer DNAThe activation of transcription by light in the fungus Neurospora crassa requires the White Collar Complex (WCC), a photoreceptor and transcription factor complex. After light reception two WCCs interact and bind the promoters of light- regulated genes to activate transcription. This process is regulated by VVD, a small photoreceptor that disrupts the interaction between WCCs and leads to a reduction in transcription after long exposures to light. The N. crassa RCO-1/RCM-1 repressor complex is the homolog of the Tup1-Ssn6 repressor complex in yeast, and its absence modifies photoadaptation. We show that the absence of the RCO-1/RCM-1 repressor complex leads to several alterations in transcription that are gene-specific: an increase in the accumulation of mRNAs in the dark, a repression of transcription, and a derepression of transcription after long exposures to light. The absence of the RCO-1/RCM-1 repressor complex leads to lower VVD levels that are available for the regulation of the activity of the WCC. The reduction in the amount of VVD results in increased WCC binding to the promoters of light-regulated genes in the dark and after long exposures to light, leading to the modification of photoadaptation that has been observed in rco-1 and rcm-1 mutants. Our results show that the photoadaptation phenotype of mutants in the RCO-1/RCM-1 repressor complex is, at least in part, an indirect consequence of the reduction of vvd transcription, and the resulting modification in the regulation of transcription by the WCCPublic Library of ScienceGenética2014info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/11441/40566https://doi.org/10.1371/journal.pone.0095069reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésPLoS One, 9,  e9506910.1371/journal.pone.0095069info:eu-repo/semantics/openAccessoai:idus.us.es:11441/405662026-06-17T12:51:07Z
dc.title.none.fl_str_mv Alteration of light-dependent gene regulation by the absence of the RCO-1/RCM-1 repressor complex in the fungus Neurospora crassa
title Alteration of light-dependent gene regulation by the absence of the RCO-1/RCM-1 repressor complex in the fungus Neurospora crassa
spellingShingle Alteration of light-dependent gene regulation by the absence of the RCO-1/RCM-1 repressor complex in the fungus Neurospora crassa
Ruger Herreros, Carmen
Cyclic AMP dependent protein kinase
Messenger RNA
RCM 1 protein
RCO 1 protein
Repressor protein
Unclassified drug
Fungal protein
Primer DNA
title_short Alteration of light-dependent gene regulation by the absence of the RCO-1/RCM-1 repressor complex in the fungus Neurospora crassa
title_full Alteration of light-dependent gene regulation by the absence of the RCO-1/RCM-1 repressor complex in the fungus Neurospora crassa
title_fullStr Alteration of light-dependent gene regulation by the absence of the RCO-1/RCM-1 repressor complex in the fungus Neurospora crassa
title_full_unstemmed Alteration of light-dependent gene regulation by the absence of the RCO-1/RCM-1 repressor complex in the fungus Neurospora crassa
title_sort Alteration of light-dependent gene regulation by the absence of the RCO-1/RCM-1 repressor complex in the fungus Neurospora crassa
dc.creator.none.fl_str_mv Ruger Herreros, Carmen
Gil Sánchez, María del Mar
Sancar, Gencer
Brunner, Michael
Corrochano Peláez, Luis María
author Ruger Herreros, Carmen
author_facet Ruger Herreros, Carmen
Gil Sánchez, María del Mar
Sancar, Gencer
Brunner, Michael
Corrochano Peláez, Luis María
author_role author
author2 Gil Sánchez, María del Mar
Sancar, Gencer
Brunner, Michael
Corrochano Peláez, Luis María
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Genética
dc.subject.none.fl_str_mv Cyclic AMP dependent protein kinase
Messenger RNA
RCM 1 protein
RCO 1 protein
Repressor protein
Unclassified drug
Fungal protein
Primer DNA
topic Cyclic AMP dependent protein kinase
Messenger RNA
RCM 1 protein
RCO 1 protein
Repressor protein
Unclassified drug
Fungal protein
Primer DNA
description The activation of transcription by light in the fungus Neurospora crassa requires the White Collar Complex (WCC), a photoreceptor and transcription factor complex. After light reception two WCCs interact and bind the promoters of light- regulated genes to activate transcription. This process is regulated by VVD, a small photoreceptor that disrupts the interaction between WCCs and leads to a reduction in transcription after long exposures to light. The N. crassa RCO-1/RCM-1 repressor complex is the homolog of the Tup1-Ssn6 repressor complex in yeast, and its absence modifies photoadaptation. We show that the absence of the RCO-1/RCM-1 repressor complex leads to several alterations in transcription that are gene-specific: an increase in the accumulation of mRNAs in the dark, a repression of transcription, and a derepression of transcription after long exposures to light. The absence of the RCO-1/RCM-1 repressor complex leads to lower VVD levels that are available for the regulation of the activity of the WCC. The reduction in the amount of VVD results in increased WCC binding to the promoters of light-regulated genes in the dark and after long exposures to light, leading to the modification of photoadaptation that has been observed in rco-1 and rcm-1 mutants. Our results show that the photoadaptation phenotype of mutants in the RCO-1/RCM-1 repressor complex is, at least in part, an indirect consequence of the reduction of vvd transcription, and the resulting modification in the regulation of transcription by the WCC
publishDate 2014
dc.date.none.fl_str_mv 2014
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11441/40566
https://doi.org/10.1371/journal.pone.0095069
url http://hdl.handle.net/11441/40566
https://doi.org/10.1371/journal.pone.0095069
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv PLoS One, 9,  e95069
10.1371/journal.pone.0095069
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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